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Morphological and molecular characterization of two populations of Aphelenchus avenae (Nematoda: Aphelenchidae) from Iran and their phylogenetic relationships

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Abstract

Soil samples were collected from the rhizosphere of faba bean (Vicia faba L.) in Khuzestan province, south-western Iran. Many of the localities were positive for the presence of Aphelenchus avenae. The morphological and morphometric characters of the two Iranian populations are discussed and illustrated based on morphological and morphometrics data. Additionally, sequences of the D2-D3 expansion segments of 28S rRNA gene for two populations were used for molecular phylogenetic analysis. One population was without male and formed a separate branch in the phylogenetic tree. Results indicate high genetic variation among A. avenae populations from different geographic regions.

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Acknowledgments

The author gratefully thanks Dr. Irma Tandingan De Ley (Department of Nematology, University of California, Riverside, USA) for providing some useful articles and the Shahid Chamran University of Ahvaz for financial support.

Funding

This study was funded by Shahid Chamran University of Ahvaz (grant number 1276).

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box 61357-83151, Ahvaz, Iran

    Sedighe Azimi

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  1. Sedighe Azimi
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Correspondence to Sedighe Azimi.

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Azimi, S. Morphological and molecular characterization of two populations of Aphelenchus avenae (Nematoda: Aphelenchidae) from Iran and their phylogenetic relationships. Biologia 73, 683–691 (2018). https://doi.org/10.2478/s11756-018-0079-5

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