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The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers

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Abstract—An immunochromatographic test system for the combined analysis of two cardiomarkers, such as fatty acid binding protein (FABP) and troponin I (TnI), in serum and whole blood is developed. The main stages of the design of a multiparametric system are investigated, including the influence of the antibody position and gold nanoparticle size on the results. The detection limits of the method are found to be 10 ng/mL for FABP and 1 ng/mL for TnI, and the coefficient of variation did not exceed 10%. The developed test system allows us to confirm the diagnosis of acute myocardial infraction (N = 9). The analysis time is 15 min.

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Funding

This work was supported by the Ministry of Education and Science as part of the state task “Organization of research” (agreement no. 16.6548.2017/VU).

Author information

Correspondence to A. P. Osipov.

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The authors declare that they have no conflict of interest.

Additional information

Translated by O. Maslova

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Andreeva, I.P., Yakovleva, E.A., Grigorenko, V.G. et al. The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers. Moscow Univ. Chem. Bull. 74, 315–321 (2019). https://doi.org/10.3103/S002713141906004X

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Keywords:

  • immunochromotographic analysis
  • fatty acid binding protein
  • troponin I
  • acute myocardial infraction