Phase visualization in the study of cellular structures by confocal microscopy
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It is experimentally shown that recording of the axial hologram, along with the confocal image, expands the possibilities of studying biological structures. Specifically, this approach makes it possible to visualize the phase of object wave without significant changes in the confocal microscope design and using standard methods for image processing.
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- 2.G. I. Shtein, Guidance on Confocal Microscopy (Izd-vo SPU, St. Petersburg, 2007) [in Russian].Google Scholar
- 3.M. E. Cox, “Microscopy,” in Handbook of Optical Holography, Ed. by H. J. Caulfield (Academic Press, N.Y., 1979).Google Scholar
- 7.M. I. Mityushov, N. A. Emel’yanov, A. A. Mokrushin, I. A. Voiner, and T. R. Bagaeva, Living Brain Slice as an Object of Neurophysiological and Neurochemical Study (Nauka, Leningrad, 1986) [in Russian].Google Scholar
- 8.I. V. Mukhina, V. B. Kazantsev, L. G. Khaspekov, Yu. N. Zakharov, M. V. Vedunova, E. V. Mitroshina, S. A. Korotchenko, and E. A. Koryagina, “Multielectrode Matrices: New Possibilities in Studying the Plasticity of Neuronal Network,” Sovremennye Tekhnologii v Meditsine. No. 1, 8 (2009).Google Scholar
- 9.V. V. Lobyntseva and Yu. N. Zakharov, “Application of DigitalHolography for Studying Cellular Structures,” in Proceedings of the 13th International Conference of Students and Young Researchers “Youth and Science” (Izd-vo NIYaU MIFI, Moscow, 2010), Pt. 3, p. 128 [in Russian].Google Scholar
- 11.J. B. DeVelis and G. O. Reynolds, “Fresnel Holograms,” in Handbook of Optical Holography, Ed. by H. J. Caulfield (Academic Press, N.Y., 1979).Google Scholar