Comparative overview of red kidney bean (Phaseolus valgaris) rhizospheric bacterial diversity in perspective of altitudinal variations

Suyal, Deep Chandra; Kumar, Saurabh; Joshi, Divya; Yadav, Amit; Shouche, Yogesh; Goel, Reeta

doi:10.2478/s11756-019-00292-1

Original Article
Published: 19 June 2019

Comparative overview of red kidney bean (Phaseolus valgaris) rhizospheric bacterial diversity in perspective of altitudinal variations

Deep Chandra Suyal¹,
Saurabh Kumar¹,
Divya Joshi¹,
Amit Yadav²,
Yogesh Shouche² &
Reeta Goel¹

Biologia volume 74, pages 1405–1413 (2019)Cite this article

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Abstract

Red kidney bean (RKB) is an important cash crop of the Western Indian Himlayan (WIH) regions. Despite of having a long cultivation history, very little has known about its rhizospheric microbial diversity, which is becoming important for employing sustainable agricultural plans. In this perspective, the bacterial diversity and community structure of Himalayan RKB rhizosphere were studied and compared across the altitudinal zones of WIH. Metagenomic analysis revealed that Proteobacteria (31%) was the major phylum with the dominance of the genera Sphingomonas in the Garhwal while Pseudomonas in the Kumaun regions of Western Indian Himalaya (WIH), followed by Bacteroidetes (20%), Fermicutes (9%), Acidobacteria (7%), Actinobacteria (6%), Chloroflexi (5%), Gemmatimonadetes (3%) and Planctomyces (2%). Furthermore, the Garhwal soil was found to harbor more bacterial diversity than the Kumaun with unique genera Conexibacter, Ornithobacterium, Lacibacter, Salinibacter, Fervidicola, Haliangium, Pirellula, Caloramator, Desulfitobacterium, Edaphobacter, Holophaga and Massilia. The principal component analysis revealed that the bacterial diversity was associated with the content of “total solids” as well as “volatile acids” present in the respective soils. Additionally, large number of unclassified clone sequences suggesting the need of culture dependent studies to trap “yet not cultivated” bacterial species. Conclusively, the present study provides an overview of RKB associated bacterial diversity which must be explored for sustainable hill agricultural plans.

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Acknowledgements

The author (DCS) acknowledges the Science and Engineering Research Board (SERB) young scientist scheme, Grant No. YSS/2015/001214 during the course of this study. Also Senior Research Fellowship (CSIR Award No: 09/171(0126)/2015-EMR-I) to SK is acknowledged. Dr. Prabha Pant, Assistant professor, Department of Humanities & Social Sciences, GBPUAT Pantnagar is duly acknowledged for manuscript editing and improvement.

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Authors and Affiliations

Department of Microbiology, College of Basic Sciences and Humanities, G.B.P.U.A&T, Pantnagar, Uttarakhand, 263145, India
Deep Chandra Suyal, Saurabh Kumar, Divya Joshi & Reeta Goel
National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, Maharashtra, 411021, India
Amit Yadav & Yogesh Shouche

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Deep Chandra Suyal
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Saurabh Kumar
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Divya Joshi
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Amit Yadav
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Yogesh Shouche
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Reeta Goel
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Correspondence to Reeta Goel.

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Suyal, D.C., Kumar, S., Joshi, D. et al. Comparative overview of red kidney bean (Phaseolus valgaris) rhizospheric bacterial diversity in perspective of altitudinal variations. Biologia 74, 1405–1413 (2019). https://doi.org/10.2478/s11756-019-00292-1

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Received: 17 December 2018
Accepted: 04 June 2019
Published: 19 June 2019
Issue Date: October 2019
DOI: https://doi.org/10.2478/s11756-019-00292-1

Keywords

Red kidney bean
Himalayan agro-ecosystems
16S rDNA
Bacterial diversity
Rhizosphere