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The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers

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Moscow University Chemistry Bulletin Aims and scope

Abstract—An immunochromatographic test system for the combined analysis of two cardiomarkers, such as fatty acid binding protein (FABP) and troponin I (TnI), in serum and whole blood is developed. The main stages of the design of a multiparametric system are investigated, including the influence of the antibody position and gold nanoparticle size on the results. The detection limits of the method are found to be 10 ng/mL for FABP and 1 ng/mL for TnI, and the coefficient of variation did not exceed 10%. The developed test system allows us to confirm the diagnosis of acute myocardial infraction (N = 9). The analysis time is 15 min.

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Funding

This work was supported by the Ministry of Education and Science as part of the state task “Organization of research” (agreement no. 16.6548.2017/VU).

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, Russia

    I. P. Andreeva, E. A. Yakovleva, V. G. Grigorenko & A. P. Osipov

  2. National Research Technological University MISiS, Moscow, Russia

    A. P. Osipov

Authors
  1. I. P. Andreeva
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  2. E. A. Yakovleva
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  3. V. G. Grigorenko
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  4. A. P. Osipov
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Corresponding author

Correspondence to A. P. Osipov.

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The authors declare that they have no conflict of interest.

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Translated by O. Maslova

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Andreeva, I.P., Yakovleva, E.A., Grigorenko, V.G. et al. The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers. Moscow Univ. Chem. Bull. 74, 315–321 (2019). https://doi.org/10.3103/S002713141906004X

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  • DOI: https://doi.org/10.3103/S002713141906004X

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