Abstract—Riboflavin or vitamin B2 is a necessary component for all living organisms since it is the precursor of flavin coenzymes FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide), which are involved in numerous enzymatic reactions. Flavinogenic yeast Candida famata overproduces riboflavin under iron starvation; however, regulation of this process is poorly understood. Regulatory gene SEF1 encoding the transcription activator has been identified. Its deletion blocks yeast’s ability to overproduce riboflavin under iron starvation. It is known that, in the pathogenic flavinogenic yeast C. albicans, Sfu1 (GATA-type transcription factor) represses SEF1. It is demonstrated in this study that deletion of the SEF1 gene in wild type C. famata leads to overproduction of riboflavin.
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The study was partially supported by the grant of the Polish National Science Centre (NCN) Opus UMO-2018/29/B/NZ1/01-497 and the National Academy of Sciences of Ukraine (grant 36-19).
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Translated by V. Mittova
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Petrovska, Y., Lyzak, O., Dmytruk, K. et al. Effect of Gene SFU1 on Riboflavin Synthesis in Flavinogenic Yeast Candida famata . Cytol. Genet. 54, 408–412 (2020). https://doi.org/10.3103/S0095452720050060
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DOI: https://doi.org/10.3103/S0095452720050060