Physical Interactions of Biopolymers with Nanoparticles
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Using fluorescence resonance energy transfer, the physical interaction of nanoparticles with biopolymers is studied by the example of the interaction of quantum dots (energy donor) with two-chain oligonucleotides labeled with a fluorescent organic dye BDP TR (energy acceptor). The high-efficiency resonance energy transfer from quantum dots to an organic dye not only makes it possible to study the dependence of the transfer efficiency on the donor-acceptor molar ratio but also represents an efficient tool for studying the interactions of oligonucleotides of different structures with nanoscale objects.
Keywordsquantum dots oligonucleotides fluorescence resonance energy transfer FRET
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This study was supported by the Ministry of Higher Education and Science of the Russian Federation, project no. 14.Y26.31.0011.
- 4.S. B. Brichkin, Khim. Vys. Energ. 47, 411 (2013) [High Energy Chem. 47, 277 (2013)].Google Scholar