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Morphometric and ISSR-Analysis of Local Populations of Geranium molle L. from the Southern Coast of the Caspian Sea

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Abstract

Geranium molle is known as Dovefoot Geranium or Awnless Geranium. Dovefoot Geranium is a low-growing herb with pink flowers and sharply toothed leaves. Dovefoot Geranium is native to Eurasia and has been introduced to many habitats of the world. This species is very similar to G. robertianum but its palmate-like leaves and bilobed petals show differences. This plant is considered to be anodyne, astringent and vulnerary. We have no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Therefore, due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 132 randomly collected plants from 18 geographical populations in 4 provinces. Genetic diversity parameters were determined in these populations. STRUCTURE analysis and K-Means clustering identified 14 gene pools in the country and revealed isolation by distance among the studied populations. The Mantel test showed correlation between genetic and geographical distance. AMOVA revealed a significant genetic difference among populations and showed that 40% of total genetic variation was due to within-population diversity. The consensus tree of both molecular and morphological data identified divergent populations. These data may be used in future breeding and conservation of this important medicinal plant in the country.

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References

  1. Chen, X.Y., Effects of fragmentation on genetic structure of plant populations and implications for the biodiversity conservation, Acta. Ecol. Sin., 2000, vol. 20, pp. 884–892.

    Google Scholar 

  2. Ellis, J.R. and Burke, J.M., EST-SSRs as a resource for population genetic analyses, Heredity, 2007, vol. 99, no. 2, pp. 125–132.

    Article  PubMed  CAS  Google Scholar 

  3. Aedo, C., Revision of Geranium subgenus Erodioidea (Geraniaceae), Syst. Bot. Monogr., 1996, vol. 49, pp. 1–104.

    Article  Google Scholar 

  4. Aedo, C., Garmendia, F.M., and Pando, F., World checklist of Geranium L. (Geraniaceae), Anales. Jard. Bot. Madrid, 1998, vol. 56, pp. 211–252.

    Google Scholar 

  5. Aedo, C., Aldasoro, J.J., and Navarro, C., Taxonomic revision of Geranium L., sections Divaricata Rouy and Batrachioidea W.D.J. Koch (Geraniaceae), Ann. Miss. Bot. Gard., 1998, vol. 85, pp. 594–630.

    Article  Google Scholar 

  6. Mabberley, D.J., Mabberley’s Plant Book: A Portable Dictionary of Plants, Their Classification and Uses, Cambridge: Cambridge Univ. Press, 2009.

    Google Scholar 

  7. Yeo, P.F., Fruit-discharge-type in Geranium (Geraniaceae): its use in classification and its evolutionary implications, Bot. J. Linn. Soc., 1984, vol. 89, no. 1, pp. 1–36. org/doi 10.1111/j.1095-8339.1984.tb00998.x

    Article  Google Scholar 

  8. Janighorban, M., Flora of Iran, Geraniaceae, The Research Institute of Forests and Rangelands, 2009, vol. 62.

  9. Onsori, S., Salimpour, F., and Mazooji, A., The new record of Geranium linearilobium Dc. based on anatomy and micromorphological study of pollen and seed, in Iran, J. Plant Environ. Physiol., 2010, vol. 5, no. 3, pp. 21–30.

    Google Scholar 

  10. Schonbeck-Temesy, E., Geraniaceae, in Flora Iranica, Rechinger, K.H., Ed., Graz, Austria: Akademische Druck, 1970, vol. 69, pp. 30–58.

  11. Yeo, P.F., The classification of Geraniaceae, in Proceeding of the International Geraniaceae Symposium, Univ. of Stellenbosch, RSA, Vorster, P., Ed., 1990, pp. 1–22.

    Google Scholar 

  12. Price, R.A. and Palmer, J.D., Phylogenetic relationships of the Geraniaceae and Geraniales from rbcL sequence comparisons, Ann. Missouri Bot. Gard., 1993, vol. 80, no. 3, pp. 661–671.

    Article  Google Scholar 

  13. Chen, C. and Wang, C., Geranium molle L. (Geraniaceae), a newly naturalized plant in Taiwan, Coll. Res., 2005, vol. 18, pp. 11–14.

    Google Scholar 

  14. Pilorge, E. and Mircovich, C., Weed control strategies using GMO herbicide tolerant oilseed rape, in Proc. 10th Int. Rapes. Congress, Canberra, Australia, 1999.

    Google Scholar 

  15. Roberts, H.A. and Boddrell, J.E., Seed survival and seasonal emergence in some species of Geranium, Ranunculus and Rumex (abstract), Ann. Appl. Biol., 1985, vol. 107, no. 2, pp. 231–238. org/doi 10.1111/j.1744-7348.1985.tb01566.x

    Article  Google Scholar 

  16. Martin, J.P. and Sanchez-Yelamo, M.D., Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers, Theor. Appl. Genet., 2000, vol. 101, no. 8, pp. 1234–1241. doi 10.1007/s001220051602

    Article  CAS  Google Scholar 

  17. Manica-Cattani, M.F., Zacaria, J., Pauletti, G., Atti-Serafini, L., and Echeverrigaray, S., Genetic variation among South Brazilian accessions of Lippia alba Mill. (Verbenaceae) detected by ISSR and RAPD markers, Braz. J. Biol., 2009, vol. 69, no. 2, pp. 375–80.

    Article  PubMed  CAS  Google Scholar 

  18. Azizi, N., Sheidai, M., Mozafarian, V., and Noormohammadi, Z., Genetic, cytogenetic and morphological diversity in Helicrysum leucocephalum (Asteraceae) populations, Biologia, 2014, vol. 69, no. 5, pp. 566–573.

    Article  Google Scholar 

  19. Sheidai, M., Seif, E., Nouroozi, M., and Noormohammadi, Z., Cytogenetic and molecular diversity of Cirsium arvense (Asteraceae) populations in Iran, J. Jpn. Bot., 2012, vol. 87, pp. 193–205.

    Google Scholar 

  20. Sheidai, M., Ziaee, S., Farahani, F., Talebi, S.M., Noormohammadi, Z., and Hasheminejad-Ahangarani Farahani, Y., Intra-specific genetic and morphological diversity in Linum album (Linaceae), Biologia, 2014, vol. 69, no. 1, pp. 32–39. org/doi 10.2478/s11756-013-0281-4

    Article  CAS  Google Scholar 

  21. Zohary, M., Flora Palaestina. Platanaceae to Umbelliferae, Jerusalem, Israel: The Israel Academy of Sciences and Humanities, 1972.

    Google Scholar 

  22. Davis, P.H., Geraniacea, in Flora of Turkey and East Aegean Islands, 1965, vol. 2, pp. 450–488.

    Google Scholar 

  23. Podani, J., Introduction to the Exploration of Multivariate Data, Leiden: Backhuyes Publ., 2000.

    Google Scholar 

  24. Hammer, Q., Harper, D.A.T., and Ryan, P.D., PAST: Paleontological Statistics software package for education and data analysis, Palaeontol. Electr., 2001, vol. 4, no. 1, pp. 1–9.

    Google Scholar 

  25. Weising, K., Nybom, H., Wolff, K., and Kahl, G., DNA Fingerprinting in Plants. Principles, Methods, and Applications, 2nd ed., Boca Raton: CRC Press, 2005.

    Book  Google Scholar 

  26. Freeland, J.R., Kirk, H., and Peterson, S.D., Molecular Ecology, 2nd ed., UK: Wiley-Blackwell, 2011.

    Book  Google Scholar 

  27. Huson, D.H. and Bryant, D., Application of phylogenetic networks in evolutionary studies, Mol. Biol. Evol., 2006, vol. 23, no. 2, pp. 254–267. doi 10.1093/molbev/msj03

    Article  PubMed  CAS  Google Scholar 

  28. Peakall, R. and Smouse, P.E., GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research, Mol. Ecol. Notes, 2006, vol. 6, pp. 288–295.

    Article  Google Scholar 

  29. Meirmans, P.G. and Van Tienderen, P.H., GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms, Mol. Ecol. Notes, 2004, vol. 4, pp. 792–794.

    Article  Google Scholar 

  30. Hedrick, P.W., A standardized genetic differentiation measure, Evolution, 2005, vol. 59, no. 8, pp. 1633–1638.

    Article  PubMed  CAS  Google Scholar 

  31. Jost, L., GST and its relatives do not measure differentiation, Mol. Ecol., 2008, vol. 17, pp. 4015–4026.

    Article  PubMed  Google Scholar 

  32. Pritchard, J.K., Stephens, M., and Donnelly, P., Inference of population structure using multilocus genotype data, Genetics, 2000, vol. 155, no. 2, pp. 945–959.

    PubMed  PubMed Central  CAS  Google Scholar 

  33. Falush, D., Stephens, M., and Pritchard, J.K., Inference of population structure using multilocus genotype data: dominant markers and null alleles, Mol. Ecol. Notes, 2007, vol. 7, no. 4, pp. 574–578.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  34. Evanno, G., Regnaut, S., and Goudet, J., Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study, Mol. Ecol., 2005, vol. 14, no. 8, pp. 2611–2620. doi 10.1111/j.1365-294X.2005.02553.x

    Article  PubMed  CAS  Google Scholar 

  35. Meirmans, P.G., AMOVA-based clustering of population genetic data, J. Heredity, 2012, vol. 103, no. 5, pp. 744–750. doi 10.1093/jhered/ess047

    Article  Google Scholar 

  36. Jolivet, C. and Bernasconi, G., Molecular and quantitative genetic differentiation in European populations of Silene latifolia (Caryophyllaceae), Ann. Bot., 2007, vol. 100, no. 1, pp. 119–127. doi 10.1093/aob/mcm088

    Article  PubMed  PubMed Central  Google Scholar 

  37. Baker, H.G., Geranium purpureum Vill. and G. robertianum L. in the British flora. II: Geranium purpureum, Watsonia, 1955, vol. 3, pp. 160–167.

    Google Scholar 

  38. Heuertz, M., Hausman, J.F., Hardy, O.J., Vendramin, G.G., Franscaria-Lacoste, N., and Vekemans, X., Nuclear microsatellites reveal contrasting patterns of genetic structure between western and southeastern European populations of the common ash (Fraxinus excelsior L.), Evolution, 2004, vol. 58, pp. 976–988.

    PubMed  Google Scholar 

  39. Lawton, J.H., Range, population abundance and conservation, Trends Ecol. Evol., 1993, vol. 8, no. 11, pp. 409–413.

    Article  PubMed  CAS  Google Scholar 

  40. Hampe, A. and Petit, R.J., Conserving biodiversity under climate change: the rear edge matters, Ecol. Lett., 2005, vol. 8, no. 5, pp. 461–467.

    Article  PubMed  Google Scholar 

  41. Caliskan, M., Genetic Diversity in Plants, InTech, Chapters published under CC BY 3.0 license, 2012. doi 10.5772/2640

    Book  Google Scholar 

  42. Esfandani, S., Sheidai, M., and Noormohammadi, Z., Species delimitation and population structure analysis in Geranium subg. Geranium (Geraniaceae) with the use of ISSR molecular markers, Biologia, 2017, vol. 72, no. 7.

  43. Hutchison, D.W. and Templeton, A.R., Correlation of pairwise genetic and geographic distance measures: inferring the relative influences of gene flow and drift on the distribution of genetic variability, Evolution, 1999, vol. 53, no. 6, pp. 1898–1914. doi 10.1111/j.1558-5646.1999.tb04571.x

    Article  PubMed  Google Scholar 

  44. Medrano, M. and Herrera, C.M., Geographical structuring of genetic diversity across the whole distribution range of Narcissus longispathus, a habitat-specialist, Mediterranean narrow endemic, Annal. Bot., 2008, vol. 102, no. 2, pp. 183–194. doi 10.1093/aob/mcn086

    Article  Google Scholar 

  45. Slatkin, M., Isolation by distance in equilibrium and non-equilibrium populations. Evolution, 1993, vol. 47, no. 1, pp. 264–279.

    Article  PubMed  Google Scholar 

  46. Hufford, K.M. and Mazer, S.J., Plant ecotypes: genetic differentiation in an age of ecological restoration, Trends Ecol. Evol., 2003, vol. 18, no. 3, pp. 147–55. doi 10.1016/S0169-5347(03)00002-8

    Article  Google Scholar 

  47. Gibson, D.J., Grasses and Grassland Ecology, Oxford, UK: Oxford Univ. Press, 2009.

    Google Scholar 

  48. Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M., and Noormohammadi, Z., Genetic diversity and morphological variability in Geranium purpureum Vill. (Geraniaceae) of Iran, Genetika, 2017, vol. 49, no. 2, pp. 543–557. doi 10.2298/GENSR1702543B

    Article  Google Scholar 

  49. Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M., and Noormohammadi, Z., Species delimitation in Geranium sect. Batrachioidea: morphological and molecular, Act. Bot. Hung., 2017, vol. 59, nos. 3–4, pp. 319–334. org/doi 10.1556/034.59.2017.3-4.3

    Article  Google Scholar 

  50. Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M., and Noormohammadi, Z., Genetic and morphological diversity in Geranium dissectum (Sec. Dissecta, Geraniaceae) populations, Biologia, 2017, vol. 72, no. 10, pp. 1–10. doi 10.1515/biolog-2017-0124

    Article  Google Scholar 

  51. Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M., and Noormohammadi, Z., Analysis of genetic diversity in Geranium robertianum by ISSR markers, Phytol. Balcanica, 2017, vol. 23, no. 2, pp. 157–166.

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

    S. Esfandani-Bozchaloyi & M. Sheidai

  2. Department of Plant Sciences, Faculty of Biological Science, Alzahra University, Tehran, Iran

    M. Keshavarzi

  3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Z. Noormohammadi

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  1. S. Esfandani-Bozchaloyi
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  2. M. Sheidai
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  3. M. Keshavarzi
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  4. Z. Noormohammadi
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Correspondence to S. Esfandani-Bozchaloyi.

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Esfandani-Bozchaloyi, S., Sheidai, M., Keshavarzi, M. et al. Morphometric and ISSR-Analysis of Local Populations of Geranium molle L. from the Southern Coast of the Caspian Sea. Cytol. Genet. 52, 309–321 (2018). https://doi.org/10.3103/S0095452718040102

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