pp 1–14 | Cite as

The anti-invasive activity of Robinia pseudoacacia L. and Amorpha fruticosa L. on breast cancer MDA-MB-231 cell line

  • Danijela M. CvetkovićEmail author
  • Jovana V. Jovankić
  • Milena G. Milutinović
  • Danijela D. Nikodijević
  • Filip J. Grbović
  • Andrija R. Ćirić
  • Marina D. Topuzović
  • Snežana D. Marković
Original Article


The paper investigates anticancer effects of methanol extracts of invasive plant species Robinia pseudoacacia L. (RpE) and Amorpha fruticosa L. (AfE) on breast cancer MDA-MB-231 and healthy MRC-5 cells. The anticancer activity was evaluated through examination of cytotoxic effects, anti-invasive potential and impact on redox status in comparative analysis using their chemical composition. According to the IC50 values, the investigated plants had no significant cytotoxic effects either on healthy cell line MRC-5 or on MDA-MB-231 cancer cells, but they showed great anti-invasive potential by suppressing all investigated parameters of tumor invasion and metastases (Matrix Metalloproteinases (MMP), protein concentration and MMP-9, C-X-C Motif Chemokine Ligand 12 (CXCL-12), Vascular Endothelial Growth Factor (VEGF-A) and Hypoxia-Inducible Factor (HIF-1α) gene expression) in MDA-MB-231 cells. Based on their remarkable anti-invasive potential, RpE and AfE are suitable for use as potential supplements in anticancer therapy or as nutritional food supplements.


Amorpha fruticosa L. Anti-invasive potential Anticancer activity Robinia pseudoacaciaBreast cancer 



Robinia pseudoacacia L. extract


Amorpha fruticosa L. extract


Plasminogen activator


nitric oxide synthase


Vascular Endothelial Growth Factor


Hypoxia-Inducible Factor


C-X-C Motif Chemokine Ligand 12


Matrix metalloproteinases-9


Reactive oxygen species


Reactive nitrogen species


Human epidermal growth factor receptor-2



This work was supported by the Ministry of Science and Technological Development of the Republic of Serbia (project III41010 and III41007). We are grateful to Milica Pešić on donating MRC-5 cell line.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Danijela M. Cvetković
    • 1
    Email author
  • Jovana V. Jovankić
    • 1
  • Milena G. Milutinović
    • 1
  • Danijela D. Nikodijević
    • 1
  • Filip J. Grbović
    • 1
  • Andrija R. Ćirić
    • 2
  • Marina D. Topuzović
    • 1
  • Snežana D. Marković
    • 1
  1. 1.Faculty of Science, Department for Biology and EcologyUniversity of KragujevacKragujevacSerbia
  2. 2.Faculty of Science, Department for ChemistryUniversity of KragujevacKragujevacSerbia

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