Molecular Genetics, Microbiology and Virology

, Volume 30, Issue 4, pp 221–224 | Cite as

Prevalence of fibronectin-binding protein (FnbA and FnbB) genes among clinical isolates of methicillin resistant Staphylococcus aureus

  • Mohsen Mirzaee
  • Shahin Najar-PeerayehEmail author
  • Mehrdad Behmanesh
Experimental Works


One of the most important stages in the occurrence of infection caused by Staphylococcus aureus is able to adhere of the bacterium to the cells and the extracellular matrix. Among adhesins, two fibronectinbinding proteins, (FnbA and FnbB) have been proved significantly to contribute to tissue colonization in various pathological conditions and indwelling medical device related infections. The aims of this study were to detect fnbA and fnbB genes and relation to the fibronectin binding ability in clinical isolates of methicillin resistant Staphylococcus aureus (MRSA). Of the 62 MRSA clinical isolates collected from selected hospitals in Tehran, Iran, fibronectin binding ability was determined by microtiter tissue culture coated by fibronectin plates. All MRSA isolates were examined for determination the fnbA and fnbB genes by using PCR method. The prevalence of fnbA and fnbB in MRSA strains was 82.2 and 46.7% respectively. In 26 (42%) MRSA strains both fnbA and fnbB genes were positive. All fnbA + or fnbB + strains were found to be positive also to in vitro fibronectin binding activity, while the fnb-negative strains were found negative to the in vitro binding assay. The finding that, fibronectin-adhesins are present in the most of the MRSA clinical isolates encourages the development of strategies to specifically block the interaction of this bacterium with fibronectin by antagonist molecules.


fibronectin-binding proteins MRSA Bacterial adhesion 


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  1. 1.
    Ghasemian, A., Peerayeh, S.N., Bakhshi, B., and Mirzaee, M., Detection of accessory gene regulator groups genes and cassette chromosome mec types among Staphylococcus aureus isolated from intensive care unit patients, Asian Pac. J. Trop. Dis., 2015, vol. 5, no. 2, pp. 153–157.CrossRefGoogle Scholar
  2. 2.
    Jung, J.-H., Choi, N.-Y., and Lee, S.-Y., Biofilm formation and exopolysaccharide (EPS) production by Cronobacter sakazakii depending on environmental conditions, Food Microbiol., 2013, vol. 1, no. 34, pp. 70–80.CrossRefGoogle Scholar
  3. 3.
    Mirzaee, M., Najar-Peerayeh, S., Behmanesh, M., Moghadam, M.F., and Ghasemian, A.-M., Biofilm formation and presence of ica genes in Staphylococcus aureus isolated from intensive care unit, J. Mazandaran Univ. Med. Sci., 2014, vol. 116, no. 24, pp. 32–40.Google Scholar
  4. 4.
    Kumari, S. and Sarkar, P.K., In vitro model study for biofilm formation by Bacillus cereus in dairy chilling tanks and optimization of clean-in-place (CIP) regimes using response surface methodology, Food Control, 2014, vol. 1, no. 36, pp. 153–158.CrossRefGoogle Scholar
  5. 5.
    Vazquez, V., Liang, X., Horndahl, J.K., Ganesh, V.K., Smeds, E., Foster, T.J., et al., Fibrinogen is a ligand for the Staphylococcus aureus microbial surface components recognizing adhesive matrix molecules (MSCRAMM) bone sialoprotein-binding protein (Bbp), J. Biol. Chem., 2011, vol. 34, no. 286, pp. 29797–29805.CrossRefGoogle Scholar
  6. 6.
    Atshan, S.S., Shamsudin, M.N., Karunanidhi, A., van Belkum, A., Lung, L.T.T., Sekawi, S., et al., Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA), Infect., Genet. Evol., 2013, vol. 1, no. 18, pp. 106–112.CrossRefGoogle Scholar
  7. 7.
    Arciola, C.R., Campoccia, D., Gamberini, S., Baldassarri, L., and Montanaro, L., Prevalence of cna, fnbA and fnbN adhesin genes among Staphylococcus aureus isolates from orthopedic infections associated to different types of implant, FEMS Microbiol. Lett., 2005, vol. 1, no. 246, pp. 81–86.CrossRefGoogle Scholar
  8. 8.
    Arciola, C.R., Campoccia, D., Speziale, P., Montanaro, L., and Costerton, J.W., Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials, Biomaterials, 2012, vol. 26, no. 33, pp. 5967–5982.CrossRefGoogle Scholar
  9. 9.
    Rasmussen, G., Monecke, S., Ehricht, R., and Söderquist, B., Prevalence of clonal complexes and virulence genes among commensal and invasive Staphylococcus aureus isolates in Sweden, PloS One, 2013, vol. 10, no. 8, p. e77477.CrossRefGoogle Scholar
  10. 10.
    Lim, Y., Shin, H.J., Kwon, A.S., Reu, J.H., Park, G., and Kim, J., Predictive genetic risk markers for strong biofilm-forming Staphylococcus aureus: fnbB gene and SCCmec type III, Diagn. Microbiol. Infect. Dis., 2013, vol. 4, no. 76, pp. 539–541.CrossRefGoogle Scholar
  11. 11.
    Rasigade, J.P., Moulay, A., Lhoste, Y., Tristan, A., Bes, M., Vandenesch, F., et al., Impact of subinhibitory antibiotics on fibronectin-mediated host cell adhesion and invasion by Staphylococcus aureus, BMC Microbiol., 2011, vol. 1, no. 11, p. 263.CrossRefGoogle Scholar
  12. 12.
    Atshan, S.S., Nor Shamsudin, M., Sekawi, Z., Lung, L.T.T., Hamat, R.A., Karunanidhi, A., et al., Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus, J. Biomed. Biotechnol., 2012, vol. 2012, id. 976972. doi: 10.1155/2012/976972Google Scholar
  13. 13.
    Vázquez-Sánchez, D., Cabo, M.L., Ibusquiza, P.S., and Rodríguez-Herrera, J.J., Biofilm-forming ability and resistance to industrial disinfectants of Staphylococcus aureus isolated from fishery products, Food Control, 2014, vol. 39, pp. 8–16.CrossRefGoogle Scholar
  14. 14.
    Violante, T.L., Haase, E.M., and Vickerman, M.M., Collagen-binding streptococcal surface proteins influence the susceptibility of biofilm cells to endodontic antimicrobial solutions, J. Endodontics, 2013, vol. 3, no. 39, pp. 370–374.CrossRefGoogle Scholar
  15. 15.
    Arciola, C.R., Campoccia, D., Speziale, P., Montanaro, L., and Costerton, J.W., Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials, Biomaterials, 2012, vol. 26, no. 33, pp. 5967–5982.CrossRefGoogle Scholar
  16. 16.
    Rice, K., Huesca, M., Vaz, D., and McGavin, M.J., Variance in fibronectin binding andfnb locus polymorphisms in Staphylococcus aureus: Identification of antigenic variation in a fibronectin binding protein adhesin of the epidemic CMRSA-1 strain of methicillin-resistant S. aureus, Infect. Immun., 2001, vol. 6, no. 69, pp. 3791–3799.CrossRefGoogle Scholar
  17. 17.
    Wann, E.R., Gurusiddappa, S., and Höök, M., The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus is a bifunctional protein that also binds to fibrinogen, J. Biol. Chem., 2000, vol. 18, no. 275, pp. 13863–13871.CrossRefGoogle Scholar
  18. 18.
    McCourt, J., O’Halloran, D.P., McCarthy, H., O’Gara, J.P., and Geoghegan, J.A., Fibronectin-binding proteins are required for biofilm formation by community-associated methicillin-resistant Staphylococcus aureus strain LAC, FEMS Microbiol. Lett., 2014, vol. 2, no. 353, pp. 157–164.CrossRefGoogle Scholar
  19. 19.
    Arciola, C.R., Bustanji, Y., Conti, M., Campoccia, D., Baldassarri, L., Samorì, B., et al., Staphylococcus epidermidis fibronectin binding and its inhibition by heparin, Biomaerials, 2003, vol. 18, no. 24, pp. 3013–3019.CrossRefGoogle Scholar

Copyright information

© Allerton Press, Inc. 2015

Authors and Affiliations

  • Mohsen Mirzaee
    • 1
  • Shahin Najar-Peerayeh
    • 1
    Email author
  • Mehrdad Behmanesh
    • 2
  1. 1.Department of Bacteriology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of Genetics, School of Biological ScienceTarbiat Modares UniversityTehranIran

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