Cytology and Genetics

, Volume 52, Issue 4, pp 269–275 | Cite as

Peculiarities of Regeneration and Genetic Variability of Crambe koktebelica and Crambe tataria Plants in vitro

  • N. O. PushkarovaEmail author
  • O. R. Lakhneko
  • V. B. Belokurova
  • B. V. Morgun
  • M. V. Kuchuk


The regenerative capability of three types of explants was studied on media with different compositions of growth regulators with the purpose of selecting optimal conditions of fast reproduction of endangered Crambe species that could be used as a relevant source of genetic material for the improvement of industrially valuable plants. PCR-analysis of genotypes of C. koktebelica and C. tataria plants was conducted to identify the influence of in vitro cultivation on the genetic stability of plants. The highest regeneration rates were observed with the use of petiole explants on MS medium with BA and NAA. The absence of somaclonal variability in C. koktebelica and C. tataria in vitro regenerated plants was demonstrated.


microclonal propagation in vitro culture DNA-markers Crambe koktebelica Crambe tataria 


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  1. 1.
    Lazzeri, L., De Mattei, F., Bucelli, F., and Palmieri, S., Crambe oil—a potential new hydraulic oil and quenchant, Ind. Lubr. Tribol., 1997, vol. 49, no. 2, pp. 71–77.CrossRefGoogle Scholar
  2. 2.
    Branca, F. and Cartea, E., Brassica, in Wild Crop Relatives: Genomic and Breeding Resources, vol. V: Oilseeds, Kole, C., Ed., Heidelberg: Springer-Verlag, 2011, pp. 17–36.CrossRefGoogle Scholar
  3. 3.
    Goncalves, A.C.Jr., Rubio, F., Meneghel, A.P., Coelho, G.F., Dragunski, D.C., and Strey, L., The use of Crambe abyssinica seeds as adsorbent in the removal of metals from waters, Rev. Bras. Eng. Agric. Ambient, 2013, vol. 17, no. 3, pp. 306–311.CrossRefGoogle Scholar
  4. 4.
    Didukh, Ya.P., Crambe koktebelika, in Red Data Book of Ukraine, Plant Kingdom, Kyiv: Globalconsulting, 2009, p. 360.Google Scholar
  5. 5.
  6. 6.
    Bowes, B.G., In vitro morphogenesis of Crambe maritime L., Protoplasma, 1976, vol. 89, no. 1, pp. 185–188.CrossRefGoogle Scholar
  7. 7.
    Piovan, A., Cassina, G., and Filippini, R., Crambe tataria: actions for ex situ conservation, Biodivers. Conserv., 2011, vol. 20, no. 2, pp. 359–371.CrossRefGoogle Scholar
  8. 8.
    Twardovska, M.O., Drobyk, N.M., Melnyk, V.M., Konvalyuk, I.I., and Kunakh, V.A., Genome variability of some Gentiana L. species in nature and in culture in vitro: RAPD-analysis, Biopolym. Cell, 2010, vol. 26, no. 6, pp. 499–507.CrossRefGoogle Scholar
  9. 9.
    Nehra, N.S., Kartha, K.K., Stushnott, C., and Giles, K.L., The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry, Plant Cell Tissue Organ Cult., 1992, vol. 29, no. 3, pp. 257–268.CrossRefGoogle Scholar
  10. 10.
    Kumar, P.S. and Mathur, V.L., Chromosomal instability in callus culture of Pisum sativum, Plant Cell Tissue Organ Cult., 2004, vol. 78, pp. 267–271.CrossRefGoogle Scholar
  11. 11.
    Mosula, M.Z. and Konvalyuk, I.I., Mel’nyk, V.M., Drobyk, N.M., Tsaryk, Y.V., Nesteruk, Yu.Y., and Kunakh, V.A., Genetic polymorphism of Gentiana lutea L. (Gentianaceae) populations from the Chornohora Ridge of the Ukrainian Carpathians, Cytol. Genet., 2014, vol. 48, no. 6, pp. 371–377.CrossRefGoogle Scholar
  12. 12.
    Bublyk, O.M., Andreyev, I.O., Spiridonova, K.V., and Kunakh, V.A., The variability of morphogenic and nonmorphogenic tissue culture of Ungernia victoris by the results of RAPD analysis, Visn. Ukr. Tov. Genet. Sel., 2008, vol. 6, no. 1, pp. 44–51.Google Scholar
  13. 13.
    Belokurova, V.B., Lystvan, K.V., Maystrov, P.D., Sikura, Y.Y., Gleba, Yu.Yu., and Kuchuk, N.V., Using methods of plant biotechnology for conservation and study of the world flora biodiversity, Cytol. Genet., 2005, vol. 39, no. 1, pp. 41–51.Google Scholar
  14. 14.
    Pushkarova, N.O., Belokurova, V.B., and Kuchuk, M.V., Seed surface sterilization efficiency as an important prerequisite in formation of endangered plant species in vitro collections, in Factors in Experimental Evolution of Organisms, 2015, vol. 17, pp. 241–244.Google Scholar
  15. 15.
    Lowe, A.J., Jones, A.E., Raybould, A.F., Trick, M., Moule, C.L., and Edwards, K.J., Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle, Mol. Ecol. Res., 2002, vol. 2, no. 1, pp. 7–11.Google Scholar
  16. 16.
    Werner, E.T., Soares, T.C., Gontijo, A.B., Souza, Neto J.D., and Amaral, J.A., Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers, Genet. Mol. Res., 2015, vol. 14, no. 4, pp. 16450–16460.CrossRefPubMedGoogle Scholar
  17. 17.
    Khalil, R.M.A., Soliman, Kh.A., Rashed, N.A.K.F., and Ibrahim, S.A., Genetic polymorphism of some medicinal plants belonging to Brassicaceae using molecular markers, Egypt. J. Genet. Cytol., 2010, vol. 39, no. 1, pp. 41–55.Google Scholar
  18. 18.
    Singhal, H., Ren, Y.R., and Kern, S.E., Improved DNA electrophoresis in conditions favoring polyborates and Lewis acid complexation, PLoS One, 2010, vol. 5, no. 6. e11318.CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Brody, J.R. and Kern, S.E., History and principles of conductive media for standard DNA electrophoresis, Anal. Biochem., 2004, vol. 333, no. 1, pp. 1–13.CrossRefPubMedGoogle Scholar
  20. 20.
    Sambrook, J. and Russell, D.W., Molecular Cloning: A Laboratory Manual, New York: CSHL Press, 2001.Google Scholar
  21. 21.
    Perrier, X., Flori, A., and Bonnot, F., Data analysis methods, in Genetic Diversity of Cultivated Tropical Plants, Hamon, P., Seguin, M., Perrier, X., and Glaszmann, J.C., Eds., Enfield: Sci. Publ., 2003, pp. 43–76.Google Scholar
  22. 22.
  23. 23.
    Perrier, X. and Jacquemoud-Collet, J.P., DARwin software, 2006. Google Scholar
  24. 24.
    Lakin, G.F., Biometry, Moscow: Vysshaya shkola, 1990.Google Scholar
  25. 25.
    Pathak, H. and Dhawan, V., ISSR assay for ascertaining genetic fidelity of micropropagated plants of apple rootstock Merton 793, In Vitro Cell. Dev. Biol. Plant., 2012, vol. 48, no. 1, pp. 137–143.CrossRefGoogle Scholar
  26. 26.
    Tarikahya-Hacioglu, B., Molecular diversity of the wild Crambe (Brassicaceae) taxa in Turkey detected by inter-simple sequence repeats (ISSRs), Ind. Crops Prod., 2015, vol. 80, pp. 214–219.CrossRefGoogle Scholar

Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • N. O. Pushkarova
    • 1
    Email author
  • O. R. Lakhneko
    • 1
  • V. B. Belokurova
    • 1
  • B. V. Morgun
    • 1
  • M. V. Kuchuk
    • 1
  1. 1.Institute of Cell Biology and Genetic EngineeringNational Academy of Sciences of UkraineKyivUkraine

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