Abstract
The ability to synthesize polyisoprene was studied for lines of Ficus elastica photoheterotrophic callus culture which results from the selection and prolonged cultivation of the culture in light in a medium containing 1% sucrose, 0.5 and 0.05 mg/l BAP or in the absence of the hormone. The results of microscopy study show that F. elastica lines are able to synthesize polyisoprene typical of intact plants. The substantial accumulation of polyisoprene was observed for the culture grown in the BAP-free media. Moreover, for some lines considered, tracheid structures were shown to be present in tissues. The formation of tracheid structures correlated directly with polyisoprene synthesis. We conclude that varying BAP content in the cultivation medium and selection allow the preparation of a culture exhibiting a certain functional specificity. Under optimal cultivation conditions, the culture develops toward the intensification of polyisoprene synthesis.
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Original Russian Text © I.B. Kol’chugina, E.N. Markarova, 2009, published in Vestnik Moskovskogo Universiteta. Biologiya, 2009, No. 1, pp. 31–35.
To prepare Javel water 20 g of sodium hypochloride was dissolved in 100 ml of distilled water and decanted; 15 g of potassium or sodium carbonate was dissolved in 10 ml of distilled water. The two solutions were mixed together and left for four days in a dark place. The mixture was filtrated through a double layer of linen using Buchner funnel and then, through a filter glass number three.
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Kol’chugina, I.B., Markarova, E.N. Photoheterotrophic callus culture Ficus elastica. The formation of polyisoprene synthesis. Moscow Univ. Biol.Sci. Bull. 64, 28–31 (2009). https://doi.org/10.3103/S0096392509010064
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DOI: https://doi.org/10.3103/S0096392509010064