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Biologia

pp 1–12 | Cite as

Modulatory effect of Ficus carica on oxidative stress and hematological changes induced by gamma-radiation in male albino rats

  • Dalia FouadEmail author
  • Eman Al-Obaidi
  • Amira Badr
  • Farid S. Ataya
  • Rewaida Abdel-Gaber
Original Article
  • 37 Downloads

Abstract

The fig, Ficus carica L. (Moraceae), is a rich source of polysaccharides that possessed anti-tumour and anti-oxidant properties. The present study aimed to evaluate the ability of F. carica to protect against radiation-induced changes in certain biochemical and hematological parameters. This was achieved by measuring different hematological parameters, antioxidant enzyme activities, and lipid peroxidation; histological examination of liver and kidney was also performed. Rats used in this study were divided into four groups of 10 each- group 1: control, group 2: F. carica, group 3: irradiated rats, and group 4:F. carica pretreated irradiated rats. Ficus carica extract was prepared in water in a 1:3 w:v ratio and administered by gavage for three consecutive weeks before whole body gamma irradiation with 8 Gy (single dose). Five rats were sacrificed from each group at 24 and 72 h after radiation exposure. Irradiation resulted in marked reduction in white blood cell (WBC), platelets (PLT), lymphocyte, and neutrophil counts, whereas no significant changes were observed in red blood cells (RBCs) count, mean corpuscular volume (MCV), haemoglobin (Hb) concentration, mean corpuscular Hb (MCH), red cell distribution width (RDW), hematocrit (HCT) mean corpuscular Hb concentration (MCHC) and mean PLT volume (MPV). Radiation treatment increased thiobarbituric acid-reactive substances (TBARS) levels, catalase and superoxide dismutase (SOD) activities, and caused hepatic and renal damage. Oral administration of F. carica before irradiation significantly increased WBC, PLT, lymphocytes and neutrophils counts, along with a decrease in TBARS levels, and catalase and SOD activities in serum, liver, and kidney. These results suggested that consumption of F. carica a natural product with antioxidant capacity and capability to quench singlet oxygen could help mitigate cellular damage caused by whole-body irradiation-induced free radicals.

Keywords

Ionizing radiation Ficus carica Hematology Oxidative stress Experimental animals 

Notes

Acknowledgments

Authors extend their appreciation to College of Science, King Saud University; for providing all facilities to complete this work.

Funding information

This research project was supported by a grant from the “Research Center of the Female Scientific and Medical Colleges”, Deanship of Scientific Research, King Saud University.

Compliance with ethical standards

Conflict of interest

The authors have indicated that they have no conflict of interest regarding the content of this article.

Ethical consideration

All procedures contributing to this work comply with the ethical standards of the relevant national guides on the care and use of laboratory animals and have been approved and authorized by the Institutional Animal Care and Use Committee (IACUC) at King Saud University, Riyadh, Saudi Arabia.

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Copyright information

© Institute of Zoology, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Zoology Department, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Zoology and Entemology Department, Faculty of ScienceHelwan UniversityHelwanEgypt
  3. 3.Pharmacology and Toxicology Department, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Pharmacology and Toxicology Department, College of PharmacyAin Shams UniversityCairoEgypt
  5. 5.Biochemistry Department, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  6. 6.Genetic Engineering Division, Molecular Biology DepartmentNational Research CentreCairoEgypt
  7. 7.Zoology Department, Faculty of ScienceCairo UniversityCairoEgypt

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