Skip to main content
Springer Nature Link
Log in

Mitochondrial (COI) and nuclear (EF-1α) DNA variability of Rhopalosiphum padi and Rhopalosiphum nymphaeae (Hemiptera: Aphididae) in Lithuania

  • Section Zoology
  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Partial sequences of mitochondrial COI and nuclear EF-1α genes have been analysed across the Lithuanian samples of Rhopalosiphum padi and R. nymphaeae (Hemiptera: Aphididae). In addition, samples from the adjacent countries of the Eastern Baltic region of Europe (Latvia, Estonia and Poland) and available sequences from GenBank and Bold Systems were used. The range of intraspecific pairwise R. padi COI sample (n = 44) divergences (K2P model) was 0.00–2.49% (average 0.61%). Out of 10 haplotypes, one was predominant (n = 25), with Worldwide distribution. The range of intraspecific pairwise R. nymphaeae COI sample (n = 20) divergences (K2P model) was 0.00–1.31% (average 0.22%). Out of 6 haplotypes, one was predominant (n = 13), sampled in Lithuania, Ukraine and Turkey (Asian part). The range of intraspecific pairwise sample divergences (K2P model) of EF-1α fragment was 0.00–0.40% (average 0.10%) for R. padi (n = 20) and 0.00–0.60% (average 0.13%) for R. nymphaeae (n = 17). Two COI haplotypes (including the Worldwide distributed one) and three EF-1α haplotypes (including the most common one) were detected in Lithuanian populations of R. padi. The present study suggests that R. padi populations from the Eastern Baltic region are predominantly holocyclic.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Blackman R.L. & Eastop V.F. 1984. Aphids on the World’s Crops. John Wiley & Sons, Ltd., Chichester, 466 pp. ISBN: 9780471904267

    Google Scholar 

  • Blackman R.L. & Eastop V.F. 2000. Aphids on the World’s Crops: An Identification and Information Guide. 2nd Ed. John Wiley & Sons, Ltd., Chichester, 466 pp. ISBN-10: 0471851914, ISBN-13: 978-0471851912

    Google Scholar 

  • Blackman R.L. & Eastop V.F. 2006. Aphids on the World’s Herbaceous Plants and Shrubs. John Wiley & Sons, Ltd., Chichester, 1439 pp. ISBN-13: 978-0471489733, ISBN-10: 0471489735

    Google Scholar 

  • Bulman S.R., Stufkens M.A.W., Eastop V.F. & Teulon D.A.J. 2005. Rhopalosiphum aphids in New Zealand. II. DNA sequences reveal two incompletely described species. N. Z. J. Zool. 32(1): 37–45. DOI: 10.1080/03014223.2005.9518396

    Article  Google Scholar 

  • Carter M.J., Simon J.-C. & Nespolo R.F. 2012. The effects of reproductive specialization on energy costs and fitness genetic variances in cyclical and obligate parthenogenetic aphids. Ecol. Evol. 2(7): 1414–1425. DOI: 10.1002/ece3.247

    Article  PubMed Central  PubMed  Google Scholar 

  • Clement M., Posada D. & Crandall K.A. 2000. TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9(10): 1657–1659. DOI: 10.1046/j.1365-294x.2000.01020.x

    Article  CAS  PubMed  Google Scholar 

  • Delmotte F., Sabater-Muñoz B., Prunier-Leterme N., Latorre A., Sunnucks P., Rispe C. & Simon J.C. 2003. Phylogenetic evidence for hybrid origins of asexual lineages in an aphid species. Evolution 57(6): 1291–1303. DOI: http://dx.doi.org/10.1554/02-557

    Article  CAS  PubMed  Google Scholar 

  • Dixon A.F.G. 1971. The life-cycle and host preferences of the bird cherry-oat aphid, Rhopalosiphum padi L., and their bearing on the theories of host alternation in aphids. Ann. Appl. Biol. 68(2): 135–147. DOI: 10.1111/j.1744-7348.1971.tb06450.x

    Article  CAS  PubMed  Google Scholar 

  • Favret C. 2014. Aphid Species File. Aphid.speciesfile.org:Taxon Name:26101. http://aphid.speciesfile.org/HomePage/Aphid/HomePage.aspx (accessed 28.03.2014)

    Google Scholar 

  • Folmer O., Black M., Hoeh W., Lutz R. & Vrijehoek R. 1994. DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 3(5): 294–299. PMID: 7881515

    CAS  PubMed  Google Scholar 

  • Foottit R.G., Maw H.E.L., von Dohlen C.D. & Herbert P.D.N. 2008. Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes. Mol. Ecol. Resour. 8(6): 1189–1201. DOI: 10.1111/j.1755-0998.2008.02297.x

    Article  CAS  PubMed  Google Scholar 

  • Gilabert A., Simon J.C., Mieuzet L., Halkett F., Stoeckel S., Plantegenest M. & Dedryver C.A. 2009. Climate and agricultural context shape reproductive mode variation in an aphid crop pest. Mol. Ecol. 18(14): 3050–3061. DOI: 10.1111/j.1365-294X.2009.04250.x

    Article  CAS  PubMed  Google Scholar 

  • Halkett F., Plantegenest M., Bonhomme J. & Simon J.C. 2008. Gene flow between sexual and facultatively asexual lineages of an aphid species and the maintenance of reproductive mode variation. Mol. Ecol. 17(12): 2998–3007. DOI: 10.1111/j.1365-294X.2008.03798.x

    Article  CAS  PubMed  Google Scholar 

  • Halkett F., Plantegenest M., Prunier-Leterme N., Mieuzet L., Delmotte F. & Simon J.C. 2005. Admixed sexual and facultatively asexual aphid lineages at mating sites. Mol. Ecol. 14(1): 325–336. DOI: 10.1111/j.1365-294X.2004.02358.x

    Article  CAS  PubMed  Google Scholar 

  • Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

    CAS  Google Scholar 

  • Hance T., Nibelle D., Lebrun P., Van Impe G. & Van Hove C. 1994. Selection of Azolla forms resistant to the water lily aphid, Rhopalosiphum nymphaeae. Life history of Rhopalosiphum nymphaeae. Entomol. Exp. Appl. 70(1): 11–17. DOI: 10.1111/j.1570-7458.1994.tb01754.x

    Article  Google Scholar 

  • Heie O.E. 1986. The Aphidoidea (Hemiptera) of Fennoscandia and Denmark III, Aphididae: Pterocommatinae and Aphidinae (Aphidini). Fauna Entomol. Scand. 17: 314 pp. ISBN: 9789004080881

  • Holman J. 2009. Host Plant Catalog of Aphids — Palaearctic Region. Springer, Berlin, 1216 pp. ISBN: 978-1-4020-8285-6. DOI: 10.1007/978-1-4020-8286-3

    Book  Google Scholar 

  • Hullé M., Maurice D., Rispe C., & Simon J.C. 1999. Clonal variability in sequences of morph production during the transition from parthenogenetic to sexual reproduction in the aphid Rhopalosiphum padi (Sternorrhyncha: Aphididae). Eur. J. Entomol. 96(2): 125–134.

    Google Scholar 

  • Kim H. & Lee S. 2008. A molecular phylogeny of the tribe Aphidini (Insecta: Hemiptera: Aphididae) based on the mitochondrial tRNA/COII, 12S/16S and the nuclear EF1alpha genes. Syst. Entomol. 33(4): 711–721 DOI: 10.1111/j.1365-3113.2008.00440.x

    Article  Google Scholar 

  • Kim H., Lee S. & Jang Y. 2011 Macroevolutionary patterns in the Aphidini aphids (Hemiptera: Aphididae): diversification, host association, and biogeographic origins. PLoS ONE 6(9): E24749. DOI: 10.1371/journal.pone.0024749

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kudirkaitė-Akulienė V. & Rakauskas R. 2009. Contribution to the knowledge of aphid (Hemiptera: Aphididae) fauna of Šakiai district, Lithuania. Acta Zool. Lituan. 19(3): 235–243. DOI: 10.2478/v10043-009-0020-9

    Article  Google Scholar 

  • Leather S.R. & Dixon A.F.G. 1981. Growth, survival and reproduction of the bird-cherry aphid, Rhopalosiphum padi, on its primary host. Ann. Appl. Biol. 99(2): 115–118. DOI: 10.1111/j.1744-7348.1981.tb05136.x

    Article  Google Scholar 

  • Leather S.R., Walters K.F.A. & Dixon A.F.G. 1989. Factors determining the pest status of the bird cherry-oat aphid, Rhopalosiphum padi (L.) (Hemiptera: Aphididae) in Europe: a study and review. Bull. Entomol. Res. 79(3): 345–360. DOI: http://dx.doi.org/10.1017/S0007485300018344

    Article  Google Scholar 

  • Lee W., Kim H., Lim J., Choi H.R., Kim Y., Kim Y.S., Ji J.Y., Foottit R.G. & Lee S. 2011. Barcoding aphids (Hemiptera: Aphididae) of the Korean Peninsula: updating the global data set. Mol. Ecol. Resour. 11(1): 32–37. DOI: 10.1111/j.1755-0998.2010.02877.x.

    Article  PubMed  Google Scholar 

  • Nespolo R.F., Halkett F., Figueroa C.C., Plantegenest M. & Simon J.C. 2009. Evolution of trade-offs between sexual and asexual phases and the role of reproductive plasticity in the genetic architecture of aphid life histories. Evolution 63(9): 2402–2412. DOI: 10.1111/j.1558-5646.2009.00706.x

    Article  PubMed  Google Scholar 

  • Nieto Nafria J.M., Andreev A.V., Binazzi A., Mier Durante M.P., Perez Hidalgo N. J., Rakauskas R. & Stekolshchikov A.V. 2004. Superfamily Aphidoidea. In: Fauna Europaea Service. http://www.faunaeur.org / http://www.faunaeur.org/fullresults.php?id=12358

    Google Scholar 

  • Ollivier M., Gabaldón T., Poulain J., Gavory F., Leterme N., Gauthier J.-P., Legeai F., Tagu D., Simon J.C. & Rispe C. 2012. Comparison of gene repertoires and patterns of evolutionary rates in eight aphid species that differ by reproductive mode. Genome Biol. Evol. 4(2): 155–167. DOI: 10.1093/gbe/evr140

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Oraze M.J. & Grigarick A.A. 1992. Biological control of ducksalad (Heteranthera limosa) by the water lily aphid (Rhopalosiphum nymphaeae) in rice (Oryza sativa). Weed Science 40(2): 333–336.

    Google Scholar 

  • Pike K.S. & Schaffner R.L. 1985. Development of autumn populations of cereal aphids, Rhopalosiphum padi (L.) and Schizaphis graminum (Rondani) (Homoptera: Aphididae) and their effects on winter wheat in Washington State. J. Econom. Entomol. 78(3): 676–680.

    Google Scholar 

  • Plumb R.T. 1983. Barley yellow dwarf virus — a global problem, pp. 185–194. In: Plumb R.T. & Thresh J.M. (eds), Plant Virus Epidemiology — The Spread and Control of Insect-borne Viruses, Blackwell Scientific Publications, Oxford, United Kingdom, 350 pp. ISBN-10: 0632010282, ISBN-13: 978-0632010288

    Google Scholar 

  • Posada D. 2008. jModelTest: Phylogenetic Model Averaging. Mol. Biol. Evol. 25(7): 1253–1256. DOI: 10.1093/molbev/msn083

    Article  CAS  PubMed  Google Scholar 

  • Rakauskas R. & Cichocka E. 2005. Aphids inhabiting Prunus in the Eastern Baltic region: present state of knowledge and prospective research, pp. 141–152. In: Wilkaniec B. et al. (eds), Monograph Aphids and other Hemipterous Insects [Mszyce i inne pluskwiaki], 11, Polish Aphidological Group, Agricultural University of Poznan, Poland, 211 pp. ISBN: 83-89887-47-9

    Google Scholar 

  • Rakauskas R. & Trukšinaitė J. 2011. Preliminary list of aphid (Hemiptera: Aphididae, Adelgidae) species of Trakai district, Lithuania. Acta Zool. Lituan. 21(1): 52–62. DOI: 10.2478/v10043-011-0009-z

    Article  Google Scholar 

  • Rakauskas R., Rupais A. & Juronis V. 1992. Lietuvos amar (Homoptera, Aphidoidea) sąrašas. [The check-list of Lithuanian Aphidodea], pp. 83–100. In: Jonaitis V. (ed.), Naujos ir retos Lietuvos vabzdžių rūšys. 1992 metų pranešimai ir aprašymai [New and rare for Lithuanian SSR insect species. Records and descriptions of 1992], Leidiniai Vol. 12, Vilnius: Lithuanian Entomologists Society, Institute of Ecology.

    Google Scholar 

  • Remaudière G. & Remaudière M. 1997. Catalogue des Aphididae du Monde. Techniques et Pratiques. INRA, Paris, 473 pp. ISBN: 2738007147, 9782738007148

    Google Scholar 

  • Ronquist F. & Huelsenbeck J.P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19(12): 1572–1574. DOI: 10.1093/bioinformatics/btg180

    Article  CAS  PubMed  Google Scholar 

  • Ronquist F., Huelsenbeck J.P. & van der Mark P. 2005. MrBayes 3.1 Manual, 69 pp.

    Google Scholar 

  • Ruszkowska M. 2002. Przekształcenia cyklicznej partenogenezy mszycy Rhopalosiphum padi (L.) (Homoptera: Aphidoidea) — znaczenie zjawiska w adaptacji środowiskowej [Alternation in parthenogenesis of the aphid Rhopalosiphum padi (L.) (Homoptera: Aphidoidea) — creative adaptation of resource]. Rozprawy Naukowe Instytutu Ochrony Roślin (Poznań) 8: 1–63. ISBN: 8391620433, 9788391620434

    Google Scholar 

  • Simon J.C., Le Gallic J.F., Bonhomme J. & Dedryver C.A. 1994. Breeding system complex in the cereal aphid Rhopalosiphum padi (Homoptera: Aphididae) and its influences on population biology and dynamics. Bulletin OILB/SROP 17(4): 11–15.

    Google Scholar 

  • Smatas R. 2006. The occurrence and control of aphids and thrips in winter triticale. Agronomy Res. 4(Spec iss.): 379–383.

    Google Scholar 

  • Smatas R., Tamosiunas K., Semaskiene R., Dabkevicius Z. & Lazauskas S. 2008. Trends in aphid occurrence in spring barely in 1976–2007. J. Plant Prot. Res. 48(3): 275–282. DOI: 10.2478/v10045-008-0036-3

    Article  Google Scholar 

  • Stroyan H.L.G. 1984. Aphids — Pterocommatinae and Aphidinae (Aphidini). Handbook for the Identification of British Insects 2(6), 232 pp. ISBN-10: 0901546623, ISBN-13: 978-0901546623

    Google Scholar 

  • Surkus J. 1997. Insecticides against pests on spring barley. Zemdirbyste — Agriculture 60: 142–152. [in Lithuanian]

    Google Scholar 

  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., & Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28(10): 2731–2739. DOI: 10.1093/molbev/msr121

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tatchell G.M., Plumb R.T. & Carter N. 1988. Migration of alate morphs of the bird cherry aphid (Rhopalosiphum padi) and implications for the epidemiology of barley yellow dwarf virus. Ann. Appl. Biol. 112(1): 1–11. DOI: 10.1111/j.1744-7348.1988.tb02035.x

    Article  Google Scholar 

  • Turčinavičienė J., Pedersen B.V. & Rakauskas R. 2006. Phylogenetic relationships in the “grossulariae” species group of the genus Aphis (Hemiptera: Sternorrhyncha: Aphididae): Molecular evidence. Eur. J. Entomol. 103(3): 597–604 DOI: 10.14411/eje.2006.080

    Article  Google Scholar 

  • Valenzuela I., Eastop V. F., Ridland P.M. & Weeks A.R. 2009. Molecular and morphometric data indicate a new species of the aphid genus Rhopalosiphum (Hemiptera: Aphididae). Ann. Entomol. Soc. Amer. 102(6): 914–924. DOI: http://dx.doi.org/10.1603/008.102.0602

    Article  CAS  Google Scholar 

  • Valenzuela I., Ridland P.M., Weeks A.R. & Hoffmann A.A. 2010. Patterns of genetic variation and host adaptation in an invasive population of Rhopalosiphum padi (Hemiptera: Aphididae). Ann. Entomol. Soc. Amer. 103(6): 886–897. DOI: http://dx.doi.org/10.1603/AN09113

    Article  Google Scholar 

  • van Emden H.F. & Harrington R. 2007. Aphids as Crop Pests. CABI, Wallingford, Oxford, UK, 717 pp. ISBN: 1845932021, 9781845932022

    Book  Google Scholar 

  • von Dohlen C.D. & Teulon D.A.J. 2003. Phylogeny and historical biogeography of New Zealand indigenous Aphidini aphids (Hemiptera, Aphididae): A hypothesis. Ann. Entomol. Soc. Amer. 96(2): 107–116. DOI: http://dx.doi.org/10.1603/0013-8746(2003)096[0107:PAHBON]2.0.CO;2

    Article  Google Scholar 

  • Wickerman G.P. & Wratten S.D. 1979. The biology and pest status of cereal aphids (Hemiptera: Aphididae) in Europe: a review. Bull. Entomol. Res. 69(1): 1–32. DOI: http://dx.doi.org/10.1017/S0007485300017855

    Article  Google Scholar 

  • Wiktelius S. & Chiverton P.A. 1985. Ovariole number and fecundity for the two emigrating generations of the bird cherry-oat aphid (Rhopalosiphum padi) in Sweden. Ecol. Entomol. 10(3): 349–355. DOI: 10.1111/j.1365-2311.1985.tb00731.x

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, Faculty of Natural Sciences, Vilnius University, Čiurlionio str 21/27, LT 03101, Vilnius, Lithuania

    Rimantas Rakauskas, Jekaterina Havelka & Rasa Bernotienė

Authors
  1. Rimantas Rakauskas
    View author publications

    Search author on:PubMed Google Scholar

  2. Jekaterina Havelka
    View author publications

    Search author on:PubMed Google Scholar

  3. Rasa Bernotienė
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Rimantas Rakauskas.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rakauskas, R., Havelka, J. & Bernotienė, R. Mitochondrial (COI) and nuclear (EF-1α) DNA variability of Rhopalosiphum padi and Rhopalosiphum nymphaeae (Hemiptera: Aphididae) in Lithuania. Biologia 69, 1730–1741 (2014). https://doi.org/10.2478/s11756-014-0475-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11756-014-0475-4

Key words