Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice

Translational Neuroscience volume 5, pages 57–63 (2014)Cite this article

Abstract

Background

A small dose of the Bordetella pertussis vaccine is used as an adjuvant for the induction of experimental autoimmune encephalomyelitis (EAE) in mice. The effects of two doses of the Pertussis vaccine on clinical signs, antibody titers, and the expression of CD4 and MHC molecules in brain tissue sections of mice with EAE were examined.

Methodology

EAE was induced by spinal cord homogenate in Complete Freund adjuvant (CFA) in 30 of 40 C57BL/6 mice divided in groups: EAE mice with a small adjuvant dose of the Pertussis vaccine (EAE-1), EAE mice with a human dose of the Pertussis vaccine (EAE-2), EAE mice (EAE-3).

Results

None of the mice from the EAE groups progressed to severe EAE. Five mice from the EAE-2 group were found dead on the 13th day post-immunization. A significant increase of anti-MOG (myelin oligodendrocyte glycoprotein) antibodies was detected in mice with EAE compared to non-treated mice. Myelin loss and brain tissue lesions were observed in EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A high expression of MHC-II and a mild expression of MHC-I was detected in the brains of mice with EAE. No expressions were detected in intact brains. Scattered CD4-positive cells were detected in the brains of EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice.

Conclusion

A small dose of the Bordetella pertussis vaccine could maintain the developed clinical signs and histological changes in mice with EAE, while higher doses led to additional adverse effects. The expression of CD4 and MHC class I and II molecules, as well as an increase in anti-MOG antibodies could be used as markers capable of monitoring the development and progression of EAE.

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Author information

Affiliations

  1. Faculty of Pharmacy, University of Bijeljina, Pavlovića put bb, Bijeljina, Republika Srpska Bosnia and Herzegovina

    Aleksandra Stojković

  2. Biomedical Center of the Institute Torlak, 11000, Belgrade, Serbia

    Irina Maslovarić & Dejana Kosanović

  3. Institute for Transfusiology and Hemobiology of the Military Medical Academy, Crnotravska 17, Belgrade, Serbia

    Dušan Vučetić

  4. Faculty of Medicine of the Military Medical Academy, University of Defense, Crnotravska 17, Belgrade, Serbia

    Dušan Vučetić

Authors
  1. Aleksandra Stojković
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  2. Irina Maslovarić
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  3. Dejana Kosanović
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  4. Dušan Vučetić
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Correspondence to Aleksandra Stojković.

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Stojković, A., Maslovarić, I., Kosanović, D. et al. Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice. Translat.Neurosci. 5, 57–63 (2014). https://doi.org/10.2478/s13380-014-0206-x

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Keywords

  • Autoimmunity
  • EAE
  • MHC molecules
  • Pertussis vaccine