Abstract
In suspensions of epididymal spermatozoa in vitro at +10°C and +37°C, all nuclei-containing and mitochondria-containing structures (normal spermatozoa, spermatozoa with the bent and coiled tails, complexes of head and neck) are with propidium iodide and rhodamine 123, respectively. Intracellular ATP concentration determined by a bioluminescent method in mitochondria-containing elements of suspension decreases (significantly faster at 37°C than at 10°C) up to a certain unchangeable level (2.5 × 10−8 M/l at 37°C and to 1.6 × 10−8 M/l at 10°C per 106 of mitochondria-containing elements). Mechanisms of spermatozoa destruction are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barrois, B., Perez-Pe, R., Gallego, M., et al., Seminal Plasma Proteins Revert the Cold-Clock Damage on Ram Sperm Membrane, Biol. Reprod., 2000, vol. 63, pp. 1531–1537.
Donnelly, E.T., O’Connell, M., McClure, N., and Lewis, S.E., Differences in Nuclear DNA Fragmentation and Mitochodrial Integrity of Semen and Prepared Human Spermatozoa, Human Reprod., 2000, vol. 15, pp. 1552–1561.
Frundhzyan, V.G., Romanova, N.A., Dement’eva, E.I., Brovko, L.Yu., Ugarova, N.N., et al., Content of ATP in Neutrophils and Whole Blood in Population of Altai Krai Living In Areas with Radionuclide Contamination, Radiatsionnaya Biol. Radioekol., 1997, vol. 37, no. 1, pp. 13–19.
Girotto, S., Ferri, E., Graci, F.D., Cascione, M.L., et al., Direct Bioluminescent ADP and ATP Assay in Platelets and Erythrocytes: Application to Quality Control in Transfusional Centers, Eds. Campbell, A.K., Kircka, L.J., and Stanley, P.I., Proceedings of 6th International Symposium on Biolum. and Chemolum., Bioluminescence and Chemoluminescence, 1990, Cambridge, 1991, pp. 491–494.
Kelso, K.A., Redpath, A., Noble, R.C., Speak, B.K., Lipid and Antioxidant Change in Spermatozoa and Seminal Plasma Throughout the Reproductive Period of Bulls, J. Reprod. Fertil., 1997, vol. 109, pp. 1–6.
Mohammadzadeh, S., Maksudov, G.Yu., Kalistratova, E.N., and Doronin, Yu.K., Morphological Changes in Spermatozoa of Mouse Kept in vitro, Vestn. Mosk. Univ., Ser. Biol., 2007, no. 2, pp. 9–13.
Petruzzi, E., Rabini, R.A., Orlando, C., Staffolany, R., et al., Erythrocyte ATP Content and ATP-Dependent Enzyme Activities on Human Ageing, Eds. Campbell, A.K., Kircka, L.J., and Stanley, P.I., Proceedings of 8th International Symposium on Biolum. and Chemolum, Bioluminescence and Chemoluminescence, Fundamental and Applied Aspects, Cambridge, 1994, pp. 458–461.
Ricci, G., Perticarari, S., Fragonas, E., Giolo, E, et al., Apoptosis in Human Sperm: Its Correlation with Semen Quantity and the Presence of Leukocytes, Human. Reprod., 2002, vol. 17, pp. 2665–2672.
Samuilov, V.D., Oleskin, A.V., and Ligunova, E.M., Programmed Cell Death, Biokhimiya, 2000, vol. 65, no. 8, pp. 1029–1045.
Ugarova, N.N., Bioanalytical Applications of Luciferase of Fireflies, Prikladnaya Biokh. Mikrobiol., 1993, vol. 29, pp. 180–192.
Weil, M., Jacobson, M.D., and Raff, M.C., Are Caspases Involved in the Death of Cell with Transcriptionally Inactive Nucleus? Sperm and Chicken Eryhthrocytes, J. Cell Sci., 1998, vol. 111, pp. 2707–2715.
Weng, S.L., Taylor, S.L., Morshedi, M., Schuffner, A., et al., Caspase Activity and Apoptotic Markers in Ejaculated Human Sperm, Mol. Human Reprod., 2002, vol. 8, pp. 984–991.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S. Mohammadzadeh, G.Yu. Maksudov, V.G. Froundjian, E.N. Kalistratova, N.N. Ugarova, Yu.K. Doronin, 2008, published in Vestnik Moskovskogo Universiteta. Biologiya, 2008, No. 2, pp. 7–10.
About this article
Cite this article
Mohammadzadeh, S., Maksudov, G.Y., Froundjian, V.G. et al. Functional changes of mouse spermatozoa stored in vitro. Moscow Univ. Biol.Sci. Bull. 63, 53–56 (2008). https://doi.org/10.3103/S0096392508020028
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0096392508020028