Skip to main content

Differentiation of haploid and dihaploid rape plants at the cytological and morphological levels

Abstract

Some cytological and morphological features of haploid and dihaploid winter rapó plants obtained via the anther cultivation approach have been studied. It was shown that in haploid plants the number of chloroplasts in stomatal guard cells, the size of the stomatal guard cells themselves were much smaller, and the number of stomata per square unit was greater than in doubled haploids and diploids. Haploids were also characterized by smaller sizes of petals and anthers and, in general, a smaller flower as compared to dihapliods and diploids.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Hu, H. and Zeng, J.Z., Development of New Varieties via Anther Culture, in Handbook of Plant Cell Culture, New York: Macmillan, 1984, vol. 3, pp. 65–90.

    Google Scholar 

  2. 2.

    Morrison, R.A. and Evans, D.A., Haploid Plants from Tissue Culture: New Plant Varieties in a Shortened Time, Biotechnology, 1988, vol. 6, pp. 684–690.

    Article  Google Scholar 

  3. 3.

    Nietzsche, B. and Wenzel, G., Gaploidy v selektsii rastenii (Haploids in Plant Breeding), Moscow: Kolos, 1980.

    Google Scholar 

  4. 4.

    Lyakh, V.A. and Soroka, A.I., Botanicheskie i tsitogeneticheskie osobennosti vidov roda Linum L. i biotekhnologicheskie puti raboty s nimi (Botanical and Cytogenetic Characteristics of the Genus Linum L. and Biotechnological Ways to Work with Them), Zaporozh’e, 2008.

    Google Scholar 

  5. 5.

    Ferrie, A., Microspore Culture of Brassica Species, in Doubled Haploid Production in Crop Plants: A Manual, Maluszynski, M., et al., Eds., Dordrecht: Kluwer, 2003, pp. 205–215.

    Google Scholar 

  6. 6.

    Shamina, Z.B., Androgenesis and Obtaining Haploids in Anther and Microspore Cultures, in Kul’tura kletok rastenii (Plant Cell Culture), Butenko, R.G., Ed., Moscow: Nauka, 1981, pp. 124–135.

    Google Scholar 

  7. 7.

    Vyvadilova, M., Tomaskova, D., Bechyne, M., and Kucera, V., The Use of Doubled Haploids to Stabilize Yellow-Seedness in Oilseed Rape (Brassica napus), Czech J. Genet. Plant Breed., 1999, vol. 35, no. 1, pp. 7–9.

    Google Scholar 

  8. 8.

    Shipeng, L., Combination of Reversible Male Sterility and Doubled Haploid Production by Targeted Inactivation of Cytoplasmic Glutamine Synthetase in Developing Anthers and Pollen, Plant Biotech. J., 2007, vol. 5, no. 4, pp. 483–494.

    Article  Google Scholar 

  9. 9.

    Keller, W.A. and Armstrong, K.C., Embryogenesis and Plant Regeneration in Brassica napus Anther Cultures, Can. J. Bot., 1977, vol. 55, pp. 1383–1388.

    Article  Google Scholar 

  10. 10.

    Custers, J.B.M., Cordewener, J.H.G., Noellen, Y., Dons, H.J.M., Van Lookeren Champagne M.M. Temperature Controls Both Gametophytic and Sporophytic Development in Microspore Cultures of Brassica napus, Plant Cell Rep., 1994, vol. 13, pp. 267–271.

    Article  CAS  Google Scholar 

  11. 11.

    Friedt, W. and Zarhloul, M.K., in Haploids in Crop Improvement II, Palmer, C.E. and Keller, W.A., Kasha, K.J., Eds., Berlin: Springer, 2005, vol. 56, pp. 191–213.

  12. 12.

    Zhou, W.J., Tang, G.X., and Hagberg, P., Efficient Production of Doubled Haploid Plants by Immediate Colchicine Treatment of Isolated Microspores in Winter Brassica Napus, Plant Growth Regulation, 2002, vol. 37, no. 2, pp. 185–192.

    Article  CAS  Google Scholar 

  13. 13.

    Pausheva, Z.P., Praktikum po tsitologii rastenii (A Practical Course in Plant Cytology), Moscow: Kolos, 1980.

    Google Scholar 

  14. 14.

    Dospekhov, B.A., Metodika polevogo opyta (Field Experiment Method), Moscow: Kolos, 1979.

    Google Scholar 

  15. 15.

    Khokhlov, S.S., Tyrnov, V.S., Grishina, E.V., et al., Gaploidiya i selektsiya (Haploidy and Breeding), Moscow: Nauka, 1976.

    Google Scholar 

  16. 16.

    Hamaoka, Y., Fujita, Y., and Iwai, S., Number of Chloroplasts in Haploids and Diploids Produced via Anther Culture in Brassica campestris, Plant Tissue Culture Lett., 1991, vol. 8, no. 2, pp. 67–72.

    Article  Google Scholar 

  17. 17.

    Qin, X. and Rotino, G.L., Chloroplast Number in Guard Cells as Ploidy Indicator of in vitro-Grown Androgenic Pepper Plantlets, Plant Cell Tissue Organ Culture, 1995, vol. 41, no. 2, pp. 145–149.

    Article  Google Scholar 

  18. 18.

    Davydova, N.N., Improvement of the Anther Culture Method for Use in Cabbage (Brassica oleracea L.) Breeding, Extended Abstract of Cand. Sci. (Agric.) Dissertation, Moscow, 2008.

    Google Scholar 

  19. 19.

    Kotlyarova, O.V., Improving the Technology for Obtaining Regenerants to Create Double Haploids of Carrot (Daucus carota L.), Extended Abstract of Cand. Sci. (Agric.) Dissertation, Moscow, 2010.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. I. Soroka.

Additional information

Original Russian Text © A.I. Soroka, 2013, published in Tsitologiya i Genetika, 2013, Vol. 47, No. 2, pp. 34–39.

About this article

Cite this article

Soroka, A.I. Differentiation of haploid and dihaploid rape plants at the cytological and morphological levels. Cytol. Genet. 47, 88–92 (2013). https://doi.org/10.3103/S0095452713020102

Download citation

Keywords

  • Guard Cell
  • Anther Cultivation
  • Microspore Culture
  • Haploid Plant
  • Diploid Plant