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Expression Levels of Genes Ptgs2 and Tgfb1 in Esophageal Burns and When Introducing Melanin

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Abstract

In this histological study, the degree of esophageal burn was confirmed and the effect of melanin on healing processes, namely, faster periods of recovery of damaged esophageal tissues, was assessed. Higher expression of genes Ptgs2 and Tgfb1 involved in the development of inflammation in blood and esophageal mucosa under conditions of second-degree alkali esophageal burn (AEB 2) was shown. After administration of melanin, the expression levels of genes Ptgs2 and Tgfb1 in blood and esophageal tissues decreased compared to those in the AEB 2 group. It was found that the content of proinflammatory (IL-1β, TNF-α) cytokines in blood and esophageal tissues increased in AEB 2. After administration of melanin, the content of proinflammatory cytokines decreased compared to those of the AEB 2 group. The obtained results demonstrated the anti-inflammatory properties of this compound, showing the prospect of using melanin as a substance contributing to chemical esophageal burn healing.

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Funding

This study did not receive any specific grants from financial institutions in the public, commercial, or noncommercial sectors.

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Authors and Affiliations

  1. Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, 01033, Kyiv, Ukraine

    N. M. Chornenka, Ya. B. Raetska, A. S. Dranitsina, O. O. Kalmikova, N. E. Dzerginskiy, O. M. Savchuk & L. I. Ostapchenko

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  1. N. M. Chornenka
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  2. Ya. B. Raetska
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  3. A. S. Dranitsina
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  4. O. O. Kalmikova
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  6. O. M. Savchuk
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  7. L. I. Ostapchenko
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Correspondence to N. M. Chornenka.

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Conflict of interests. The authors state that they have no conflict of interests.

Statement on the welfare of animals. In this work, we adhered to the general ethical principles of experiments on animals adopted by the First National Congress of Bioethics in Ukraine (September 2001) and with the observance of the international principles of the European Convention for the Protection of Vertebrate Animals used for research and other scientific purposes, other international agreements, and the national legislation in this area.

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Translated by K. Lazarev

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Chornenka, N.M., Raetska, Y.B., Dranitsina, A.S. et al. Expression Levels of Genes Ptgs2 and Tgfb1 in Esophageal Burns and When Introducing Melanin. Cytol. Genet. 53, 367–374 (2019). https://doi.org/10.3103/S0095452719050050

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