Molecular Genetics, Microbiology and Virology

, Volume 23, Issue 3, pp 119–125

Development of a multiplex PCR procedure for detection of Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica)

  • A. M. Stenkova
  • M. P. Isaeva
  • V. A. Rasskazov
Experimental Papers

Abstract

To identify the bacteria of the Yersinia genus and pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) in a single reaction, a multiplex PCR technique, which uses genes of nonspecific porins (OmpF-like proteins), has been developed; it was optimized by five PCR buffer compounds and the temperature of primer annealing. Detection efficiency of genus-and species-specific primers was determined. The multiplex PCR provides an improved rapid technique for detecting the Yersinia genus and identifying pathogenic species.

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Copyright information

© Allerton Press, Inc. 2008

Authors and Affiliations

  • A. M. Stenkova
    • 1
  • M. P. Isaeva
    • 1
  • V. A. Rasskazov
    • 1
  1. 1.Pacific Institute of Bioorganic Chemistry, Far-East DivisionRussian Academy of Medical SciencesVladivostokRussia

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