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Cytology and Genetics

, Volume 52, Issue 1, pp 11–20 | Cite as

Comparison of alleles at the Gli-1 loci of common wheat by means of two-dimensional electrophoresis of gliadin and RFLP analysis

  • E. V. MetakovskyEmail author
  • V. A. Melnik
  • P. Vaccino
  • M. Rodriguez-Quijano
Article

Abstract

Allelic variants of the Gli-1 locus is known to control groups (blocks) of gliadin polypeptides (gliadins). Some allelic variants of blocks that differ in the electrophoretic (acid gel) mobility (EM) of only one gliadin of the block were compared using two-dimensional electrophoresis (SDS-PAGE) and the RFLP procedure. It was found that, in these pairs of similar alleles (Gli-B1f, Gli-B1s, and Gli-D1a as compared with Gli-B1e, Gli-B1n, and Gli-D1c, respectively), faster γ-gliadin had smaller molecular weight (MW). Alleles at the Gli-A1 locus (Gli-A1j, Gli-A1i, Gli-A1a, Gli-A1k, and Gli-A1f) differ in the EM of the γ-gliadin so that Gli-A1j controls the slowest γ-gliadin and Gli-A1f controls the fastest one. We found that, in this order of alleles, faster γ-gliadin always had smaller MW. It was suggested that similar alleles might arise from one another by spontaneous mutations changing the number of repeating sequences or length of the polyglutamine domain present in the γ-gliadin gene thereby influencing MW and EM of encoding polypeptide. Other mechanisms of the mutational appearance of new alleles were found earlier by comparison of allele pairs: Gli-D1a and Gli-D1k (gene silencing) and Gli-D1b and Gli-D1d (gene amplification). We discovered contrasting families of alleles at the Gli-B1 and at the Gli-D1 loci and also two variants of apparently the same allele Gli-D1a that differed in the number of encoded ω-gliadins. Families of alleles at one locus of T. aestivum might inherit from different genotypes of corresponding diploid donor, as we suggested earlier.

Keywords

gliadin alleles mutations common wheat 

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Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • E. V. Metakovsky
    • 1
    Email author
  • V. A. Melnik
    • 1
  • P. Vaccino
    • 2
  • M. Rodriguez-Quijano
    • 1
  1. 1.Department of GeneticsPolytechnic University of MadridMadridSpain
  2. 2.Experimental Institute for Cereal ResearchSant’Angelo LodigianoItaly

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