Development of a multiplex PCR procedure for detection of Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica)
- First Online:
- 133 Downloads
To identify the bacteria of the Yersinia genus and pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) in a single reaction, a multiplex PCR technique, which uses genes of nonspecific porins (OmpF-like proteins), has been developed; it was optimized by five PCR buffer compounds and the temperature of primer annealing. Detection efficiency of genus-and species-specific primers was determined. The multiplex PCR provides an improved rapid technique for detecting the Yersinia genus and identifying pathogenic species.
Unable to display preview. Download preview PDF.