Study of hydrocarbon oxidizing microorganisms from deep groundwater of the Puchezh-Katunki impact structure

  • G. V. Kondakova
  • N. V. Verkhovtseva
  • S. A. Ostroumov


The presence of viable hydrocarbon-oxidizing microorganisms has been shown in the under-ground waters exposed by the Vorotilovskaya deep well (the Puchezh-Katunki astrobleme, 75 km north of Nizhny Novgorod, 1900- and 3200-m deep) using the method of chromatography-mass spectrometry of specific biomarkers of the microbial cell wall and the classical methods of bacteriology. Several microbial species have been isolated in pure culture and identified. Two bacillary species, Bacillus pumilus KTB-2 and Bacillus subtilis KTB-4, were maintained in pure cultures at reinoculations. The effects of mineralization and aeration of the medium on the growth characteristics of Bacillus pumilus KTB-2 in batch culture have been studied.


deep underground waters hydrocarbon-oxidizing microorganisms Bacillus pumilus high mineralization of the medium kinetic parameters of growth of microorganisms 


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© Allerton Press, Inc. 2013

Authors and Affiliations

  • G. V. Kondakova
    • 1
  • N. V. Verkhovtseva
    • 2
  • S. A. Ostroumov
    • 2
  1. 1.Demidov Yaroslavl State UniversityYaroslavlRussia
  2. 2.Moscow State UniversityMoscowRussia

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