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Enzyme-Linked Immunosorbent Assay of Autoantibodies against the Human β1-Adrenergic Receptor Using Recombinant Antigens

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Moscow University Chemistry Bulletin Aims and scope

Abstract

E. coli strains are created as producers of recombinant β1-adrenoreceptor epitopes as part of chimeric proteins. The corresponding epitope sequences are located in the C-terminal region of the human heart fatty acid binding protein (hH-FABP) and separated from it by the linker sequence (Gly4Ser)3. A solid-phase enzyme-linked immunosorbent assay (ELISA) to detect autoantibodies against the β1-adrenergic receptor in human blood serum based on a recombinant epitope is developed. Blood sera of patients (N = 76) with various diagnoses of cardiopathologies and other diseases are analyzed. A significantly high level of autoantibodies to the β1-adrenergic receptor is detected in some patients with a confirmed diagnosis of cardiovascular diseases, in most cases those with a diagnosis of acute myocardial infarction.

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Funding

This study was performed as part of state task of Moscow State University, State grant no. 121041500039-8 (Molecular Design, Structural, and Functional Analysis and Regulation of Enzyme Systems, Cell Structures, and Bionanomaterials: Fundamental Principles and Applications in Technology, Medicine, and Environmental Protection).

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    V. G. Grigorenko, I. P. Andreeva, E. A. Melnichuk & P. A. Levashov

Authors
  1. V. G. Grigorenko
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  2. I. P. Andreeva
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  3. E. A. Melnichuk
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  4. P. A. Levashov
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Corresponding author

Correspondence to V. G. Grigorenko.

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The authors declare that they have no conflicts of interest.

Additional information

Translated by A. Tulyabaew

Abbreviations: ELISA, enzyme-linked immunosorbent assay; hH-FABP, human heart fatty acid binding protein; DCM, dilated cardiomyopathy.

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Grigorenko, V.G., Andreeva, I.P., Melnichuk, E.A. et al. Enzyme-Linked Immunosorbent Assay of Autoantibodies against the Human β1-Adrenergic Receptor Using Recombinant Antigens. Moscow Univ. Chem. Bull. 78, 95–101 (2023). https://doi.org/10.3103/S0027131423020025

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  • DOI: https://doi.org/10.3103/S0027131423020025

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