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Complete Mitochondrial Genome of Bubo bubo (Aves, Strigiformes, Strigidae), a National Protected Wildlife Bird in China


We sequenced the complete mitochondrial genome (mitogenome) of Bubo bubo by the shotgun genome skimming method. The mitogenome of B. bubo is 18,956 bp in length with a base composition of 29.6% A, 22.5% T, 33.8% C and 14.1% G, and consists of 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and two non-coding control regions (CRs). All the protein-coding genes begin with the typical ATG codon except ND3 and ND5, which initiate with ATA. Seven of the 13 PCGs terminate with the codon TAA, and three (ND2, ND4 and COX3) of the remainder end with a single T. Whereas, ND1 and COX1 terminate with a codon AGG and ND6 stops with a codon TAG. The ML phylogenetic trees based on 13 protein-coding genes indicated that Bubo species formed a monophyletic group which was sister to the genus Strix within the same family Strigidae.

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  1. Akbulut, Y., Demiraslan, Y., Aslan, K., et al., The macroanatomy of the sacral plexus and its nerves in Eurasian Eagle Owls (Bubo bubo), Anat. Histol. Embryol., 2016, vol. 45, no. 5, pp. 367–372.

    CAS  Article  Google Scholar 

  2. Bankevich, A., Nurk, S., Antipov, D., et al., SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing, J. Comput. Biol., 2012, vol. 19, no. 5, pp. 455–477.

    CAS  Article  Google Scholar 

  3. Barusic, S., Tutis, V., Cikovic, D., et al., The eagle owl Bubo bubo (Aves: Strigidae) in the Eastern Adriatic (Croatia): the study case of a high-density insular population, Ital. J. Zool., 2016, vol. 83, no. 2, pp. 275–281.

    Article  Google Scholar 

  4. Butchart, S., Symes, A., and Ashpole, J., Bubo bubo, The IUCN Red List of Threatened Species version 2017-3, International Union for Conservation of Nature, 2017.

  5. Kang, H., Ding, P., and Li, B., COI sequence variations and phylogenetic relationships among 27 species of Strigidae Birds, Chin. J. Wildl., 2016, vol. 37, no. 4, pp. 351–356.

    Google Scholar 

  6. Kang, H., Li, B., Ma, X., et al., Evolutionary progression of mitochondrial gene rearrangements and phylogenetic relationships in Strigidae (Strigiformes), Gene, 2018, vol. 674, pp. 8–14.

    CAS  Article  Google Scholar 

  7. Katoh, K. and Standley, D.M., MAFFT multiple sequence alignment software version 7: improvements in performance and usability, Mol. Biol. Evol., 2013, vol. 30, pp. 772–780.

    CAS  Article  Google Scholar 

  8. Leon-Ortega, M., Delgado, M.M., Martinez, J.E., et al., Factors affecting survival in Mediterranean populations of the Eurasian Eagle Owl, Eur. J. Wildl. Res., 2016, vol. 62, vol. 343–351.

  9. Lohse, M., Drechsel, O., Kahlau, S., et al., Organellar genome DRAW—a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets, Nucleic Acids Res., 2013, vol. 41, pp. W575–W581.

    Article  Google Scholar 

  10. Ortego, J. and Espada, F., Ecological factors influencing disease risk in Eagle Owls Bubo bubo, IBIS, 2007, vol. 49, pp. 386–395.

    Article  Google Scholar 

  11. Ortega, M.L., Franco, M.J., Martinez, J.E., et al., Factors influencing territorial occupancy and reproductive success in a Eurasian Eagle-owl (Bubo bubo) population, PLoS One, 2017, vol. 12, no. 4. e0175597.

    Article  Google Scholar 

  12. Stamatakis, A., RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies, Bioinformatics, 2014, vol. 3, no. 9, pp. 1312–1313.

    Article  Google Scholar 

  13. Tian, H.J., Ji, J.W., Yang, S., et al., Complete mitochondrial genome of Eagle Owl (Bubo bubo, Strigiformes; Strigidae) from China, Mitochondrial DNA, 2014, vol. 27, no. 2, pp. 1455–1456.

    Google Scholar 

  14. Voous, K.H., Owls of the Northern Hemisphere, London: Collins, 1988.

    Google Scholar 

  15. Zimmer, E.A. and Wen, J., Using nuclear gene data for plant phylogenetics: progress and prospects II, Next-gen approaches, J. Syst. Evol., 2015, vol. 53, pp. 371–379.

    Article  Google Scholar 

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This study was partially supported by the National Natural Science Foundation of China (31870366, 31170359), Shanxi International Science and Technology Cooperation Project (2018), and the Hundred-Talent Project in Shanxi Province.

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Correspondence to Zhu-Mei Ren.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Ren, ZM., Xu, YJ. Complete Mitochondrial Genome of Bubo bubo (Aves, Strigiformes, Strigidae), a National Protected Wildlife Bird in China. Cytol. Genet. 55, 270–273 (2021).

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  • Bubo bubo
  • Strigidae
  • mitochondrial genome
  • phylogeny