Abstract
The study has shown that both functional knockout of Sod1 and Sod2 genes encoding superoxide dismutase and their overexpression in neurons and glial tissue increases the sensitivity of Drosophila melanogaster to oxidative stress (OS) conditions. The lowest survival rate was only 20.5% in insects with Sod2 knockout in neurons. The comparative analysis of survival curves showed that adults with altered tissue-specific expression of the studied genes had reduced average and maximum life span. Under OS conditions induced by 5% hydrogen peroxide, the life spans of wild type Oregon R and transgenic insects were significantly reduced. Neurodegenerative phenotype is observed in 3-day-old imago with functional inactivation of Sod genes in glial tissue. During the aging of insects and the action of prooxidants, the increase of neurodegenerative phenotype is detected.
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Original Ukrainian Text © M.V. Vitushynska, N.P. Matiytsiv, Y.I. Chernyk, 2015, published in Tsitologiya i Genetika, 2015, Vol. 49, No. 2, pp. 21–28.
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Vitushynska, M.V., Matiytsiv, N.P. & Chernyk, Y.I. Influence of tissue-specific superoxide dismutase gene expression in brain cells on Drosophila melanogaster sensitivity to oxidative stress and viability. Cytol. Genet. 49, 95–101 (2015). https://doi.org/10.3103/S0095452715020127
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DOI: https://doi.org/10.3103/S0095452715020127