Estimating the Toxicity and Biological Availability for Interaction Products of Metallic Iron and Humic Substances
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This article considers the influence that suspensions of nanoparticles (sized from 10 to 60 nm) of iron oxo-compounds in different oxidation states have on biological objects. The suspension is formed by the interaction of metallic iron with aqueous solutions of humic substances. Based on the example of green microalgae Scenedesmus quadricauda (Turp.) Breb., it is shown that suspensions that contain iron oxo-compound nanoparticles stabilized with humic substances at an iron concentration from 0.14 to 2036 µM do not have a toxic effect on microalgae. The availability of iron contained in the suspensions was evaluated in the experiment on sprouts of wheat Triticum aestivum L., which had been grown under iron-deficient conditions. The root uptake of the ionic form of iron contained in the suspension was confirmed. It is shown that the studied suspensions of iron nanoparticles stabilized by humic substances accumulate on the surface of plant roots. These suspensions are supposed to be a source of iron with prolonged action for plants.
Keywords:humic substances iron-containing nanoparticles iron oxo-compounds iron containing preparations corrosion evaluation of biological availability toxicological evaluation
We are grateful to T.B. Egorova for analysis of the preparations by transmission electron microscopy.
This study was supported by the Russian Scientific Foundation, project no. 16-14-00167.
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interests. The authors declare that they have no conflicts of interest.
Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.
- 1.Anuchina, M.M., Badun, G.A., Severin, A.V., et al., Sorption of humic matter marked by tritium at inorganic sorbents, Materialy II Vserossiiskoi nauchnoi konferentsii “Aktual’nye problemy adsorbtsii i kataliza” (Proc. II All-Russian Sci. Conf. “Topical Problems on Adsorption and Catalysts”), Ples, 2017.Google Scholar
- 2.Anuchina, M.M. and Shakirov, S.M., Role of humic matters in forming nano-sized particles of corrosion products of metallic iron, Materialy XXIV Mezhdunarodnoi nauchnoi konferentsii studentov, aspirantov i molodykh uchenykh “Lomonosov-2017” (Proc. XXIV Int. Sci. Conf. for Students, Postgraduates and Young Scientists “Lomonosov-2017”), Moscow, 2017. https://lomonosovmsu.ru/archive/Lomonosov_2017/ data/section_12_10955.htm. Cited 28.05.2018.Google Scholar
- 3.Gaisina, L.A., Fazlutdinova, A.I., and Kabirov, R.R., Sovremennye metody vydeleniya i kul’tivirovaniya vodoroslei (Modern Methods for Algae Extracting and Cultivating), Ufa, 2008.Google Scholar
- 4.Zhmur, N.S. and Orlova, T.L., Metodika opredeleniya toksichnosti vod, vodnykh vytyazhek iz pochv, osadkov stochnykh vod i otkhodov po izmeneniyu urovnya fluorestsentsii khlorofilla i chislennosti kletok vodoroslei (Methods for Determining Toxicity of Water, Water Extracts from Soils, Waste Water Sediments and Wastes according to f Chlorophyll Fluorescence Variation and Algae Cells Number), Moscow, 2001.Google Scholar
- 5.Kudryavtseva, E.A., Anilova, A.V., Kuz’min, S.N., and Sharygina, M.V., Correlation between different forms of ferrum and Triticum aestivum L. seeds sprouting, Vestn. Orenb. Gos. Univ., 2013, no. 6 (155).Google Scholar
- 6.Marczenko, Z. and Balcerzak, M., Separation, Preconcentration, and Spectrophotometry in Inorganic Analysis, Amsterdam: Elsevier, 2001.Google Scholar
- 7.Matorin, D.N. and Rubin, A.B., Fluorestsentsiya khlorofilla vysshikh rastenii i vodoroslei (Fluorescence of Chlorophyll of Higher Plants and Algae), Moscow, 2012.Google Scholar
- 8.Pankratov, D.A., Sorkina, T.A., Karelina, E.E., et al., The way to simulate iron redox transformations in organic-inorganic compounds with humic matters, Materialy XII Mezhdunarodnoi konferentsii “Messbauerovskaya spektroskopiya i ee primeneniya” (Proc. XII Int. Conf. “Mössbauer Spectroscopy and Its Applications”), Suzdal, Moscow, 2012.Google Scholar
- 9.Sorkina, T.A., Kulikova, N.A., Filippova, O.I., et al., Iron deficiency correctors for plants based on carbon humic matters: synthesizing and application, Ekol. Prom. Rossii, 2010, no. 2.Google Scholar
- 10.Fedoseeva, E.V., Sapunkova, N.Yu., and Terekhova, V.A., Prakticheskaya ekotoksikologiya: otsenka chuvstvitel’nosti biotest-kul’tur (Practical Ecological Toxicology: the Way to Estimate Biotest-Cultures Sensitivity), Moscow, 2016.Google Scholar
- 12.Laurent, S., Forge, D., Port, M., et al., Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications, Chem. Rev., 2008, vol. 108, no. 6.Google Scholar
- 17.Pourbaix, M., Thermodynamics and corrosion, Corros. Sci., 1990, vol. 3, no. 10.Google Scholar
- 19.Rosei, F., Nanostructured surfaces: challenges and frontiers in nanotechnology, J. Phys. Condens. Matter, 2004, vol. 16, no. 17.Google Scholar