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Cytology and Genetics

, Volume 48, Issue 5, pp 302–307 | Cite as

Plant regeneration from hairy roots and calluses of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L.

  • L. G. Lioshina
  • O. V. Bulko
Article

Abstract

Plants regenerated from hairy root culture and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with varying efficiency. The frequency of direct shoot formation from root cultures was up to 60% in Digitalis and 3.7% in Vinca. The addition of 1 mg/L BA, 0.1 mg/L NAA, and 5% sucrose to B5 medium increases regenerative capacity of periwinkle up to 19.1%. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology, and short internodes.

Keywords

hairy roots plant regeneration Vinca minor L. Digitalis purpurea L. 

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Copyright information

© Allerton Press, Inc. 2014

Authors and Affiliations

  1. 1.Institute of Bioorganic Chemistry and PetrochemistryNational Academy of Sciences of UkraineKyivUkraine

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