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Correlation between Nucleotide Substitutions in Glycerol-3-Phosphate Oxidase Gene, the Level of Hydrogen Peroxide Production, and Cytotoxicity of Mycoplasma pneumoniae

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Abstract—

Hydrogen peroxide, one of the main factors of Mycoplasma pneumoniae pathogenicity, is released in the reaction catalyzed by glycerol-3-phosphate (G3P) oxidase enzyme (EC 1.1.3.21). During the study, nucleotide substitutions in the glycerol-3-phosphate oxidase gene of Mycoplasma pneumoniae clinical isolates, the presence of which was accompanied by different levels of hydrogen peroxide production and cytotoxicity of Mycoplasma pneumoniae isolates regarding respiratory epithelial cells, were detected.

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Funding

This study was not supported by any specific grant from funding agencies in the state, commercial, or nonprofit sectors.

Author information

Correspondence to T. V. Hlinkina.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in the study involving human participants were in accordance with the ethical standards of the institutional and/or national research ethics committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

Additional information

Translated by A. Barkhash

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Hlinkina, T.V., Kastsiuk, S.A. Correlation between Nucleotide Substitutions in Glycerol-3-Phosphate Oxidase Gene, the Level of Hydrogen Peroxide Production, and Cytotoxicity of Mycoplasma pneumoniae. Cytol. Genet. 54, 18–22 (2020). https://doi.org/10.3103/S0095452720010053

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Keywords:

  • Mycoplasma pneumoniae
  • glycerol-3-phosphate oxidase
  • nucleotide substitutions
  • hydrogen peroxide
  • cytotoxicity