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Molecular Genetics, Microbiology and Virology

, Volume 32, Issue 2, pp 109–115 | Cite as

Prevalence and molecular characterization of class 1 integrons among clinical isolates of Pseudomonas aeruginosa in Northwest of Iran

  • Hamid Reza Goli
  • Mohammad Reza Nahaei
  • Mohammad Ahangarzadeh Rezaee
  • Alka Hasani
  • Hossein Samadi Kafil
  • Mohammad AghazadehEmail author
  • Vajihe Sheikhalizadeh
Experimental Works

Abstract

Introduction

Integrons are introduced as the most common mechanism for resistance gene dissemination in Pseudomonas aeruginosa.

Aims and tasks

The purpose of the present study was to determine the diversity of the gene cassettes carried with class 1 integrons in clinical isolates of Pseudomonas aeruginosa.

Methods and results

One hundred clinical isolates of Pseudomonas aeruginosa from four hospitals in Northwest of Iran were investigated for antimicrobial susceptibility, presence of class 1 integrons and associated resistance gene cassettes. Seventy-one percent of isolates were multidrug resistant (MDR) and colistin was the most effective antibiotic in this study. Sixty-six isolates (66%) were contained class 1 integrons which all of them carried gene cassettes. There was a strong correlation between integron presence and multidrug resistance phenotype (p < 0.05). Amplification of the internal variable regions of class 1 integrons revealed 10 different arrays ranging in size from 0.6 to 3.5 kb. DNA sequencing analysis of class 1 integrons revealed the presence of several gene cassettes associated with resistance to aminoglycosides (aac and aad), β-lactams (bla OXA), chloramphenicol (cmlA and catB), hypothetical gene cassette encoding a hypothetical protein and an open reading frame with unknown function (orfD). The most prevalent gene cassette found within class 1 integrons was aadB (68.18%), followed by aacA4 (48.48%). To the best of our knowledge, some of the gene cassettes arrays such as aac(3)-Ic-aacA5-cmlA5 and aacA5-aadA1-cmlA5 were first identified in this study. Hospital ward was demonstrated as significant risk factors for the acquisition of class 1 integrons harboring isolates in this study (P < 0.05).

Conclusions

Most of gene cassette arrays in this study are reported for the first time in Iran. These show the role of integrons in dissemination of resistance genes among clinical isolates of P. aeruginosa.

Keywords

Pseudomonas aeruginosa antimicrobial resistance class 1 integron gene cassette 

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Copyright information

© Allerton Press, Inc. 2017

Authors and Affiliations

  • Hamid Reza Goli
    • 1
    • 3
    • 4
  • Mohammad Reza Nahaei
    • 1
    • 3
  • Mohammad Ahangarzadeh Rezaee
    • 2
    • 3
  • Alka Hasani
    • 2
    • 3
  • Hossein Samadi Kafil
    • 1
    • 3
  • Mohammad Aghazadeh
    • 1
    • 3
    Email author
  • Vajihe Sheikhalizadeh
    • 3
  1. 1.Drug Applied Research Center, Faculty of MedicineTabriz University of Medical SciencesTabrizIran
  2. 2.Infectious Diseases and Tropical Medicine Research Center, Faculty of MedicineTabriz University of Medical SciencesTabrizIran
  3. 3.Department of Medical Microbiology and Virology, Faculty of MedicineMazandaran University of Medical SciencesSariIran
  4. 4.Student Research CommitteeTabriz University of Medical SciencesTabrizIran

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