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Obtaining of hairy-root, callus and suspenison cell cultures of carrot (Daucus carota L.) able to accumulate human interferon alpha-2b

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Abstract

Here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of Nantskaya and Perfektzya variety with the highest level of recombinant human interferon-2b accumulation exhibiting the highest level of plant protein extract antiviral activity (up to 12.8 × 103 IU/mg TSP). The antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from the parent organisms. However, the antiviral activity level of suspension culture extracts (up to 4.42 × 103 IU/mg TSP) and Ri-root ones (up to 4.42 × 103 IU/mg TSP) appeared to be comparable to analogical data of antiviral activity of transgenic carrot leaf extracts, this way the described cultures could be possibly used for comparatively speedy obtaining of recombinant therapeutic protein for curing and preventing of virus diseases.

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Author information

Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, vul. Acad. Zabolotnogo 148, Kiev, 03680, Ukraine

    Yu. S. Luchakivskaya, E. M. Kishchenko & N. V. Kuchuk

  2. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Academician Zabolotny, Kiev, 03143, Ukraine

    Z. M. Olevinskaya & N. Ya. Spivak

Authors
  1. Yu. S. Luchakivskaya
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  2. Z. M. Olevinskaya
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  3. E. M. Kishchenko
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  4. N. Ya. Spivak
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  5. N. V. Kuchuk
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Corresponding author

Correspondence to Yu. S. Luchakivskaya.

Additional information

Original Russian Text © Yu.S. Luchakivskaya, Z.M. Olevinskaya, E.M. Kishchenko, N.Ya. Spivak, N.V. Kuchuk, 2012, published in Tsitologiya i Genetika, 2012, Vol. 46, No. 1, pp. 18–26.

The article was translated by the authors.

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Luchakivskaya, Y.S., Olevinskaya, Z.M., Kishchenko, E.M. et al. Obtaining of hairy-root, callus and suspenison cell cultures of carrot (Daucus carota L.) able to accumulate human interferon alpha-2b. Cytol. Genet. 46, 15–20 (2012). https://doi.org/10.3103/S0095452712010057

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  • DOI: https://doi.org/10.3103/S0095452712010057

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