A novel alphabaculovirus isolated from the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae): characterization and pathogenicity
The cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a polyphagous pest that exist all over the world. It is also very destructive in Turkey on various agricultural products. In this study, we have detected an alphabaculovirus from Helicoverpa armigera larvae collected from cotton field. The virus isolate was named as Helicoverpa armigera nucleopolyhedrovirus (HearNPV-O1) based on morphological and molecular properties. This is the first record of baculovirus from H. armigera in Eurasia region. Scanning electron microscopy examinations of polyhedral inclusion bodies (PIBs) showed their irregular morphology with average diameter of 0.85 to 1.25 μm. Transmission electron microscopy studies revealed that the nucleocapsids were multiple enveloped and bacilliform shaped. The size of nucleocapsid was found as 279 nm with a width of 56 nm. Digestion of viral genome by PstI generated 8 fragments with total size of 130.9 kbp. According to the phylogenetic analysis of the partial polyhedrin (polh), late expression factor 8 (lef8) and late expression factor 9 (lef9) genes, HearNPV-O1 isolate is very close to H. armigera MNPV Indian isolate-443. Five different concentrations of HearNPV-O1 between 103 and 107 were used in dose-response test on neonate, 3rd and 5th instars larvae of H. armigera. The highest dose (107) showed 92%, 88% and 57% mortality, respectively within 14 days. LC50 values of HearNPV-O1 isolate were calculated as 6 × 104, 7 × 104 and 8 × 106 PIBs/mL−1 against neonate, 3rd and 5th instars larval stages, respectively. These results demonstrate that HearNPV-O1 isolate may be a potential biocontrol agent to be utilized against H. armigera.
KeywordsAlphabaculovirus Helicoverpa armigera Microbial control Insect viruses NPV
We would like to thank to the Department of Metallurgical and Materials Engineering in Karadeniz Technical University for assistance with scanning electron microcopy studies.
This study was financially supported by Karadeniz Technical University (Project No: BAP06 FDK2016–5468).
Compliance with ethical standards
Conflict of interest
No potential conflict of interest was reported by the authors.
- Adams JR, McClintock JT (1991) Nuclear polyhedrosis viruses of insects. In: Adams JR, Bonami JR (eds) Atlas of invertebrate viruses. CRC Press, Boca Raton, pp 87–204Google Scholar
- Al-ssum RM, Marie MA (2009) In vitro response of host cell to Chlamydia pneumonia infection, an ultra structure investigation. Iran J Microbiol 1:31–36Google Scholar
- Bayramoglu Z, Gencer D, Muratoglu H, Efe D, Nalcacioglu R, Demirbag Z, Demir I (2017) Characterization of a nucleopolyhedrovirus variant of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae) in Turkey. Int J Pest Manag. https://doi.org/10.1080/09670874.2017.1344789
- Beas-Catena A, Sánchez-Mirón A, García-Camacho F, Contreras-Gómez A, Molina-Grima E (2014) Baculovirus bio-pesticides: An overview. J Anim Plant Sci 24:362–373Google Scholar
- Cunningham JT (1998) North America. In: Hunter-Fujita FR, Entwistle PF, Evans HF, Crook NE (eds) Insect viruses and Pest management. John Wiley & Sons, New York, pp 313–331Google Scholar
- Demir I, Demirbag Z (2006) A productive replication of Hyphantria cunea nucleopolyhedrovirus in Lymantria dispar cell line. J Microbiol Biotechnol 16:1485–1490Google Scholar
- Demir I, Nalcacioglu R, Mohammed Gholizad L, Demirbag Z (2014) A highly effective nucleopolyhedrovirus against Malacosoma spp. (Lepidoptera: Lasiocampidae) from Turkey: isolation, characterization, phylogeny, and virulence. Turk J Agric For 38:462–470. https://doi.org/10.3906/tar-1307-32 CrossRefGoogle Scholar
- Figueiredo E, Muñoz D, Escribano A, Mexia A, Vlak JM, Caballero P (1999) Biochemical identification and comparative insecticidal activity of nucleopolyhedrovirus pathogenic for Heliothis armigera (Lep., Noctuidae) larvae. J Appl Entomol 123:165–169. https://doi.org/10.1046/j.1439-0418.1999.00336.x CrossRefGoogle Scholar
- Gencer D, Bayramoglu Z, Nalcacıoglu R, Kleespies RG, Demirbag Z, Demir I (2018) Characterization of three alphabaculovirus isolates from the gypsy moth, Lymantria dispar dispar (Lepidoptera: Erebidae), in Turkey. Biocontrol Sci Tech. https://doi.org/10.1080/09583157.2017.1420752
- Grzywacz D, Rabindra RJ, Brown M, Jones KAJ, Parnell M (2004) Helicoverpa armigera NPV production manual. Natural Resources Institute, Chatham, p 107Google Scholar
- Hemati SA, Naseri B, Razmjou J (2013) Reproductive performance and growth indices of cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) on various host plants. J Crop Prot 2:193–208Google Scholar
- Herniou EA (2003) Use of comparative genomics and Phylogenetics to study the evolution of the Baculoviridae. PhD Thesis, Department of Biological Sciences, Imperial College, London, 84Google Scholar
- Joussen N, Agnolet S, Lorenz S, Schöne SE, Ellinger R, Schneider B, Heckel DG (2012) Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. Proc Natl Acad Sci 109:15206–15211. https://doi.org/10.1073/pnas.1202047109 CrossRefPubMedPubMedCentralGoogle Scholar
- Kaur B, Gupta VK, Jindal V (2014) Molecular phylogenetic analysis of Indian strain of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Indian J Biotechnol 13:186–194Google Scholar
- Miller LK (1996) Insect viruses. In: Fields BN, Knipe DM, Howley PM (eds) Fundamental Virology. Lippincott-Raven Publishers, Philadelphia, pp 401–424Google Scholar
- Mironidis GK, Kapantaidaki D, Bentila M, Morou E, Savopoulou-Soultani M, Vontas J (2013) Resurgence of the cotton bollworm Helicoverpa armigera in northern Greece associated with insecticide resistance. Insect Science 20:505–512. https://doi.org/10.1111/j.1744-7917.2012.01528.x CrossRefPubMedGoogle Scholar
- Moore SD, Pittaway T, Bouwer G, Fourie JG (2004) Evaluation of Helicoverpa armigera nucleo polyhedron virus (HearNPV) for control of Helicoverpa armigera (Lepidoptera: Noctuidae) on citrus in South Africa. Biocontrol Sci Tech 14:239–250. https://doi.org/10.1080/09583150310001655666 CrossRefGoogle Scholar
- Nagarkatti S, Prakash A (1974) Rearing of Heliothis armigera (Hubner) on an artificial diet. Techonol Bull 17:169–173Google Scholar
- Ogembo JG, Caoili BL, Shikata M, Chaeychomsri S, Kobayashi M, Ikeda M (2009) Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp NPVs. Virus Genes 9:261–272. https://doi.org/10.1007/s11262-009-0389-3 CrossRefGoogle Scholar
- Raghavendra AT, Jalali SK, Ojha R, Shivalingaswamy TM, Bhatnagar R (2017) Whole genome sequence and comparative genomic sequence analysis of Helicoverpa armigera nucleopolyhedrovirus (HearNPV-L1) isolated from India. Virus Disease 28:61–68. https://doi.org/10.1007/s13337-016-0352-6 CrossRefPubMedPubMedCentralGoogle Scholar
- Reddy T, Sannaveerappanava V (2011) Bio-efficiency of commercial neem products and HaNPV against bollworms of cotton. International journal of farm. Sciences 1:105–110Google Scholar
- Rowley DL, Popham HJ, Harrison RL (2011) Genetic variation and virulence of nucleopolyhedroviruses isolated worldwide from the heliothine pests Helicoverpa armigera, Helicoverpa zea, and Heliothis virescens. J Invertebr Pathol 107:112–126. https://doi.org/10.1016/j.jip.2011.03.007 CrossRefPubMedGoogle Scholar
- Szewczyk B, de Souza ML, de Castro MEB, Moscardi ML, Moscardi F (2011) Baculovirus biopesticides. In: Stoytcheva M (ed) Pesticides-formulations, effects, fate. InTech Publishing, Rijeka, Crotia, pp 25–36Google Scholar