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Effect of Temperature Stress on the Althaea officinalis’s “Hairy” Roots Carrying the Human Interferon α2b Gene

Cytology and Genetics volume 55, pages 207–212 (2021)Cite this article

Abstract

“Hairy” roots obtained through genetic transformation of plants by Agrobacterium rhizogenes, a soil phytopathogen, are valuable producers of important secondary metabolites possessing medicinal properties as well as a useful model system for studying plant responses to impacts of unfriendly environmental conditions. This study compares a postponed response of Althaea officinalis L. “hairy” roots to the impacts of short-term cold- and high-temperature stress factors. The results obtained by the study have shown that “hairy” roots from different A. officinalis lines (individual transformational events) are characterized by different sensitivity to short-term temperature stress impacts, regardless of the transformation vectors or the presence of the human interferon(ifn)-α2b gene. High temperature caused a significant level of growth inhibition in roots of all lines, except those with the highest flavonoid content under the control conditions. On the other hand, a short-term cultivation of “hairy” roots at a low temperature did not cause growth suppression. In parallel with growth inhibition caused by a temperature increase, the activation of flavonoid synthesis, which was probably a response of plants to high temperature as a stress factor, was observed. The study has shown a strong (R2 = 0.78) linear dependence between the antioxidant activity of extracts from “hairy” roots and their flavonoid content. Thus, it is obvious that flavonoids participate in the process of response and adaptation of roots to impacts of high-temperature stress.

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Funding

The study was supported by grant no. 27/01.2020 of the National Research Foundation of Ukraine (NRFU)—Biosynthesis of Recombinant Pharmaceutical Proteins in Plants to Counteract the Spread of Some Infectious Diseases of Viral and Bacterial Origin.

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Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 03143, Kyiv, Ukraine

    N. A. Matvieieva, Y. I. Ratushnyak, V. P. Duplij, A. M. Shakhovsky & M. V. Kuchuk

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  1. N. A. Matvieieva
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  2. Y. I. Ratushnyak
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  3. V. P. Duplij
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  4. A. M. Shakhovsky
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  5. M. V. Kuchuk
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Corresponding author

Correspondence to V. P. Duplij.

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The authors declare that they have no conflict of interests. This article does not contain any investigations carried out by any of the authors with the participation of animals or humans.

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Translated by N. Tarasyuk

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Matvieieva, N.A., Ratushnyak, Y.I., Duplij, V.P. et al. Effect of Temperature Stress on the Althaea officinalis’s “Hairy” Roots Carrying the Human Interferon α2b Gene. Cytol. Genet. 55, 207–212 (2021). https://doi.org/10.3103/S0095452721030051

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  • DOI: https://doi.org/10.3103/S0095452721030051

Keywords:

  • Agrobacterium rhizogenes
  • Althaea officinalis
  • “hairy” roots
  • temperature stress
  • flavonoids
  • antioxidant activity