Abstract
A multistep in vitro cell selection was carried out on a well-proliferating callus tissue of sugar beet capable of morphogenesis. A 20-day culture filtrate of the fungus Fusarium oxysporum was used as a selective agent. The general features of the behavior of sugar beet callus cells cultivated under stress conditions on increasing concentrations of the culture filtrate, 5, 10, 15, and 20%, have been established. It was found that a low concentration of CF (culture filtrate) of the pathogen (5%) significantly stimulated the process of callusogenesis, while high concentrations (10–20%) had an inhibitory effect. As a result of cell and tissue selection, calluses that retained the ability for normal growth in the presence of sublethal concentrations of the selective agent were selected. By the method of multistep selection, 82 callus lines characterized by resistance to the toxic effects of CF were selected. From selected callus lines, 47 regenerated plants adapted to the soil were obtained. Only 24 plants passed the test for resistance to the fungus Fusarium oxysporum under in vivo conditions.
Similar content being viewed by others
REFERENCES
McGrath, J.M., Townsend, B.J., Sugar Beet, Energy Beet, and Industrial Beet. Industrial Crops. Handbook of Plant Breeding, New York: Springer, 2015, vol. 9. https://doi.org/10.1007/978-1-4939-1447-0_5
Maui, A., Urazaliev, K., and Abekova, A., Diseases of sugar beet in Kazakhstan, in Agricultural Research Updates, New York: Nova Sci. Publ., 2016, vol. 12, no. 9, pp. 143–171.
Podvigina, O.A., Theoretical substantiation and approaches in using biotechnological methods in sugar beat breeding, Doctoral (Agricult.) Dissertation, Voronezh: Voronezh. Agrar. Univ., 2003. OD, 71:04-6/37-2
Soboleva, G.V., Influence of osmotic stress on processes of growth and morphogenesis in long-term callus cultures of pea (Pisum sativum L.), Sci. Prod. J. “Grain Legumes and Cereal Crops”, 2013, no. 1(5), pp. 8–15.
Rao, S. and Sandhya, H., In Vitro Selection of Disease-Resistant Plants. Plant Tissue Culture: Propagation, Conservation and Crop Improvement, Singapore: Springer, 2016. https://doi.org/10.1007/978-981-10-1917-3_17
Binarova, P., Nedelnik, J., Fellner, M., and Nedbalkova, B., Selection for resistance to filtrates of Fusarium spp. in embryogenic cell suspension culture of Medicago sativa L., Plant Cell Tiss. Organ Cult., 1990, vol. 22, no. 3, pp. 191–196. https://doi.org/10.1007/BF00033635
Chawla, H.S. and Wenzel, G., In vitro selection for fusaric acid resistant barley plants, Plant Breed., 1987, vol. 99, pp. 159–163. https://doi.org/10.1111/j.1439-0523.1987.tb01166.x
Hashem, E.A., Abdalla, H.E., Hussein, Y.A., and Abd-Elnabi, M.A., In vitro selection of soybean callus resistant to Fusarium oxysporum metabolites, in Proceedings Third Environment Conference, Faculty of Sci., Zagazig Univ., 2008, pp. 1–19.
Kasem, Z.A., Mesterházy, B., Bartyk, T., and Sági, F., In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. II. Culture filtrate technique and inheritance of Fusarium-resistance in the somaclones, Euphytica, 1996, vol. 91, no. 3, pp. 341–349. https://doi.org/10.1007/BF00033096
Khan, I.A., Alam, S.S., and Jabbar, A., Purification of phytotoxin from culture filtrates of Fusarium oxysporum f. sp. ciceris and its biological effects on chickpea, Pak. J. Bot., 2004, vol. 36, pp. 871–880.
Rutherford, R., Stuart, Snyman., Sandy, J., and Watt, M.P., In vitro generation of somaclonal variant plants of sugarcane for tolerance to Fusarium sacchari, Plant Cell Rep., 2013, vol. 32, no. 2, pp. 249–262. https://doi.org/10.1007/s00299-012-1359-0
Purwati, R.D. and Harran, S., Sudarsono. In vitro selection of abaca for resistance to Fusarium oxysporum f. sp. cubense, Hayati J. Biosci., 2007, vol. 14, no. 2, pp. 65–70. https://doi.org/10.4308/hjb.14.2.65
Esmaiell, N.M., Al-Doss, A.A., and Barakat, M.N., In vitro selection for resistance to Fusarium oxysporum f. sp. dianthi and detection of genetic polymorphism via RAPD analysis in carnation, Med. Plants Res., vol. 6, no. 23, pp. 3997–4004. https://doi.org/10.5897/JMPR12.150
Chen, W. and Swart, W.J., The in vitro phytotoxicity of culture filtrates of Fusarium oxysporum to five genotypes of Amaranthus hybridus, Euphytica, 2002, vol. 127, pp. 61–67.
Urazaliev, K., Abekova, A., Bazylova, T., Bersimbaeva, G., Daniyarova, A., and Massonichich-Shotunova, R., Somaclonal variation of sugar beet resistant to pathogenic root rot Fusarium oxysporum var. orthoceras, Genetika, 2013, vol. 45, pp. 629–640. https://doi.org/10.2298/gensr1303629u
Kalashnikova, E.A., Cell breeding of plants for resistance to fungal diseases, Doctoral (Biol.) Dissertation, Moscow: Timiryazev Moscow Agricultural Academy, 2003. OD, 71:04-3/116.
Murashige, T. and Skoog, F., A revised medium for rapid growth and bio assays with tobacco culture, Physiol. Plantarum, 1962, vol. 15, pp. 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Xu, Q.L., Xie, Y.H., Ru, H., Hu, X., Wang, Ch.Y., and Wang, X.Yu., Efficient plant regeneration in vitro from red leaf beet via organogenesis, Russ. J. Plant Physiol., 2009, vol. 56, no. 4, pp. 546–550. https://doi.org/10.1134/S1021443709040153
Walker, J.C., Plant Pathology, New York: McGraw-Hill Book, 1969, 3rd ed.
Freytag, A.H., Anan, S.C., Rao-Ardelli, A.P., and Owens, L.D., An improved medium for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro, Plant Cell Rep., 1988, vol. 7, pp. 30–34. https://doi.org/10.1007/BF00272972
Golovko, A.E., Dovzhenko, A.A., and Gleba, Yu.Yu., Genetic transformation of sugar beet: the evolution of attitudes and methodological approaches, Cytol. Genet., 2005, vol. 39, no. 3, pp. 30–36.
Hisano, H., Kimoto, Y., Hayakawa, H., Takeichi, J., Domae, T., Hashimoto, R., Abe, J., Asano, S., Kanazawa, A., and Shimamoto, Y., High frequency Agrobacterium mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritime, Plant Cell Rep., 2004, vol. 22, pp. 910–918. https://doi.org/10.1007/s00299-004-0773-3
Dovzhenko, A. and Koop, H., Sugar beet (Beta vulgaris L.): shoot regeneration from callus and callus protoplasts, Planta, 2003, vol. 217, pp. 374–381. https://doi.org/10.1007/s00425-003-1006
Mishutkina, Ya.V. and Gaponenko, A.K., Sugar beet (Beta vulgaris L.) morphogenesis in vitro: effects of phytohormone type and concentration in the culture medium, type of explants, and plant genotype on shoot regeneration frequency, Russ. J. Genet., 2006, vol. 42, no. 2, pp. 150–157. https://doi.org/10.1134/S1022795406020086
Funding
The study was supported by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan within the budget program 217 “Development of Science,” subprogram 102 “Grant Financing of Scientific Research” to project no. 4785/GF4.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Additional information
Translated by V. Mittova
About this article
Cite this article
Yerzhebayeva, R.S., Abekova, A.M., Bersimbaeva, G.H. et al. In Vitro Cell Selection of Sugar Beet for Resistance to Culture Filtrate of the Fungus Fusarium oxysporum. Cytol. Genet. 53, 307–314 (2019). https://doi.org/10.3103/S0095452719040042
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0095452719040042