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Induction of Arabidopsis thaliana Resistance to Pathogenic Bacteria by Lipopolysaccharide and Salicylic Acid

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An Erratum to this article was published on 24 July 2018

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Abstract

The effects of combined treatment with an elicitor (lipopolysaccharide) and a signaling molecule (salicylic acid) on the disease resistance of wild-type (Col-0) and mutant Arabidopsis thaliana L. plants have been compared. The mutant lines used were jin1 (with impaired jasmonate signaling), npr1 (lacking expression of pathogen-dependent PR genes), and NahG (expressing an active bacterial salicylate hydroxylase transgene). The lipopolysaccharide was isolated from a saprophytic strain (8614) of Pseudomonas aeruginosa bacteria. Treatment of A. thaliana seeds with a composite preparation (lipopolysaccharide and salicylic acid–SA) increased the resistance of seedlings to a subsequent infection by the pathogenic 9096 strain of P. aeruginosa bacteria. The protective effect was more pronounced in jin1 mutant seedlings, which was indicative of the possible compensation of jasmonate signaling impairment due to activation of the SA-dependent signaling pathway. We concluded that a preparation composed of an elicitor and a signaling molecule could affect regulatory mechanism functioning in a plant cell and, in particular, compensate for the absence of a certain signaling pathway by activating another.

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  • 24 July 2018

    Page 170, right column, the first sentence of the last paragraph should read:

References

  1. Dyakov, Yu.T., Population Biology of Phytopathogenic Fungi, Moscow: Muravei, 1998.

    Google Scholar 

  2. Dmitriev, A.P., Signalling molecules for activation of plant defense reactions in response to biotic stress, Russ. J. Plant Physiol., 2003, vol. 50, no. 3, pp. 1–10.

    Article  Google Scholar 

  3. Ozeretskovskaya, O.L., Vasyukova, N.I., Panina, Ya.S., and Chalenko, G.I., Effect of immunomodulators on potato resistance and susceptibility to Phytophthora infestans, Russ. J. Plant Physiol., 2006, vol. 53, no. 4, pp. 488–494.

    Article  CAS  Google Scholar 

  4. Polyakovsky, S.A., Kravchuk, Zh.N., and Dmitriev, A.P., The mechanisms of action of plant resistance inductors by the example of Allium cepa, Tsitol. Genet., 2008, vol. 42, no. 6, pp. 8–12.

    Google Scholar 

  5. Zhuk, I.V., Lisova, G.M., Dovgal, Z.M., and Dmitriev, A.P., The induction of Triticum aestivum L. tolerance to Septoria tritici by oxalic acid, Modern Phytomorphology, 2014, vol. 6, pp. 105–108.

    Google Scholar 

  6. Tyuterev, S.L., Ecologically safe inducers of plant resistance to diseases and physiological stresses, Plant Protection News, 2015, vol. 1, no. 83, pp. 3–13.

    Google Scholar 

  7. Dmitriev, A.P., Kovbasenko, R.V., Avdeyeva, L.V., Lapa, S.V., and Kovbasenko, V.M., Signal Systems of Plants and Formation of Resistance to Biotic Stress, Kiev: Phoenix, 2015.

    Google Scholar 

  8. Dow, M., Newman, M.A., and von Roepenack, E., The induction and modulation of plant defense responses by bacterial lipopolysaccharides, Annu. Rev. Phytopathol., 2000, vol. 38, pp. 241–261.

    Article  PubMed  CAS  Google Scholar 

  9. Newman, M.A., Dow, J.M., Molinaro, A., and Parrilli, M., Priming, induction and modulation of plant defense responses by bacterial lipopolysaccharides, J. Endotoxin Res., 2007, vol. 13, no. 2, pp. 68–79.

    Article  CAS  Google Scholar 

  10. Mishina, T.E. and Zeier, J., Pathogen-associated molecular pattern recognition rather than development of tissue necrosis contributes to bacterial induction of systemic acquired resistance in Arabidopsis, Plant J., 2007, vol. 50, no. 3, pp. 500–513.

    Article  PubMed  CAS  Google Scholar 

  11. Caarls, L., Pieterse, C.M., and Van Wees, S.C., How salicylic acid takes transcriptional control over jasmonic acid signaling, Front. Plant Sci., 2015, vol. 6, p. 170.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Thaler, J.S., Humphrey, P.T., and Whiteman, N.K., Evolution of jasmonate and salicylate signal crosstalk, Trends Plant Sci., 2012, vol. 17, no. 5, pp. 260–270.

    Article  PubMed  CAS  Google Scholar 

  13. Loake, G. and Grant, M., Salicylic acid in plant defense—the players and protagonists, Curr. Opin. Plant Biol., 2007, vol. 10, no. 5, pp. 466–472.

    Article  PubMed  CAS  Google Scholar 

  14. Shilina, J.V., Guscha, M.I., Molozhava, O.S., Litvinov, S.V., and Dmitriev, A.P., Role of salicylate and jasmonate signaling in lipopolysaccharide-induced resistance of Arabidopsis thaliana to the phytopathogenic strain of Pseudomonas aeruginosa IMB 9096, Visn. Ukr. Tov. Genet. Sel., 2015, vol. 13, no. 1, pp. 100–104.

    Google Scholar 

  15. Zhuk, I.V., Lisova, G.M., and Dmitriev, A.P., Effects of oxalic acid and sodium nitroprusside on productivity and resistance of winter wheat to Septoria leaf blotch and leaf rust infections, Visn. Kharkiv. Nat. Univ. Ser. Biol., 2017, vol. 41, no. 2, pp. 68–77.

    Google Scholar 

  16. Spoel, S.H., Koornneef, A., Claessens, S.M., Korzelius, J.P., Van Pelt, J.A., Mueller, M.J., Buchala, A.J., Metraux, J.P., Brown, R., Kazan, K., Van Loon, L.C., Dong, X., and Pieterse, C.M., NPR1 modulates crosstalk between salicylate-and jasmonate-dependent defense pathways through a novel function in the cytosol, Plant Cell, 2003, vol. 15, no. 3, pp. 760–770.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  17. Doares, S.H., Syrovets, T., Weller, E.W., and Ryan, C.A., Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway, Proc. Natl. Acad. Sci. U. S. A., 1995, vol. 92, no. 10, pp. 4095–4098.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  18. Jia, X., Meng, Q., Zeng, H., Wang, W., and Yin, H., Chitosan oligosaccharide induces resistance to tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway, Sci. Rep., 2016, vol. 6. doi 10.1038/srep26144

  19. Ryabchinskaya, T.A., Bobreshova, I.Yu., Kharchenko, G.L., and Sarantseva, N.A., Remote aftereffect of treatment with phytoactivators for subsequent reproduction of spring barley, in Modern Immunological Studies: Materials of Scientific-Practica. Conf., Bolsye Vyazemy, 2009, pp. 173–179.

    Google Scholar 

  20. Lykova, N.A., The Effect of Preheating. Environmental Aftereffects, St. Petersburg: Nauka, 2009.

    Google Scholar 

  21. Guscha, M.I., Shilina, J.V., and Dmitriev, A.P., Transgenerational transmission of adaptive effects to UV-B irradiation in Arabidopsis thaliana plants, in Vii Congress on Radiation Research, Abstracts of Reports, Moscow, 2014, pp. 207–208.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv, 03680, Ukraine

    J. V. Shilina, M. I. Gushcha, S. V. Litvinov & A. P. Dmitriev

  2. Institute of Biology, Taras Shevchenko National University, Kyiv, 03127, Ukraine

    O. S. Molozhava

Authors
  1. J. V. Shilina
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  2. M. I. Gushcha
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  3. O. S. Molozhava
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  4. S. V. Litvinov
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  5. A. P. Dmitriev
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Corresponding author

Correspondence to J. V. Shilina.

Additional information

Original Ukrainian Text © J.V. Shilina, M.I. Gushcha, O.S. Molozhava, S.V. Litvinov, A.P. Dmitriev, 2018, published in Tsitologiya i Genetika, 2018, Vol. 52, No. 3, pp. 3–8.

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Shilina, J.V., Gushcha, M.I., Molozhava, O.S. et al. Induction of Arabidopsis thaliana Resistance to Pathogenic Bacteria by Lipopolysaccharide and Salicylic Acid. Cytol. Genet. 52, 169–173 (2018). https://doi.org/10.3103/S0095452718030118

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  • DOI: https://doi.org/10.3103/S0095452718030118

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