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Genetic Differentiation of the Round Goby (Neogobius melanostomus) from Certain Localities of the Black and Azov Sea Basin Using Microsatellite Loci

Abstract

Allelic variants were identified using SSR-markers, and locus-specific features of the genetic structure of the round goby (Neogobius melanostomus) populations from five samples of the Black and Azov Sea basin were established. When comparing the values of observed Ho and expected He heterozygosity, it was found that the lowest average value for all analyzed loci was noted in the sample of Lake Yalpug (0.69 and 0.60, respectively), and the highest was in the sample of Zmeyniy Island (0.89 and 0.83). The average polymorphism information content (PIC) ranged from 0.64 for Ame129 locus to 0.68 for Ame133, which indicates a high level of polymorphism of selected markers for this fish species. The phylogenetic analysis showed that the main cluster is formed by representatives of the round goby of the localities Zmeyniy Island and Dzharilgatsky Bay (identity was 0.33) as well as the Dnieper-Bug estuary (0.26). The similarity of the round goby of Lake Yalpug with representatives of the main cluster is 0.23. Pisces of this locality occupy a separate position on the dendrogram, which indicates the specificity of their genetic structure that was formed historically under the influence of abiotic factors of the studied geographical zones during prolonged adaptation.

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Funding

This study was performed at the expense of Ministry of Education and Science obtained for research (basic research) on “Patterns of Differentiation of Gobiidae Populations at the Morphological and Genetic Levels in the Black Sea–Azov Basin of Ukraine” in 2017–2019.

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Correspondence to S. I. Tarasjuk, V. V. Zamorov, O. V. Zaloilo, O. Iu. Bielikova or D. B. Radionov.

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Conflict of interests. The authors declare that they have no conflict of interests.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study was performed in accordance with the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes (Strasbourg, 1986) ETS no. 123.

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Translated by V. Mittova

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Tarasjuk, S.I., Zamorov, V.V., Zaloilo, O.V. et al. Genetic Differentiation of the Round Goby (Neogobius melanostomus) from Certain Localities of the Black and Azov Sea Basin Using Microsatellite Loci. Cytol. Genet. 55, 236–247 (2021). https://doi.org/10.3103/S0095452721030105

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  • DOI: https://doi.org/10.3103/S0095452721030105

Keywords:

  • round goby Neogobius melanostomus
  • microsatellite loci
  • molecular genetic markers
  • genetic structure
  • alleles
  • localities