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Molecular Genetics, Microbiology and Virology

, Volume 23, Issue 3, pp 119–125 | Cite as

Development of a multiplex PCR procedure for detection of Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica)

  • A. M. Stenkova
  • M. P. Isaeva
  • V. A. Rasskazov
Experimental Papers

Abstract

To identify the bacteria of the Yersinia genus and pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) in a single reaction, a multiplex PCR technique, which uses genes of nonspecific porins (OmpF-like proteins), has been developed; it was optimized by five PCR buffer compounds and the temperature of primer annealing. Detection efficiency of genus-and species-specific primers was determined. The multiplex PCR provides an improved rapid technique for detecting the Yersinia genus and identifying pathogenic species.

Keywords

Pathogenic Species Molecular Mass Marker Pantoea Agglomerans Yersinia Species Yersinia Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Allerton Press, Inc. 2008

Authors and Affiliations

  • A. M. Stenkova
    • 1
  • M. P. Isaeva
    • 1
  • V. A. Rasskazov
    • 1
  1. 1.Pacific Institute of Bioorganic Chemistry, Far-East DivisionRussian Academy of Medical SciencesVladivostokRussia

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