Evaluation of DNA damage in haemolymph of freshwater mussels Unio pictorum from Lake Skadar
- 13 Downloads
This study was carried out on mussels Unio pictorum as test organism, in order to identify the possible pollution in Moraca River and Lake Skadar. Lake Skadar, located in a karst area in the outer part of the south-eastern Dinaric Alps, is the largest of the Balkan lakes and has a surface area which fluctuates seasonally from approximately 370 to 600 km2. The mussels U. pictorum were exposed in cages by 30 individuals on several locations for the period of three weeks. Following the three-week exposure, haemolymph samples were taken from mussels and tested using the Comet assay and micronucleus test. DNA damage on living organisms, made by pressure of pollutans and other stresors were evaluated. In brief, the results of investigation show that DNA damage is certainly present in caged U. pictorum mussels, and that it is caused by pollution in the Moraca River, which affects ecological and toxicological status of Lake Skadar.
KeywordsUnio pictorum Lake Skadar Comet assay Micronucleus test
We acknowledge the support by Ministry of Science of Montenegro and Croatian Ministry of Science and Technology for the research bilateral project.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Beeton AM, Karaman G (1981) The biota and limnology of Lake Skadar. Titograd GRO Prosveta, BeogradGoogle Scholar
- Bolognesi C, Cirillo S (2014) Gentotoxicity biomarkers in aquatic bioindicators. Curr Zool 60:273–284. https://doi.org/10.1093/czoolo/60.2.273
- Kolarević S, Kolarevic-Kostic J, Slobodnik J, Liška I, Gačić Z, Paunović M, Vukčević-Knežević J, Gačić-Vuković B (2015) Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: the joint Danube survey 3. Sci Total Environ 540:377–385. https://doi.org/10.1016/j.scitotenv.2015.06.061 CrossRefPubMedGoogle Scholar
- Kosmehl T, Krebs F, Manz W, Erdinger L, Braunbeck T, Hollert H (2004) Comparative genotoxicity testing of Rhine River sediment extracts using the Comet assay with permanent fish cell lines (RTG-2 and RTL-W1) and the Ames test. JSS 4(2):84–94. https://doi.org/10.1065/JSS2004.03.095
- Kostanjsek R, Lapanje A, Drobne D, Perović S, Perović A, Zidar P, Strus J, Hollert H, Karaman G (2005) Bacterial community structure analyses to assess pollution of water and sediments in the Lake Shkodra/Skadar, Balkan Peninsula. Environ Sci Pollut Res 12(6): 361–368. https://doi.org/10.1065/espr2005.07.271
- Rastall AC, Neziri A, Vukovic Z, Jung C, Mijovic S, Hollert H, Nikcevic S, Erdinger L (2004) The identification of readily bioavailable pollutions in Lake Shkodra/Skadar using Semipermeable membrane devices (SPMDs), bioassays and chemical analysis. Environ Sci Pollut Res 11(4):240–253. https://doi.org/10.1065/espr2004.03.188
- Skadarsko jezero (Site 3YU003) In: A directory of wetlands of International importance 2002. The Ramsar Convention Bureau. Gland, SwitzerlandGoogle Scholar
- Štambuk A, Pavlica M, Vignjević G, Bolarić B, Klobučar G (2009) Assessment og genotoxicity in polluted freshwaters using caged painters mussel, Unio pictorum. Ecotoxicology 18:430–439. https://doi.org/10.1007/s10646-009-0297-2
- Stesevic D, Feiler U, Sundic D, Mijovic S, Erdinger L, Seiler TB, Heininger P, Hollert H (2007) Application of a new sediment contact test with Myriophyllum aquaticum and of the aquatic Lemna test to assess the sediment quality of Lake Skadar. J Soils Sediments 7(5):342–349. https://doi.org/10.1065/jss2007.08.249 CrossRefGoogle Scholar