Catalytic characteristics of enzyme-polyelectrolyte complexes based on hexahistidine-containing organophosphorus hydrolase
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A number of covalent and non-covalent polyelectrolyte complexes based on enzyme such as hexahistidine-containing organophosphorus hydrolase was developed in this work. Polyanions and polycations were used in the form of block-copolymers with poly(ethylene glycol). To obtain covalent complexes of the enzyme, different coupling agents (glutaric aldehyde, N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt) were tried. The best catalytic efficiency of the action of the covalently bound complex ((2.8 ± 0.3) × 108 M−1 s−1) was obtained for samples with EDC. The modification of the protein surface was undertaken as an approach to its further biomedical application.
Keywordshexahistidine-containing organophosphorus hydrolase polyelectrolyte enzyme-polyelectrolyte complex
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