Abstract
An analysis of numerical anomalies of chromosomes 13, 18, 21, X, and Y was carried out using the FISH method before in vitro fertilization in the sperm nuclei of patients with astheno-, oligo, and teratozoospermia. The percentage of aneuploid spermatozoa was significantly higher (p < 0.05) in patients with oligozoospermia compared to patients with astheno- and teratozoospermia. A significant difference in the percentage of spermatozoa that carried a cytoplasmic droplet was revealed in patients with a content of aneuploid sperm of more than 1.0% and less than 0.2%.
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References
Ailamazyan, E.K., Materialy XXV nauchnoi sessii XXV akusherstva i ginekologii (Proc. XXV Scientific Session of the Research Institute of Obstetrics and Gynecology), Moscow, 1996.
Vorsanova, S.G., Beresheva, A.K., Kazantseva, L.Z., Demidova, I.A., and Sharonin, V.O., Molecular-Cytogenetic Diagnosis of Chromosomal Abnormalities in Couples with Reproductive Function Disorders, Probl. Reprod., 1998, no. 4, pp. 41–46.
Kulakov, V.I. and Leonov, B.V., Ekstrakorporal’noe oplodotvorenie i ego novye napravleniya v lechenii zhenskogo i muzhskogo besplodiya (teoreticheskie i prakticheskie podkhody): Rukovodstvo dlya vrachei (In vitro Fertilization and New Directions in the Treatment of Male and Female Infertility (Theoretical and Practical Approaches): A Guide for Doctors), Moscow: Med. Inform. Agentstvo, 2004.
Dohle, G.R., Diemer, T., Giwercm, A., Jungwirth, A., Kopa, Z., and Krausz, C., Muzhskoe besplodie (Male Infertility), Akopyan, A.S., Ed., Evrop. Assots. Urologov, 2010.
Sarah, E.D., Sean, P.F., and Colin, D.M., Detection of Chromosomes and Estimation of Aneuploidy in Human Spermatozoa Using in-situ Hybridization, Mol. Hum. Rep., 1997, vol. 3, no. 7, pp. 585–598.
Staessen, C., Hortournaye, H., Michiels, A., Devroey, P., et al., PGD in 47, XXY Klienfelter’s Syndrome Patients, Hum. Rep., 2003, vol. 9, no. 4, pp. 319–330.
Chantot-Bastaraud, S., Ravel, C., Berthaut, I., McElreavey, K., et al., Sperm-FISH Analysis in a Pericentric Chromosome 1 Inversion, 46, XY, Inv(1) (P22q42), Associated with Infertility, Mol. Hum. Rep., 2007, vol. 13, no. 1, pp. 55–59.
Cassuto, G.N., Plouchart, J.M., Balet, R., et al., Interest of a New Morphological Classification of Human Spermatozoa for ICSI Allowing to Obtain a Better Blastocyst Score, Hum. Rep., 2007, vol. 22,suppl. 1, p. i113.
Acar, H., Kilinc, M., Guven, S., Yurdakul, T., and Celik, R., Comparison of Semen Profile and Frequency of Chromosome Aneuploidies in Sperm Nuclei of Patients with Varicocele before and after Varicocelectomy, Andrologia, 2009, vol. 41, no. 3, pp. 157–162.
Leont’eva, O.A. and Vorob’eva, O.A., Comparative Analysis of Human Sperm Morphology: Native Ejaculate, Progressively Motile Fraction, Probl. Reprod., 1999, no. 3, pp. 29–36.
Glants, S., Mediko-biologicheskaya statistika (Biomedical Statistics), Moscow: Praktika, 1999.
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Original Russian Text © A.M. Fes’kov, I.S. Zhylkova, I.M. Bezpechnaya, O.V. Somova, V.A. Fes’kov, 2013, published in Tsitologiya i Genetika, 2013, Vol. 47, No. 2, pp. 66–69.
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Fes’kov, A.M., Zhylkova, I.S., Bezpechnaya, I.M. et al. Correlation of the sperm morphology with the presence of aneuploidies in its nuclei in patients with astheno-, oligo-, and teratozoospermia. Cytol. Genet. 47, 115–117 (2013). https://doi.org/10.3103/S0095452713020059
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DOI: https://doi.org/10.3103/S0095452713020059