Hydrogen peroxide, one of the main factors of Mycoplasma pneumoniae pathogenicity, is released in the reaction catalyzed by glycerol-3-phosphate (G3P) oxidase enzyme (EC 126.96.36.199). During the study, nucleotide substitutions in the glycerol-3-phosphate oxidase gene of Mycoplasma pneumoniae clinical isolates, the presence of which was accompanied by different levels of hydrogen peroxide production and cytotoxicity of Mycoplasma pneumoniae isolates regarding respiratory epithelial cells, were detected.
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Saraya, T., Kurai, D., Nakagaki, K., Sasaki, Y., Niwa, S., Tsukagoshi, H., Nunokawa, H., Ohkuma, K., Tsujimoto, N., Hirao, S., Wada, H., Ishii, H., Nakata, K., Kimura, H., Kozawa, K., Takizawa, H., and Goto, H., Novel aspects on the pathogenesis of Mycoplasma pneumoniae and therapeutic implications, Front Microbiol., 2014, vol. 5, article 410.
Elkhal, C.K., Kean, K.M., Parsonage, D., Maenpuen, S., Chaiyen, P., Claiborne, A., and Karpus, P.A., Structure and proposed mechanism of L-glycerophosphate oxidase from Mycoplasma pneumoniae,FEBS J., 2015, vol. 282, pp. 3030–42.
Balish, M.F. and Distelhorst, S.L., Potential molecular targets for narrow-spectrum agents to combat Mycoplasma pneumoniae infection and disease, Front Microbiol., 2016, vol. 7, article 205.
Großhennig, S., Schmidl, S.R., Schmeisky, G., Busse, J., and Stülke, J., Implication of glycerol and phospholipid transporters in Mycoplasma pneumonia growth and virulence, Infect. Immun., 2013, vol. 81, no. 3, pp. 896–904.
Wang, M., Wang, Y., Yan, Y., Zhu, C., Huang, L., Shao, X., Xu, J., Zhu, H., Sun, X., Ji, W., and Chen, Z., Clinical and laboratory profiles of refractory Mycoplasma pneumoniae pneumonia in children, Int. J. Infect. Dis., 2014, vol. 29, pp. 18–23.
Willner, D., Daly, J., Whiley, D., Grimwood, K., Wainwright, C., and Hugenholtz, Ph., Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar lavage samples, PLoS One, 2012, vol. 7, no. 4. https://doi.org/10.1371/journal.pone.003460.5
Mitroshina, E.V., Mischenko, T.A., and Vedunova, M.V., Detection of Viability of Cell Cultures, Nizhni Novgorod: Lobachevsky State University of Nizhni Novgorod, 2015.
Schmidl, S.R., Otto, A., Lluch-Senar, M., Pinol, J., Busse, J., Becher, D., and Stülke, J., A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression, PLoS Pathog., 2011, vol. 9, no. 7. https://doi.org/10.1371/journal.ppat.1002263
Li, S., Li, X., Wang, Y., Yang, J., Chen, Z., and Shan, S., Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection, BMC Microbiol., 2014, vol. 14, article 27.
This study was not supported by any specific grant from funding agencies in the state, commercial, or nonprofit sectors.
Conflict of interest. The authors declare that they have no conflict of interest.
Statement of compliance with standards of research involving humans as subjects. All procedures performed in the study involving human participants were in accordance with the ethical standards of the institutional and/or national research ethics committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.
Translated by A. Barkhash
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Hlinkina, T.V., Kastsiuk, S.A. Correlation between Nucleotide Substitutions in Glycerol-3-Phosphate Oxidase Gene, the Level of Hydrogen Peroxide Production, and Cytotoxicity of Mycoplasma pneumoniae. Cytol. Genet. 54, 18–22 (2020). https://doi.org/10.3103/S0095452720010053
- Mycoplasma pneumoniae
- glycerol-3-phosphate oxidase
- nucleotide substitutions
- hydrogen peroxide