Skip to main content
Springer Nature Link
Log in

Morphology and ultrastructure of trichomes of intact and in vitro plants of stevia rebaudiana Bertoni with reference to biosynthesis and accumulation of steviol glycosides

  • Published:
Moscow University Biological Sciences Bulletin Aims and scope Submit manuscript

Abstract

The ultrastructure of trichomes of intact and in vitro plants of stevia were investigated under scanning and transmission electronic microscopy. Three types of trichomes were found on both the upper and lower surfaces of the leaf: viz. rounded glands and large and small trichome. The density of trichomes on the surface of upper leaves is higher than on lower leaves, while in vitro plants are characterized with lower density of trichomes than intact ones. The positive correlation between number of trichomes on leaves and content of steviol glycosides (SGs) in them was found. Three basic stages of ontogeny of gland cells were revealed which reflect the sequence of secrete accumulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. Kinghorn, A.D. and Soejarto, D.D., Sweetening Agents of Plant Origin, Critical Rev. Plant Sci., 1986, vol. 4, no. 2, pp. 79–120.

    Article  CAS  Google Scholar 

  2. Lyakhovkin, A.G., Long, T.D., Titov, D.A., and Anh, M.P., Cultivation and Utilization of Stevia (Stevia rebaudiana Bertoni). Hanoi: Agricult. Publ. House, 1993, p. 44.

    Google Scholar 

  3. Matsui, M., Matsui, K., Kawasaki, Y., Oda, Y., Noguchi, T., Kitagawa, Y., Sawada, M., Hayashi, M., Nohmi, T., Yoshihira, K., Ishidate, M., and Sofuni, T., Evaluation of the Genotoxicity of Stevioside and Steviol using Six in vitro and One in vivo Mutagenicity Assays, Mutagenesis, 1996, vol. 11, no. 6, pp. 573–579.

    Article  CAS  PubMed  Google Scholar 

  4. Zaidan, L.B.P., Dietrich, S.M.C., and Felippe, G.M., Effect of Photoperiod on Flowering and Stevioside Content in Plants of Stevia rebaudiana Bertoni, Jap. J. Crop Sci., 1980, vol. 49, pp. 569–574.

    Google Scholar 

  5. Hsing, Y., Su, W., and Chang, W., Accumulation of Stevioside and Rebaudioside A in Callus Cultures of Stevia rebadiana Bertoni, Bot. Bull. Acad. Sin., 1983, vol. 24, pp. 115–119.

    CAS  Google Scholar 

  6. Darise, M., Kohda, H., Mizutani, K., Kasai, R., and Tanaka, P., Chemical Constituents of Flowers of Stevia rebaudiana Bertoni, Agric. Biol. Chem., 1983, vol. 47, pp. 133–135.

    CAS  Google Scholar 

  7. Bondarev, N., Reshetnyak, P., and Nosov, A., Peculiarities of Diterpenoid Steviol Glycoside Production in vitro Cultures of Stevia rebaudiana Bertoni, Plant Sci., 2001, vol. 161, no. 1, pp. 155–163.

    Article  CAS  Google Scholar 

  8. Bondarev, N., Sukhanova, M.A., Reshetnayk, P., and Nosov, A., Steviol Glycoside Content in Different Organs of Stevia rebaudiana Bertoni and Its Dynamics during Ontogenesis, Biol. Plant., 2004, vol. 47, no. 2, pp. 261–264.

    Article  Google Scholar 

  9. Hohmann, B., Botanisch-Warenkudliche Diognostik von Stevia rebaudiana (Bertoni) Hemsl., Einer Sustoffiefernden Pflanze, Deutsche Lebensmittel-Rundschau, 1978, vol. 74, no. 8, pp. 296–299.

    Google Scholar 

  10. Shaffert, E., Chebotar’, A., and Novikova, V., Morphoanatomical Characteristic of Stevia (Stevia rebaudiana (Vertoni) Hemsl.) in Relation to Its Introduction on the Southern Coast of Crimea, in Tsitologo-Embriologicheskie Issledovaniya Vysshikh Rastenii: Tr. Nikitskogo Bot. Sada (Cytological-Embryological Studies of Higher Plants, Transactions of the Nikita Botanical Garden), Yalta, 1992, vol. 113, pp. 25–37.

    Google Scholar 

  11. Danilova, M.F. and Kashina, T.K., Strukturnye osnovy aktinoritmicheskoi regulyatsii tsveteniya (Structural Basics of Actinorhythmic Regulation of Flowering), St. Petersburg: Nauka, 1999.

    Google Scholar 

  12. Dzyuba, O.O., Steviya (Stevia rebaudiana (Vertoni) Hemsleu): Introduction, Morphology, Biology, and Cultivation, Extended Abstract of Cand. Sci. (Biol.) Dissertation, St. Petersburg: VNII im. N.I. Vavilova, 1999.

    Google Scholar 

  13. Reynolds, E.C., The Use of Lead Citrate at High pH as an Electron-Opaque Stain in Electron Microscopy, J. Cell Biol., 1963, vol. 17, pp. 208–212.

    Article  CAS  PubMed  Google Scholar 

  14. Bondarev, N.I., Reshetnayk, O.V., and Nosov, A.M., Features of Development of Stevia rebaudiana Shoots Cultivated in the Roller Bioreactor and Their Production of Steviol Glycoside, Planta Med., 2002, vol. 68, pp. 759–762.

    Article  CAS  PubMed  Google Scholar 

  15. Vakhrusheva, T.E., Gogichaishvili, N.B., Gorbatenko, L.E., Dzyuba, O.O., and Talalova, E.E., Biochemical Characteristics of Stevia Depending on Cultivation Conditions, in Mater. II Mezhdunar. simpoz. “Novye i netraditsionnye rasteniya i perspektivy ikh prakticheskogo ispol’zovaniya”. T. 2 (Proc. II Int. Symp. “New and Unconventional Plants and Prospects of Their Practical Use), Pushchino, 1997, pp. 617–618.

  16. Sukhanova, M.A., Bondarev, N.I., Goryaeva, O.V., Andreeva, S.E., and Eosov, A.M., Ultrastructural Characteristics of Cells of Plants and Callus Cultures of Stevia rebaudiana in Relation to the Synthesis of Steviol Glycosides, Biotekhnologiya, 2007, vol. 5, pp. 51–59.

    Google Scholar 

  17. Ladygin, V.G., Bondarev, N.I., Semenova, G.A., Smolov, A.A., Resetnayk, O.V., and Nosov, A.M., Chloroplast Ultrastructure, Photosynthetic Apparatus Activities and Production of Steviol Glycosides in Stevia rebaudiana in vivo and in vitro, Biol. Plant., 2008, vol. 52, no. 1, pp. 9–16.

    Article  CAS  Google Scholar 

  18. Ascensao, L., Figueiredo, A.C., Barroso, J.G., Pedro, L.G., Schripsema, J., Deans, S.G., and Scheffer, J.C., Plectranthus madagascariensis: Morphology of the Glandular Trichomes, Essential Oil Composition, and Its Biological Activity, Int. J. Plant Sci., 1998, vol. 159, no. 1, pp. 31–38.

    Article  CAS  Google Scholar 

  19. Wagner, G.J., Secreting Glandular Trichomes: More Than Just Hairs, Plant Physiol., 1991, vol. 96, pp. 675–679.

    Article  CAS  PubMed  Google Scholar 

  20. Duke, S.O. and Paul, R.N., Development and Fine Structure of the Glandular Trichomes of Artemisia annua L., Int. J. Plant Sci., 1993, vol. 154, pp. 107–118.

    Article  Google Scholar 

  21. Vasil’ev, A.E., Funktsional’naya morflogiya sekretornykh kletok rastenii (Functional Morphology of Secretory Plant Cells), Leningrad: Nauka, 1977.

    Google Scholar 

  22. Denisova, G.A., Terpenosoderzhashchie struktury rastenii, Leningrad: Nauka, 1989.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    N. I. Bondarev & M. A. Sukhanova

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290, Russia

    G. A. Semenova

  3. Department of Plant Physiology, Moscow State University, Moscow, 119991, Russia

    O. V. Goryaeva, S. E. Andreeva & A. M. Nosov

Authors
  1. N. I. Bondarev
    View author publications

    Search author on:PubMed Google Scholar

  2. M. A. Sukhanova
    View author publications

    Search author on:PubMed Google Scholar

  3. G. A. Semenova
    View author publications

    Search author on:PubMed Google Scholar

  4. O. V. Goryaeva
    View author publications

    Search author on:PubMed Google Scholar

  5. S. E. Andreeva
    View author publications

    Search author on:PubMed Google Scholar

  6. A. M. Nosov
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to N. I. Bondarev.

Additional information

Original Russian Text © N.I. Bondarev, M.A. Sukhanova, G.A. Semenova, O.V. Goryaeva, S.E. Andreeva, A.M. Nosov, 2010, published in Vestnik Moskovskogo Universiteta. Biologiya, 2010, No. 1, pp. 15–20.

About this article

Cite this article

Bondarev, N.I., Sukhanova, M.A., Semenova, G.A. et al. Morphology and ultrastructure of trichomes of intact and in vitro plants of stevia rebaudiana Bertoni with reference to biosynthesis and accumulation of steviol glycosides. Moscow Univ. Biol.Sci. Bull. 65, 12–16 (2010). https://doi.org/10.3103/S0096392510010037

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0096392510010037

Keywords