Cytology and Genetics

, Volume 50, Issue 5, pp 324–329 | Cite as

Production of branched root hairs under progressive drought stress in Arabidopsis thaliana



In this study, data on the production of branched root hairs in the seedlings of A. thaliana under progressive water deficit were presented. The overall production of branched hairs was quite high under stress conditions and amounted to 8.27%. On the contrary, this form of root hairs was almost absent in the control group (0.27%). The highest number of branched hairs was produced at the beginning of the stress action. Branched root hairs are quite uniform structures in the sense of their morphology. To solve the question of how the branched hairs grow, the structure of actin cytoskeleton was explored. This structure was different in the root hair and in its branch, which is an indication that the hair stops its growth at the moment when the branching starts. We have also characterized the production of branched root hairs in hormonal mutants of Arabidopsis and found the involvement of auxin in this process.


Arabidopsis thaliana progressive water stress branched root hairs actin cytoskeleton auxin 


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  1. 1.
    Seki, M., Narusaka, M., Abe, H., Kasuga, M., Yamaguchi-Shinozaki, K., Carninci, P., Hayashizaki, Y., and Shinozaki, K., Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray, Plant Cell, 2001, vol. 13, no. 1, pp. 61–72.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., and Shinozaki, K., Improving plant drought, salt and freezing tolerance by gene transfer of a single stress- NH4+ inducible transcription factor, Nature Biotechnol., 1999, vol. 17, pp. 287–291.CrossRefGoogle Scholar
  3. 3.
    Söderman, E., Hjellström, M., Fahleson, J., and Engström, P., The HD-Zip gene ATHB6 in Arabidopsis is expressed in developing leaves, roots and carpels and up-regulated by water deficit conditions, Plant Mol. Biol., 1999, vol. 40, no. 6, pp. 1073–1083.CrossRefPubMedGoogle Scholar
  4. 4.
    Huang, D., Wu, W., Abrams, S.R., and Cutler, A.J., The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors, J. Exp. Bot., 2008, vol. 59, no. 11, pp. 2991–3007.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Bobrownyzky, J., A method for the imitation of drought stress in Arabidopsis thaliana (L.) Heynh., Plant Introduc., 2006, no. 1, pp. 98–105.Google Scholar
  6. 6.
    Mueller, M. and Schmidt, W., Environmentally induced plasticity of root hair development in Arabidopsis, Plant Physiol., 2004, vol. 134, no. 1, pp. 409–419.CrossRefGoogle Scholar
  7. 7.
    Duzan, H.M., Zhou, X., Souleimanov, A., and Smith, D.L., Perception of Bradyrhizobium japonicum Nod factor by soybean [Glycine max (L.) Merr.] root hairs under abiotic stress conditions, J. Exp. Bot., 2004, vol. 55, no. 408, pp. 2641–2646.CrossRefPubMedGoogle Scholar
  8. 8.
    Ketelaar, T., De Ruijter, N.C., and Emons, A.M., Unstable F-actin specifies the area and microtubule direction of cell expansion in Arabidopsis root hair, Plant Cell, 2003, vol. 15, no. 1, pp. 285–292.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Ketelaar, T., Faivre-Moskalenko, C., Esseling, J.J., a de Ruijter, N.C., and Grierson, C.S., Dogterom M., Emons A.M.C., Positioning of nuclei in Arabidopsis root hairs: an actin-regulated process of tip growth, Plant Cell, 2002, vol. 14, no. 11, pp. 2941–2955.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Shaw, S.L. and Long, S.R., Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells, Plant Physiol., 2003, vol. 131, no. 3, pp. 976–984.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Baskin, T.I. and Wilson, J.E., Inhibitors of protein kinases and phosphatases alter root morphology and disorganize cortical microtubules, Plant Physiol., 1997, vol. 113, no. 2, pp. 493–502.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Van der Weele, C.M., Spollen, W.G., Sharp, R.E., and Basin, T.I., Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient-agar media, J. Exp. Bot., 2000, vol. 51, no. 350, pp. 1555–1562.CrossRefPubMedGoogle Scholar
  13. 13.
    Miller, D.D., De Ruijter, N.C.A., Bisseling, T., and Emons, A.M.C., The role of actin in root hair morphogenesis: studies with lipochito-oligosaccharide as a growth stimulator and cytochalasin as an actin perturbing drug, Plant J., 1999, vol. 17, no. 2, pp. 141–154.CrossRefGoogle Scholar
  14. 14.
    Ringli, C., Baumberger, N., Diet, A., Frey, B., and Keller, B., ACTIN2 is essential for bulge site selection and tip growth during root hair development of Arabidopsis, Plant Physiol., 2002, vol. 129, no. 4, pp. 1464–1472.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Schiefelbein, J.W., Constructing a plant cell: the genetic control of root hair development, Plant Physiol., 2000, vol. 124, no. 4, pp. 1525–1531.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Peterson, R.L. and Stevens, K.J., Evidence for the uptake of non-essential ions and essential nutrient ions by root hairs and their effect on root hair development, in Root Hairs. Cell and Molecular Biology, Ridge, R.W. and Emons, A.M.C., Eds., Tokyo: Springer, 2000, pp. 179–195.CrossRefGoogle Scholar
  17. 17.
    Schmidt, W., Tittel, J., and Schikora, J., Role of hormones in the induction of iron deficiency responses in Arabidopsis roots, Plant Physiol., 2000, vol. 122, no. 4, pp. 1109–1118.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Uphof, J.C. and Hummer, K., Encyclopedia of Plant Anatomy, vol. 5: Plant Hairs, Zimmerman, W. and Ozenda, P.G., Eds., Berlin, 1962.Google Scholar
  19. 19.
    Vasellati, V., Oesterheld, M., Medan, D., and Loreti, J., Effects of flooding and drought on the anatomy Paspalum dilatatum, Ann. Bot., 2001, vol. 88, no. 3, pp. 355–360.CrossRefGoogle Scholar
  20. 20.
    Guimi, S. and Dunand, C., Cell growth and differentiation in Arabidopsis epidermal cells, J. Exp. Bot., 2007, vol. 58, no. 14, pp. 3829–3840.CrossRefGoogle Scholar
  21. 21.
    Yang, N., Zhu, C., Gan, L., Ng, D., and Xia, K., Ammonium-stimulated root hair branching is enhanced by methyl jasmonate and suppressed by ethylene in Arabidopsis thaliana, J. Plant Biol., 2011, vol. 54, no. 2, pp. 92–100.CrossRefGoogle Scholar
  22. 22.
    Zhu, C., Gan, L., Shen, Z., and Xia, K., Interactions between jasmonates and ethylene in the regulation of root hair development in Arabidopsis, J. Exp. Bot., 2006, vol. 57, no. 6, pp. 1299–1308.CrossRefPubMedGoogle Scholar
  23. 23.
    Thimm, O., Essigmann, B., Kloska, S., Altmann, T., and Buckhout, T.J., Response of Arabidopsis to iron deficiency stress as revealed by microarray analysis, Plant Physiol., 2001, vol. 127, no. 3, pp. 1030–1043.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Wilson, A.K., Pickett, F.B., Turner, J.C., and Estelle, M., A dominant mutation in Arabidopsis confers resistance to auxin, ethylene, and abscisic acid, Mol. Gen. Genet., 1990, vol. 222, nos. 2–3, pp. 377–383.CrossRefPubMedGoogle Scholar
  25. 25.
    Leyser, H.M.O., Pickett, F.B., Dharmasiri, S., and Estelle, M., Mutations in the AXR3 gene of Arabidopsis result in altered auxin responses including ectopic expression of the SAUR-AC1 promoter, Plant J., 1996, vol. 10, no. 3, pp. 403–413.CrossRefPubMedGoogle Scholar

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© Allerton Press, Inc. 2016

Authors and Affiliations

  1. 1.Institute of BotanyNational Academy of Sciences of UkraineKyivUkraine

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