Abstract
A turbidimetric method for determining the activity of bacteriolytic enzymes against living Lactobacillus plantarum cells as a substrate is developed. A technique for measuring the sorption of an enzyme on living cells in an experiment for activity determination is described, with chicken egg lysozyme being used as a standard model enzyme. The correctness of the calculations of the kinetic parameters is proved by counting the colony-forming units (CFUs). The physical and chemical parameters of the sorption of the enzyme on the cells are calculated and the correctness of the obtained equilibrium desorption constants is confirmed.
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Original Russian Text © D.A. Matolygina, H.E. Osipova, S.A. Smirnov, N.G. Belogurova, N.L. Eremeev, V.I. Tishkov, A.V. Levashov, P.A. Levashov, 2015, published in Vestnik Moskovskogo Universiteta. Khimiya, 2015, No. 6, pp. 365–371.
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Matolygina, D.A., Osipova, H.E., Smirnov, S.A. et al. Determination of the activity of bacteriolytic enzymes and measurement of their sorption in the system of living cells of Lactobacillus plantarum . Moscow Univ. Chem. Bull. 70, 292–297 (2015). https://doi.org/10.3103/S002713141506005X
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DOI: https://doi.org/10.3103/S002713141506005X