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Comparative Genetic Characteristics of the Russian and Belarusian Populations of Wisent (Bison bonasus), North American Bison (Bison bison) and Cattle (Bos taurus)

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Abstract

A comparative study of the allele pool and the genetic structure of two Russian and one Belarusian populations of the wisent Bison bonasus against the representatives of the species of Bison bison and the genus Bos taurus was carried out. Russian populations were represented by samples of the Oka State Natural Biosphere Reserve (n = 42) and the Prioksko-Terrasny Nature Biosphere Reserve (n = 69), the Belarusian population was represented by samples of the Reserve “Belovezhskaya Pushcha” (n = 42), bison samples (n = 8) and cattle (n = 55) were used as an outgroup. The analysis of the mtDNA D-loop 630 bp fragment polymorphism for the presence of bison and cattle haplotypes, was performed. It was shown that there was a single haplotype for all wisent (Bison bonasus) which was different from the sequences of bison (Bison bison) and cattle. The analysis of population genetic parameters, calculated using 11 microsatellite markers, showed reduced diversity in wisent (Bison bonasus) groups compared to bison (Bison bison) and cattle. The largest number of monomorphic loci as well as the absence of private alleles were found in the group of Belarusian wisent. The analysis of the DJost pairwise genetic distances allowed us to establish clear genetic differentiation of the wisent from the outgroups. This fact was also confirmed by PCA analysis, carried out in the context of population identity and the analysis of population structure which demonstrated some proximity of bison (Bison bison) to wisent (Bison bonasus), which is due to their belonging to the same genus. The data obtained from this study showed differentiation between wisent (Bison bonasus) from bison (Bison bison) and cattle. It can be used in assessing the population genetic parameters of wisent, identifying and eliminating hybrid individuals. It also can be used in developing strategies and measures for the preservation and improvement of the wisent genetic resources.

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ACKNOWLEDGMENTS

The studies were carried out with the financial support of the Russian Foundation for Basic Research, project no. 18-516-00008 and the Belarusian Republican Foundation for Basic Research, project no. B18R-165.

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Authors and Affiliations

  1. Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst, 142132, Dubrovitsy, Russia

    O. V. Kostyunina, A. V. Dotsev, V. V. Volkova, M. S. Fornara, N. A. Akopyan & N. A. Zinovieva

  2. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, 220072, Minsk, Belarus

    M. E. Mikhailova

  3. The Prioksko-Terrasny Nature Biosphere Reserve named after Mikhail Zablotsky, 142200, Danki, Russia

    I. I. Zemlyanko

  4. Mykolayiv National Agrarian University, 54020, Mykolayiv, Ukraine

    A. S. Kramarenko

  5. Institute for Biological Problems of Cryolithozone Siberian Branch of RAS, 677980, Yakutsk, Russia

    I. M. Okhlopkov

  6. Don State Technical University (DSTU), 344000, Rostov-on-Don, Russia

    P. V. Aksenova

  7. Oka State Natural Biosphere Reserve, 391072, Brykin Bor, Russia

    E. L. Tsibizova

  8. World Wildlife Fund, 109240, Moscow, Russia

    P. V. Aksenova & R. A. Mnatsekanov

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  1. O. V. Kostyunina
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  2. M. E. Mikhailova
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  3. A. V. Dotsev
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  4. I. I. Zemlyanko
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  5. V. V. Volkova
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  6. M. S. Fornara
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  7. N. A. Akopyan
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  8. A. S. Kramarenko
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  9. I. M. Okhlopkov
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  10. P. V. Aksenova
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  11. E. L. Tsibizova
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  12. R. A. Mnatsekanov
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  13. N. A. Zinovieva
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Corresponding authors

Correspondence to O. V. Kostyunina, M. E. Mikhailova, A. V. Dotsev, I. I. Zemlyanko, V. V. Volkova, M. S. Fornara, N. A. Akopyan, I. M. Okhlopkov, P. V. Aksenova, E. L. Tsibizova, R. A. Mnatsekanov or N. A. Zinovieva.

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Kostyunina, O.V., Mikhailova, M.E., Dotsev, A.V. et al. Comparative Genetic Characteristics of the Russian and Belarusian Populations of Wisent (Bison bonasus), North American Bison (Bison bison) and Cattle (Bos taurus). Cytol. Genet. 54, 116–123 (2020). https://doi.org/10.3103/S0095452720020085

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