Stabilization of enzymes-antioxidants by complex and conjugate formation with block copolymers: Prospects for CNS treatment
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Nanosized particles with a radius of 16 ± 2 nm based on complexes and conjugates of highly active superoxide dismutase and catalase with polyelectrolyte block copolymer poly(ethyleneimine)-poly(ethylene glycol) and similar bienzyme systems were obtained. Mass spectrometry was used to confirm the crosslinking of enzyme and block copolymer molecules in the nanoparticles that were formed. A significant increase of SOD and catalase stability (up to four times) towards proteolytic degradation under chymotrypsin and trypsin action for 3 h at 37°C was revealed when enzyme-containing nanoparticles were used for experiments. Antioxidant enzymes-containing polyelectrolyte nanoparticles seem to be promising for BBB penetration and CNS drug delivery.
Key wordsantioxidant enzymes superoxide dismutase and catalase bienzyme system nanoparticles neuro-degenerative diseases polyelectrolyte enzyme stabilization
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