Abstract
Samples of trees and seed embryos of five Pinus kochiana Klotzsch ex Koch populations in Crimea were comparatively studied at 12 allozymic loci for its genetic variability and mating system. It has been shown that the embryos of seeds reproduce the allelic diversity of maternal plants, but the level of their observed heterozygosity (Ho) is significantly lower, i.e., 0.286 vs. 0.189, respectively. Unlike maternal trees, embryos are characterized by a significant divergence between the observed distribution of genotypes and the theoretically expected one according to the Hardy–Weinberg law for the majority of analyzed loci. The frequency of cross pollination in populations varied from 0.384 to 0.673 according to single-locus (t s) estimates and from 0.639 to 0.841 according to multilocus (t m) estimates.
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References
Altukhov, Y.P., The Dynamics of Population Gene Pools Under Anthropogenic Influences, Moscow: Nauka, 2004.
Didukh, Ya.P., The Vegetation Cover of the Crimean Mountains (Structure, Dynamics, and Evolution of the Security), Kyiv: Naukova dumka, 1992.
Korshikov, I.I., Podgornyi, D.Y., Kalafat, L.A., and Pirko, Ya.V., Taxonomic status of Pinus kochiana Klotzsch ex Koch from Mountain Crimea on data comparative population and genetic research to Pinus sylvestris L., Ukr. Bot. J., 2011, vol. 68, no. 1, pp. 133–142.
Delgado, P., Cuenca, A., Escalante, A.E., Molina-Freaner, F., and Pinero, D., Comparative genetic structure in pines: evolutionary and conservation consequences, Rev. Chil. Hist. Nat., 2002, vol. 75, no. 1, pp. 27–37.
Politov, D.V., Genetics of populations and evolutionary relationships of pine species (Family Pinaceae) of northern Eurasia, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow, 2007.
Davis, B.J., Disk electrophoresis. 2. Methods and application to human serum proteins, Ann. N.Y. Acad. Sci., 1964, vol. 121, pp. 404–427.
Nei, M., Genetic distance between populations, Am. Nat., 1972, vol. 106, no. 949, pp. 283–292.
Swofford, D.L. and Selander, R.B., BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematic, J. Hered., 1981, vol. 72, no. 4, pp. 281–283.
Peakall, R. and Smouse, P.E., GenAlex 6: Genetic Analysis in Excel. Population genetic software for teaching and research, Mol. Ecol. Notes, 2006, vol. 6, no. 1, pp. 288–295.
Ritland, K., Extensions of models for the estimation of mating systems using n independent loci, Heredity, 2002, vol. 88, pp. 221–228.
Bower, A.D. and Aitken, S.N., Mating system and inbreeding depression in whitebark pine (Pinus albicaulis Engelm.), Tree Genet. Genom., 2007, vol. 3, no. 4, pp. 379–388.
Korshikov, I.I., Pirko, N.N., Mudrik, E.A., and Pirko, Ya.V., Maintenance of genetic structure in progenies of marginal mountainous and steppe populations of three species of Pinacea Lindl. Family in Ukraine, Silvae Genet., 2007, vol. 56, no. 1, pp. 1–10.
Belokon, M.M., Belokon, Yu.S., and Politov, D.V., The mating system and the dynamics of allozyme heterozygosity in Pinus pumila (Pall.) Regel. populations, Conifers Boreal Zone, 2010, vol. 27, nos. 1–2, pp. 13–18.
Ledig, F.T., Capo-Arteaga, M.A., Hodgskiss, P.D., Sbay, H., Flores-Lopez, C., Conkle, M.T., and Bermejo-Velazquez, B., Genetic diversity and the mating system of a rare Mexican pinon, Pinus pinceana, and a comparison with Pinus maximartinezii (Pinaceae), Am. J. Bot., 2001, vol. 88, no. 11, pp. 1977–1987.
Fady, B., Forest, I., Hochu, I., Ribiollet, A., de Beaulieu, J.-L., and Pastuszka, P., Genetic differentiation in Abies alba Mill. populations from southeastern France, Forest Genet., 1999, vol. 6, no. 3, pp. 129–138.
Ledig, F.T., Hodgskiss, P.D., and Johnson, D.R., Genic diversity, genetic structure, and mating system of Brewer spruce (Pinaceae), a relict of the Arcto-Tertiary forest, Am. J. Bot., 2005, vol. 92, no. 12, pp. 1975–1986.
Muona, O., Yazdani, R., and Rudin, D., Genetic change between life stages in Pinus sylvestris: allozyme variation in seeds and planted seedlings, Silvae Genet., 1987, vol. 36, no. 1, pp. 39–42.
Yazdani, R., Muona, O., Rudin, D., and Szmidt, A.E., Genetic structure of Pinus sylvestris L. seed-tree stand and naturally regenerated understorey, Forest Sci., 1985, vol. 31, no. 2, pp. 430–436.
Politov, D.V., Pirko, Y.V., Pirko, N.N., Mudrik, E.A., and Korshikov, I.I., Analysis of mating system in two Pinus cembra L. populations of the Ukrainian Carpathians, Ann. Forest Res., 2008, vol. 51, no. 1, pp. 11–18.
Restoux, G., Silva, D.E., Sagnard, F., Torre, F., Klein, E., and Fady, B., Life at the margin: the mating system of Mediterranean conifers, Web Ecol., 2008, vol. 8, pp. 94–102.
Korshikov, I.I., Mudrik, E.A., and Terlyga, N.C., Analysis of genetic variability of seed embryos in trees characterized by different seed productivity in Crimean population of Pinus pallasiana D. Don, Tsitol. Genet., 2005, vol. 39, no. 2, pp. 27–33.
Korshikov, I.I. and Mudrik, E.A., Comparative analysis of genetic heterogeneity of seed progenies in the isolated population of Pinus sylvestris var. cretacea Kalenicz ex Kom. in Donbass, Tsitol. Genet., 2006, vol. 40, no. 3, pp. 17–23.
Korshikov, I.I. and Mudrik, E.A., Elevation-dependent genetic variation of plants and seed embryos in the Crimea mountain population of Pinus pallasiana D. Don, Russ. J. Ecol., 2006, vol. 37, no. 2, pp. 79–83.
Privalikhin, S.N. and Makogon, I.V., Analysis of the mating system in two populations of Norway spruce (Picea abies (L.) Karst.) from Ukrainian Polissya, in The Science of Forest XXI Century: Proc. Int. Sci.-Pract. Conf., Gomel, 2010, pp. 268–269.
Altukhov, Yu.P., Genetic Processes in Populations, Moscow: Akademkniga, 2003.
Conservation and Management of Forest Genetic Resources in Europe, Geburek, Th. and Turok, J., Eds., Zvolen: Arbora Publ., 2005.
Korshikov, I.I., Kalafat, L.A., Lisnichuk, A.N., Velikorid’ko, T.I., and Mudrik, E.A., Allozyme variation of seed embryos and mating system in relict populations of Scots pine (Pinus sylvestris L.) from the Kremenets hill ridge and Maloe Poles’e, Russ. J. Genet., 2011, vol. 47, no. 7, pp. 829–835.
Hosius, B., Bergmann, F., Konnert, M., and Henkel, W., A concept for seed orchards based on isoenzyme gene markers, Forest Ecol. Manage, 2000, vol. 131, nos. 1–3, pp. 143–152.
Bush, I.R. and Smouse, P.A., Evidence for the adaptive significance of allozymes in forest trees, New Forests, 1992, vol. 6, no. 1, pp. 179–196.
Korshikov, I.I., Podgornyi, D.Yu., Kalafat, L.A., and Velikorid’ko, T.I., Population-and-genetic variation of the Pinus kochiana Klotzsch ex Koch in the Mountain Crimea, Rep. Natl. Acad. Sci. Ukraine, 2010, no. 5, pp. 161–167.
Korshikov, I.I., Podgorny, D.Yu., and Lisnichuk, A.N., Population and genetic differences between Koch pine (Pinus kochiana Klotzsch ex Koch) of mountain Crimea and Scotch pine (Pinus sylvestris L.) of Kremenets hill, Bul. Nikit. Botan. Sada, 2012, no. 104, pp. 22–26.
Korshikov, I.I., Velikorid’ko, T.I., Koba, V.P., Podgorny, D.Yu., Kalafat, L.A., and Gorlova, E.V., Genetic control of isozymes pine Koch in the mountain Crimea, Trudy Nikit. Bot. Sada, 2008, vol. 130, pp. 112–119.
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Original Ukrainian Text © I.I. Korshykov, L.A. Kalafat, O.M. Vynogradova, D.Y. Podgornyi, 2016, published in Tsitologiya i Genetika, 2016, Vol. 50, No. 2, pp. 36–43.
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Korshykov, I.I., Kalafat, L.A., Vynogradova, O.M. et al. Genetic variability of maternal plants and embryos of their seeds in the populations of Pinus kochiana Klotzsch ex Koch in Crimea. Cytol. Genet. 50, 110–115 (2016). https://doi.org/10.3103/S0095452716020079
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DOI: https://doi.org/10.3103/S0095452716020079