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Moscow University Chemistry Bulletin

, Volume 69, Issue 3, pp 112–116 | Cite as

An investigation of the physicochemical properties of both glutathione peroxidase I and its complexes with polyelectrolytes as promising agents for the treatment of diseases of the central nervous system

  • I. S. Panina
  • L. Y. Filatova
  • A. V. Kabanov
  • N. L. Klyachko
Article
  • 37 Downloads

Abstract

A study of the kinetics of the thermal inactivation of glutathione peroxidase I was carried out. Glutathione peroxidase is an enzyme that plays a key role in the antioxidant protection system of the body. It was shown that oligomeric glutathione peroxidase I is inactivated by a monomolecular mechanism (37°C, pH 7.0, the enzyme concentrations 0.3 and 1 mg/mL). An effective method for stabilizing the enzyme by polyelectrolytes of different natures was proposed.

Keywords

reactive oxygen species glutathione peroxidase I polyelectrolyte kinetics of inactivation stabilization 

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References

  1. 1.
    Sies, H. and Helmut, M., Exp. Phys., 1997, vol. 82, p. 291.Google Scholar
  2. 2.
    Skulachev, V.P., Soros. Obraz. Zh., 1996, vol. 3, p. 4.Google Scholar
  3. 3.
    Mills, G., Arch. Biochem. Biophys., 1960, vol. 86, p. 1.CrossRefGoogle Scholar
  4. 4.
    Nagababu, E., Chrest, F., and Rifkind, J., Biochim. Biophys. Acta, 2003, vol. 25, p. 1.Google Scholar
  5. 5.
    Rotruck, J. and Pope, A., Science, 1973, vol. 179, p. 588.CrossRefGoogle Scholar
  6. 6.
    Flohe, R., Free Rad. Biol. Med., 1999, vol. 27, p. 951.CrossRefGoogle Scholar
  7. 7.
    Arthur, J., Cell. Mol. Life Sci., 2000, vol. 57, p. 1825.CrossRefGoogle Scholar
  8. 8.
    Okovityi, S.V., FARMind.: Prakt., 2003, vol. 5, p. 85.Google Scholar
  9. 9.
    Muller, F., Lustgarten, M., and Jang, Y., Free Rad. Biol. Med., 2007, vol. 43, p. 477.CrossRefGoogle Scholar
  10. 10.
    Koller, L., South, P., Exon, J., and Whitbeck, G., Can. J. Comp. Med., 1984, vol. 48, p. 431.Google Scholar
  11. 11.
    Yacheika dlya elektroforeza Mini-PROTEANTM Tetra. Instruktsiya po ekspluatatsii (A Camber for Electrophoresis Mini-PROTEANTM Tetra: Operating Instruction), p. 14.Google Scholar
  12. 12.
    Miwa, S., Nakashima, K., Ariyoshi, E., et al., Br. J. Haematol., 1975, vol. 29, p. 157.CrossRefGoogle Scholar
  13. 13.
    Toppo, S., Flohe, L., and Ursini, F., Biochem. Biophys. Acta, 2009. 1790, p. 1486.CrossRefGoogle Scholar
  14. 14.
    Asahi, M., Fujii, J., and Takao, T., J. Biol. Chem., 1997, vol. 272, p. 19152.CrossRefGoogle Scholar

Copyright information

© Allerton Press, Inc. 2014

Authors and Affiliations

  • I. S. Panina
    • 1
  • L. Y. Filatova
    • 1
  • A. V. Kabanov
    • 1
    • 2
  • N. L. Klyachko
    • 1
  1. 1.Department of ChemistryMoscow State UniversityMoscowRussia
  2. 2.The Center for Nanotechnology in Drug Delivery, Carolina Institute for NanomedicineUniversity of North CarolinaChapel HillUSA

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