Abstract
The optical density indexes of nucleoli and cytoplasm of male generative cells during microsporogenesis have been determined for wheat, rye, and F1 of wheat-rye hybrids using RNA staining. The positive correlation between the RNA content in nucleoli and cytoplasm of the cells of all plants has been found. The dynamics of correlation links between the nucleolus volume and RNA content in the nucleolus/cytoplasm of the wheat and wheat-rye hybrids in microsporogenesis and early gametogenesis have been shown. The essential species and sort differences based on quantitative cytochemical and karyometrical characteristics in parental forms and the dependence of their appearance in interspecies F1 hybrids on wheat’s maternal form have been identified.
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References
Tyrnov, V.S., Plant Embryogenetics, in Embriologiya tsvetkovykh rastenii. T. 3. Reproduktivnye sistemy (Embryology of Flowering Plants. Vol. 3: Reproductive Systems), St. Petersburg: Mir i sem’ya, 1994, pp. 389–392.
Olson, M.O., Hingorani, K., and Szebeni, A., Conventional and Nonconventional Roles of the Nucleolus, Int. Rev. Cytol., 2002, vol. 219, pp. 199–266.
Zharskaya, O.O. and Zatsepina, O.V., Dynamics and Mechanisms of Nucleolar Reorganization during Mitosis, Tsitologiya, 2007, vol. 49, no. 5, pp. 355–369.
Caspersson, T.O., Cell Growth and Cell Function, New York: Norton, 1950.
Blankovskaya, T.F. and Trochinskaya, T.G., Cytological Markers of rRNA Gene Expression during Microsporogenesis in Rye, Wheat and Wheat-Rye Hybrids, Tsitol. Genet., 2005, vol. 39, no. 2, pp. 22–26.
Chelidze, P.V., Ul’trastruktura i funktsii yadryshka interfaznoi kletki (Ultrastructure and Functions of the Nucleolus of an Interphase Cell), Tbilisi: Metsniereba, 1985.
Sobol’, M.A., Role of the Nucleolus in Plant Cell Response to Environmental Physical Factors, Tsitol. Genet., 2001, vol. 35, no. 3, pp. 72–84.
Pausheva, Z.P., Praktikum po tsitologii rastenii (A Practical Course in Plant Cytology), Moscow: Agropromizdat, 1988.
Palamarchuk, I.A. and Veselova, T.D., Uchebnoe posobie po botanichkeskoi gistokhimii (A Manual on Botanical Histochemistry), Moscow: Izd. Mosk. Gos. Univ., 1965.
Dospekhov, B.A., Metodika polevogo opyta (Field Experiment Method), Moscow: Kolos, 1985.
Arkhipchuk, V.V., The Use of Nucleolar Characteristics in Biotesting, Tsitol. Genet., 1995, vol. 29, no. 3, pp. 6–12.
Trochinskaya, T., The Constance of Nucleolus Volume Magnitude in the Male Generative Structures Cells of Wheat and Wheat-Rye Hybrids, Biodiversity. Ecology. Adaptation. Evolution: III Intern. Young Science Conf., Odessa, 2007, p. 228.
Vakar, B.A., Results of Comparative Study of Meiosis and Hybrids of the Genus Triticum L. with Representative of Genera Secale L., Aegilops L., and Agropiron Gaerth, Tr. Belorus. S.-Kh. Inst., 1948, vol. 3, no. 1, pp. 16–43.
Shkurenko, S.V., On the Problem of Sterility of Wheat-Rye Hybrids, Tr. Inst. Botan. AN KazSSR, 1968, pp. 57–70.
Shchapova, A.I. and Kravtsova, L.A., Tsitogenetika pshenichno-rzhanykh gibridov (Cytogenetics of Wheat-Rye Hybrids), Novosibirsk: Nauka, 1990.
Silkova, O.G., Shchapova, A.I., and Kravtsova, L.A., Mechanisms of Meiotic Restitution and Their Genetic Regulation in Wheat-Rye Polyhaploids, Genetika, 2003, vol. 29, no. 11, pp. 1505–1515.
Petrova, K.A., Characteristics of Sterility of Wheat-Elimus Hybrids of the First Generation and the Possibility of Its Overcoming, in Otdalennaya gibridizatsiya i poliploidiya (Remote Hybridization and Polyploidy), Moscow: Nauka, 1970, pp. 58–77.
Bazilinskaya, N.V., Histochemical Characteristics of Generative Organs of the Wheat-Agropyron Hybrid, Byul. Glav. Bot. Sada, 1967, no. 65, pp. 32–35.
Blankovskaya, T.F. and Vorob’ev, A.I., Cytochemical Study of Male Gametophyte in Wheat Interspecific Hybrids, Tsitol. Genet., 1971, vol. 5, no. 3, pp. 202–206.
Reznikova, S.A., Tsitologiya i fiziologiya razvivayushchegosya pyl’nika (Cytology and Physiology of Developing Anther), Moscow: Nauka, 1984.
Efimov, V.K., Galaktionov, Yu.K., and Galaktionova, N.S., Relationship between Correlation and Variation Coefficient Values and Absolute Character Values, Zh. Obshch. Biol., 1977, vol. 38, no. 1, pp. 24–26.
Skuridin, G.M. and Koval’, S.F., Genotype Identification on the Basis of Phenotype using Correlation of Characters, Inf. Vestn. VOGiS, no. 19, pp. 12–18.
Zhuchenko, A.A., Ekologicheskaya genetika kul’turnykh rastenii i problemy agrosfery (Ecological Genetics of Cultivated Plants and Problems of the Agrosphere), Moscow: Agrorus, 2004.
Lykova, N.A., Variability of Genetic-Statistical Characters of Triticum aestivum and Hordeum vulgare in Ontogeny, Nauch. Zhurn. KubGAU, 2006, no. 8, pp. 11–18.
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Original Russian Text © T.G. Trochinskaya, T.F. Blankovskaya, V.N. Totskii, 2010, published in Tsitologiya i Genetika, 2010, Vol. 44, No. 4, pp. 48–54.
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Trochinskaya, T.G., Blankovskaya, T.F. & Totskii, V.N. Quantitative characters of male generative structure cells of wheat, rye, and wheat-rye hybrids during microsporogenesis. Cytol. Genet. 44, 233–238 (2010). https://doi.org/10.3103/S0095452710040080
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DOI: https://doi.org/10.3103/S0095452710040080