Skip to main content
Log in

Green synthesis of silver nanoparticles using Hyptis suaveolens (L.) Poit leaf extracts, their characterization and cytotoxicity evaluation against PC-3 and MDA-MB 231 cells

  • Original Article
  • Published:
Biologia Aims and scope Submit manuscript

A Correction to this article was published on 24 May 2019

This article has been updated


The green synthesis of nanoparticles using plant extracts is an efficient eco-friendly approach. The present study is aimed at investigating the green synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Hyptis suaveolens and evaluation of their cytotoxicity against cancer cells. The formation of AgNPs upon incubation of AgNO3 with leaf extract was identified by color change (yellow to brown), further confirmed by characteristic UV-visible absorbance peak at 436 nm. The Extended dispersive X-ray spectroscopy in turn confirmed elemental silver content in green synthesized AgNPs (G.S.AgNPs). The scanning and transmission electron microscopy revealed randomly dispersed spherical shaped particles in a size range of 20–80 nm. The X-ray diffraction spectroscopy has shown distinct Bragg diffraction peaks corresponding to the planes of face centered cubic lattice, indicating crystalline nature of G.S.AgNPs. The Dynamic light scattering spectroscopy has shown average size distribution of NPs ranging from 80 to 207 nm, certainly higher as it measures the size of entire complexion of organic capping layer with the nanoparticle. The Zeta potential of G.S.AgNPs in water was found to be −13.7 mV, reflecting their higher stability. Further, the Fourier transform infrared spectroscopy revealed the leaf extract’s phenolic compounds and certain proteins responsible for the biological reduction of Ag+ and stabilization of AgNPs. The MTT assay has shown prominent cytotoxic activity of G.S.AgNPs against PC-3 and MDA-MB 231 cells with IC50 values of 63.16 and 52.49 μg/ml respectively. Overall, the study clearly indicates efficient synthesis of AgNPs, which can have a scope for the application in cancer therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Change history

  • 24 May 2019

    The article was published with an erroneous rendering of Fig. 1b.


Download references


The author great fully acknowledge the financial assistance from University Grants Commission, New Delhi, India under the scheme of UGC-National Fellowship for Students of Other Backward Classes (OBC).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Satyanarayana Botcha.

Ethics declarations

Conflict of interest

All the authors declare no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Botcha, S., Prattipati, S.D. Green synthesis of silver nanoparticles using Hyptis suaveolens (L.) Poit leaf extracts, their characterization and cytotoxicity evaluation against PC-3 and MDA-MB 231 cells. Biologia 74, 783–793 (2019).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: