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Assessment of selected B cells populations in the workers of X-ray departments

  • Original Paper
  • Published:
International Journal of Occupational Medicine and Environmental Health

Abstract

Objectives

Workers of X-ray departments are occupationally exposed to long-term low levels of ionizing radiation (LLIR), which may affect their humoral immunity. The aim of the study was to assess the influence of LLIR on the number and proportion of B cells (CD19+), B1 cells (CD5+CD19+) and memory B cells (CD27+CD19+) in peripheral blood of such workers.

Materials and Methods

In the study group of 47 X-ray departments workers and the control group consisting of 38 persons, the number and percentage of CD19+, CD5+CD19+, CD27+CD19+ cells as well as CD5+CD19+/CD19+ and CD27+CD19+/CD19+ cell ratios were assessed using flow cytometry. Additionally, the study group was divided into 2 groups by the length of employment below and over 15 years and analysis adjusted for age and smoking habit was performed.

Results

The total number of CD19+ cells showed significant increase in the group of workers in comparison with the persons from the control group, whereas the percentage of CD5+CD19+ cells as well as CD27+CD19+/CD19+ and CD5+CD19+/CD19+ cell ratios were lower. Percentage, number of CD5+CD19+ cells and CD5+CD19+/CD19+ cell ratio were significantly lower in the workers with length of employment longer than 15 years in comparison with those employed below 15 years. Moreover, we found positive associations between the number of CD19+ cells and employment as well as smoking habit, whereas the number of CD5+CD19+ cells was positively associated with cigarette smoking alone. Percentage of CD5+CD19+ cells as well as CD5+CD19+/CD19+ and CD27+CD19+/CD19+ cell ratios were negatively correlated with employment.

Conclusions

The study suggests association between the suppressive influence of low level ionizing radiation on circulating in peripheral blood, especially of B1 cells as well as of memory B cells, in workers of X-ray units, which is adverse in relation to microbiological threat.

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Medical University of Silesia, Katowice, Poland

    Piotr Kłuciński, Małgorzata Aptekorz & Gayane Martirosian

  2. Department of Microbiology and Immunology, Medical University of Silesia, Zabrze, Poland

    Bogdan Mazur

  3. Department and Clinic of Pediatrics Hematology and Oncology, Medical University of Silesia, Zabrze, Poland

    Łukasz Sędek

  4. Department and Clinic of Internal, Autoimmune and Metabolic Diseases, Medical University of Silesia, Katowice, Poland

    Paweł Cieślik & Antoni H. Hrycek

  5. Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752, Katowice, Poland

    Piotr Kłuciński

Authors
  1. Piotr Kłuciński
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  2. Bogdan Mazur
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  3. Łukasz Sędek
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  4. Małgorzata Aptekorz
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  5. Paweł Cieślik
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  6. Antoni H. Hrycek
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  7. Gayane Martirosian
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Corresponding author

Correspondence to Piotr Kłuciński.

Additional information

This study was supported by the Medical University of Silesia in Katowice and prepared as part of the research project no. KNW-2-090/10. Study manager: Piotr Kłuciński, MD, Associate Professor.

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Kłuciński, P., Mazur, B., Sędek, Ł. et al. Assessment of selected B cells populations in the workers of X-ray departments. IJOMEH 27, 467–473 (2014). https://doi.org/10.2478/s13382-014-0242-3

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  • DOI: https://doi.org/10.2478/s13382-014-0242-3

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