Abstract
Plants regenerated from hairy root culture and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with varying efficiency. The frequency of direct shoot formation from root cultures was up to 60% in Digitalis and 3.7% in Vinca. The addition of 1 mg/L BA, 0.1 mg/L NAA, and 5% sucrose to B5 medium increases regenerative capacity of periwinkle up to 19.1%. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology, and short internodes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kuzovkina, I. and Schneider, B., Genetically trans-formed root cultures-generation, properties and application in plant sciences, in Progress in Botany, Esser, K. et al., Eds., Berlin: Springer-Verlag, 2006, vol. 67, pp. 275–314.
Kuzovkina, I.N. and Vdovitchenko, M.Yu., Genetically transformed roots as a model system for studying physiological and biochemical processes in intact roots, Russ. J. Plant Physiol., 2011, vol. 58, no. 5, pp. 941–948.
Casanova, E., Trillas, M.I., Moysset, L., and Vainstein, A., Influence of rol genes in floriculture, Biotech. Adv., 2005, vol. 23, no. 1, pp. 3–39.
Christey, M.C. and Braun, R.H., Production of hairy root cultures and transgenic plants by Agrogbacterium rhizogenes-mediated transformation, Meth. Mol. Biol., 2005, vol. 286, pp. 47–60.
Christey, M.C., Use of Ri-mediated transformation for production of transgenic plants, In Vitro Cell Dev. Biol. Plant., 2001, vol. 37, pp. 687–700.
Lioshina, L.G., Bulko, O.V., and Galkin, A.P., Obtaining and characterization of callus and suspension cultures of periwinkle Vinca minor, in Factors of Experimental Evolution of Organisms, Kyiv: Logos, 2009, vol. 7, pp. 161–166.
Lioshina, L.G., Features of cultivation and growth characteristics of callus suspension culture of Digitalis prupurea L. cells, in Plant Physiology: Problems and Prospects, Kyiv: Logos, 2009, vol. 2, pp. 607–612.
Lioshina, L.G. and Bulko, O.V., Agrobacterium rhizogenes-mediated transformation and regeneration of two plant species from the family Apycynaceae Fiziol. Biokhim. Kul’tur. Roslin, 2011, vol. 43, no. 6, pp. 533–539.
Murashige, I. and Scoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, vol. 15, no. 3, pp. 473–497.
Gamborg, O.L. and Eveleigh, D.E., Culture methods and detection of glucanases in cultures of wheat and barley, Can. J. Biochem., 1968, vol. 46, no. 5, pp. 417–421.
Markovskaya, E.F., Sysoeva, M.I., Trofimova, S.A., and Kurets, V.K., Matematicheskie metody opredeleniya nekotorykh biometricheskikh pokazatelei u rastenii (Mathematical Methods for Determination of Certain Biometric Indices in Plants), Petrozavodsk, 1988.
Lakin, G.F., Biometriya (Biometry), Moscow: Vysshaya Shkola, 1990.
Tanaka, N., Takao, M., and Matsumoto, T., Agrobacterium rhizogenes-mediated transformation and regeneration of Vinca moinor L., Plant Tissue Cult. Lett., 1994, vol. 11, no. 3, pp. 191–198.
Feng, B., Zhao, C.H., and Wang, G.L., Transformation of Cucumis sativus by A. rhizogenes and regeneration of transformed hairy root, J. Liaoning Norm. Univ., 2000, vol. 23, pp. 171–174.
Pradel, H., Dumke-Lebmann, U., Dietrich, B., and Luckner, M., Hairy root cultures of Digitalis lanata. Secondary metabolism and plant regeneration, J. Plant. Physiol., 1997, vol. 151, pp. 209–215.
Koga, M., Hirashima, K., and Nakahara, T., The transformation system in foxglove (Digitalis purpurea L.) using Agrobacterium rhizogenes and traits of the regenerants, Plant Biotechnol., 2000, vol. 17, no. 2, pp. 99–104.
Dinkar, V.P., White, F.F., Nester, E.W., and Gordon, M.P., rolA Locus of the Ri plasmid directs developmental abnormalities in transgenic tobacco plants, Genes Dev., 1988, vol. 2, pp. 688–697.
Schmulling, T., Schell, J., and Spena, A., Single genes from Agrobacterium rhizogenes influence plant development, EMBO J., 1988, vol. 7, pp. 2621–2629.
Koltunow, A.M., Johnson, S.D., and Lynch, M., Expression of rolB in apomictic Hieracium piloselloides Vill. causes ectopic meristems in planta and changes in ovule formation, where apomixes initiates at higher frequency, Planta, 2001, vol. 214, pp. 196–205.
Boase, M.R., Winefield, C.S., and Lill, T.A., Transgenic regal pelargoniums that express the rolC gene from Agrobacterium rhizogenes exhibit a dwarf floral and vegetative phenotype, In Vitro Cell. Dev. Biol., 2004, vol. 40, pp. 46–50.
Spena, A., Schmiilling, T., Konez, C., and Schell, J.S., Independent and synergistic activity of rol A, B and C loci in stimulation of abnormal growth in plants, EMBO J., 1987, vol. 13, no. 13, pp. 3891–3899.
Binns, A.N. and Costantino, P., The Agrobacterium oncogenes, in The Rhizobiaceae, Dordrecht: Kluwer Press, 1998, pp. 251–266.
Meyer, A.D., Tempé, J., and Costantino, Hairy root: a molecular overview. Functional analysis of Agrobacterium rhizogenes T-DNA genes, in Plant-Microbe Interactions, St. Paul: APS Press, 2000, pp. 93–139.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © L.G. Lioshina, O.V. Bulko, 2014, published in Tsitologiya i Genetika, 2014, Vol. 48, No. 5, pp. 36–42.
About this article
Cite this article
Lioshina, L.G., Bulko, O.V. Plant regeneration from hairy roots and calluses of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L.. Cytol. Genet. 48, 302–307 (2014). https://doi.org/10.3103/S009545271405003X
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S009545271405003X