Skip to main content
SpringerLink
Log in

Cytology of the genus Artemisia (Anthemidae, Asteraceae) in the Western Himalayas

  • Section Botany
  • Published:
Biologia Aims and scope Submit manuscript

Abstract

The present study revealed the varied frequency of natural chromosomal abnormalities in 13 populations pertaining to 9 species of the genus Artemisia L. from different localities of Himachal Pradesh (Western Himalaya). Intraspecific chromosome variability has been reported for the first time on worldwide basis in Artemisia vestita (2n = 2x = 36) and from India in A. macrocephala (2n = 2x = 18) and A. scoparia (2n = 2x = 36). Besides, B-chromosomes have been reported here for the first time in A. nilagirica and A. roxburghiana. Most of the populations show anomalous meiotic behaviour resulting in cytomixis, chromosomal stickiness, unoriented bivalents, formation of laggards and bridges which leads to abnormal microsporogenesis, and production of heterogeneous-sized fertile pollen grains along with reduced pollen fertility.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ashraf M., Hayat H.Q., Shazia Jabeen S., Shaheen N., Khan M.A. & Yasmin G. 2010. Artemisia L. species recognized by the local community of northern areas of Pakistan as folk therapeutic plants. J. Med. Plants Res. 4: 112–119.

    Google Scholar 

  • Badr A., El-Shazly H.H., Helail N.S. & Ghanim W.E. 2012. Genetic diversity of Artemisia populations in central and north Saudi Arabia based on morphological variation and RAPD polymorphism. Plant Syst. Evol. DOI:10.1007/s00606-012-0597-5.

    Google Scholar 

  • Bakshi S.K. 1982. Presence of B Chromosomes in Artemisia vulgaris. Nucleus (Calcutta) 25: 116–118.

    Google Scholar 

  • Bakshi S.K. & Kichloo S. 1985. B-chromosomes in Artemisia maritima L. Chrom. Ing. Ser. 38: 23–35.

    Google Scholar 

  • Bala S. & Gupta R.C. 2011. In Marhold, K. (ed.). IAPT/IOPB chromosome data 12. Taxon 60: 1784–1786.

    Google Scholar 

  • Bala S., Kaushal B. & Gupta R.C. 2012. Effect of B-chromosomes on meiotic behaviour and genetic recombination in Artemisia nilagirica (C.B. Clarke) Pamp. Chromosome Bot. 7: 9–12.

    Article  Google Scholar 

  • Baptista-Giacomelli F.R., Pagliarini M.S. & Almeida J.L. 2000. Meiotic behavior in several Brazilian oat cultivars (Avena sativa L.). Cytologia 65: 371–378.

    Article  Google Scholar 

  • Baqur S.R. & Akhtar S. 1968. Cytological studies of the genus Sesbania from W. Pakistan. Cytologia 33: 427–438

    Article  Google Scholar 

  • Bellucci M., Roscini C. & Mariani A. 2003. Cytomixis in pollen mother cells of Medicago sativa L. J. Hered. 94: 512–516

    Article  PubMed  CAS  Google Scholar 

  • Bhat B.K., Bakshi S.K. & Kaul M.K. 1974. In IOPB chromosome number reports XLVI. Taxon 23: 801–812.

    Google Scholar 

  • Brickell C. (ed.) 2003. RHS A-Z Encyclopedia of Garden Plants. Third edition. Dorling Kindersley, London, ISBN 0-7513-3738-2.

    Google Scholar 

  • Cardini F. & Haung W. 1998. Moxibustion for correction of breech presentation: a randomized controlled trial. JAMA 280: 1580–84.

    Article  PubMed  CAS  Google Scholar 

  • Dar G.H., Bhagat R.C. & Khan M.A. 2002. Biodiversity of the Kashmir Himalaya. Anmol Publications, New Delhi, 418 pp.

    Google Scholar 

  • Efferth T., Dunstan H., Sauerbrey A., Miyachi H. & Chitambar C.R. 2001. The anti-malarial artesunate is also active against cancer. Int. J. Oncol. 18: 767–73.

    PubMed  CAS  Google Scholar 

  • Fadaei F., Sheidai M. & Asadi M. 2010. Cytological study of the genus Arenaria L. (Caryophyllaceae). Caryologia 63: 149–156

    Article  Google Scholar 

  • Falistocco E., Tosti N. & Falcinelli M. 1995. Cytomixis in pollen mother cells of diploid Dactylis one of the origins of 2n gametes. J. Hered. 86: 448–453.

    Google Scholar 

  • Garcia S., Garnatje T., Dariimaa Sh., Tsooj Sh. & Vallès J. 2006. New or rarely reported chromosome numbers in taxa of subtribe Artemisiinae (Anthemideae, Asteraceae) from Mongolia. Bot. J. Linn. Soc. 150: 203–210.

    Article  Google Scholar 

  • Ghaffari S.M. 2006. Occurrence of diploid and polyploid microspores in Sorghum bicolor (Poaceae) is the result of cytomixis. Afr. J. Biotechnol. 5: 1450–1453.

    Google Scholar 

  • Gupta R.C., Kataria V. & Mehra A. 2012. Cytomorphological studies in some gamopetalous species of Western Himalaya: an attempt to add new or varied cytotypes. Chromosome Bot. 7: 59–65.

    Article  Google Scholar 

  • Gupta R.C., Himshikha, Rana P.K., Kumar P. & Singhal V.K. 2010. First report of structural heterozygosity in Artemisia parviflora (Asteraceae) from Parvati Valley in Kullu District (Himachal Pradesh, India). Bot Serb. 34: 63–66.

    Google Scholar 

  • Gupta R.C., Mehra A. & Singh V. 2013. Cyto-morphology of Some Gamopetalous species from Jammu Area (Jammu & Kashmir), India. J. Jpn. Bot. 88: 108–114.

    Google Scholar 

  • Haider S.Z., Andola H.C. & Mohan M. 2012. Constituents of Artemisia gmelinii Weber ex Stechm. from Uttarakhand Himalaya: A Source of Artemisia Ketone. Indian J. Pharm. Sci. 74: 265–267.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Hajra P.K., Rao R.R., Singh D.K. & Uniyal B.P. 1995. Flora of India, vol. 12 & 13. Botanical Survey of India, Calcutta.

    Google Scholar 

  • Iqbal Z., Lateef M., Ashraf M. & Jabbar A. 2004. Anthelmintic activity of Artemisia brevifolia in sheep. J. Ethnopharmacol. 93: 265–268.

    Article  PubMed  Google Scholar 

  • Jeelani S.M., Rani S., Kumar S., Kumari S. & Gupta R.C. 2011. Meiotic studies in some members of Caryophyllaceae Juss. from the Western Himalayas. Acta Biol. Craco.-Ser. Bot. 53: 86–95.

    Google Scholar 

  • Kaul M.K. & Bakshi S.K. 1984. Studies on the genus Artemisia L. in North-West Himalaya with particular reference to Kashmir. Folia Geobot. Phytotax. 19: 299–316.

    Google Scholar 

  • Khoshoo T.N. & Sobti S.N. 1958. Cytology of Indian species of Artemisia. Nature 181: 853–854.

    Article  Google Scholar 

  • Ko Y.D., Kim J.H., Adesogan A.T., Ha H.M. & Kim S.C. 2006. The effect of replacing rice straw with dry wormwood (Artemisia sp.) on intake, digestibility, nitrogen balance and ruminal fermentation characteristics in sheep. Animal Feed Sci. Technol. 125: 99–110.

    Article  CAS  Google Scholar 

  • Koul M.L.H. 1964a. Chromosome numbers in some medicinal composites. Proc. Indian Acad. Sci., Sec. B 59: 72–77.

    Google Scholar 

  • Koul M.L.H. 1964b. Cytogenetics of polyploids I. Cytology of A. vulgaris L. Cytologia 29: 407–414.

    Article  Google Scholar 

  • Kumar P. & Singhal V.K. 2011. Chromosome number, male meiosis and pollen fertility in selected angiosperms of the cold deserts of Lahaul-Spiti and adjoining areas (Himachal Pradesh, India). Plant Syst. Evol. 297: 271–297.

    Article  Google Scholar 

  • Kumar S., Jeelani S.M., Rani S., Gupta R.C. & Kumari S. 2013. Cytology of five species of subfamily Papaveroideae from the Western Himalayas. Protoplasma 250: 307–316.

    Article  PubMed  Google Scholar 

  • Lai H. & Singh N.P. 2006. Oral artemisinin prevents and delays the development of 7,12-dimethylbenz (a) anthracene (DMBA)-induced breast cancer in the rat. Cancer Lett. 231: 43–48.

    Article  PubMed  CAS  Google Scholar 

  • Marks G.E. 1954. An aceto-carmine glycerol jelly for use in pollen-fertility counts. Stain Technol. 29(5): 277.

    PubMed  CAS  Google Scholar 

  • Martín J., Torrell M., Korobkov A.A. & Vallès J. 2003. Palynological features as a systematic marker in Artemisia L. and related genera (Asteraceae, Anthemideae) II: Implications for subtribe Artemisiinae delimitation. Plant Biology 5: 85–93.

    Article  Google Scholar 

  • Mathew A. & Mathew P.M. 1988. Cytological studies on the south Indian Compositae. Glimpses Pl. Res. 8: 1–177.

    Google Scholar 

  • Mehra P.N. & Remanandan P. 1969. In IOPB chromosome number reports XXII. Taxon 18: 433–442.

    Google Scholar 

  • Mehra P.N. & Remanandan R. 1974. Cytological investigations on Indian Compositae II. Astereae, Heliantheae, Helenieae and Anthemideae. Caryologia 27: 255–284.

    Article  Google Scholar 

  • Moore R.J. 1973, 1974, 1977. Index to plant chromosome numbers. 1967–1971, 1972. 1973–74. Regnum Veg 90, 91, 96.

    Google Scholar 

  • Nirmala A. & Rao P.N. 1996. Genetics of chromosome numerical mosaicism in higher plants. Nucleus 39: 151–175.

    Google Scholar 

  • Nirmala A. & Rao P.N. 1984. Karyotype studies in Asteraceae. Cell Chromosome Res. 7: 26–28.

    Google Scholar 

  • Pellicer J., Garcia S., Garnatje T., Dariimma S., Korobkov A.A. & Vallès J. 2007. Chromosome numbers in some Artemisia (Asteracceae, Antehmideae) species and genome size variation in its subgenus Dracunculus: Karyological, systematics and phylogenetic implications. Chromosome Bot. 2: 45–54.

    Article  Google Scholar 

  • Peng C.I. & Hsu C.C. 1978. Chromosome number in Taiwan Compositae. Bot. Bull. Acad. Sin 19: 53–66.

    Google Scholar 

  • Probatova N.S. 2000. Chromosome numbers in some plant species from the Razdolnaya (Suifun) river basin (Primorsky Territory). Bot. Žhurn. (Moscow & Leningrad) 85(12): 102–107.

    Google Scholar 

  • Proksch P. 1992. Artemisia, pp. 357–377. In: Hansel R.. Keller K., Rimpler H., Schneider G. & Hrsg (eds), Hagers Handbuch der Pharmazeutischen Praxis. Springer-Verlag, Berlin.

    Google Scholar 

  • Thomson J.D. 1995. Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytol. 129: 1–22.

    Article  Google Scholar 

  • Sheidai M. & Fadaei F. 2005. Cytogenetic studies in some Bromus L. species sec. Genea J Genet. 84: 189–194.

    Article  Google Scholar 

  • Sheidai M., Koobaz P. & Zehzad B. 2003. Meiotic studies of some Avena L. species and populations. Iran J Sci. 14: 121–131.

    Google Scholar 

  • Sheidai M., Nikoo M. & Gholipour A. 2008. Cytogenetic variability and new chromosome number reports in Silene L. species (Sect. Lasiostemones, Caryophyllaceae). Acta Biol Szeged. 52: 313–319.

    Google Scholar 

  • Stewart R.R. 1972. An annotated catalogue of the vascular plants of West Pakistan and Kashmir. In: Nasir E. & Ali S.I. (eds), Fakhri Press, Karachi, 1028 pp.

  • Vallès J., Torrell M., Garnatje T., Garcia-Jacas N., Vilatersana R. & Susanna A. 2003. Genus Artemisa and its allies, phylogeny of the subtribe Artemisiinae (Asteraceae, Anthemidae) based on nucleotide sequences of nuclear ribosomal DNA internal transcribed spacers (ITS), Plant Biol. 5: 274–284.

    Article  Google Scholar 

  • Vallès J. & Garnatje T. 2005. Artemisia and its allies: genome organization and evolution and their biosystematic, taxonomic and phylogenetic implication in the Artimisiinae and related subtribes (Asteraceae, Anthemideae), pp. 255–285. In: Sharma A.K. & Sharma A. (eds), Plant genome: biodiversity and evolution. Vol. 1B: Phenarogams (Higher Groups). M/S Science publishers, Enfield.

    Google Scholar 

  • Villeux R. 1985. Diploid and polyploid gametes in Crop Plants: Mechanisms of formation and utilization in plant breeding. In: Janick J. (ed.), Plant Breed Rev 3, AVI Publishing Co. Wesport, Connecticut 442 pp.

    Google Scholar 

  • Wang W.M. 2004. On the origin and development of Artemisia (Asteraceae) in the geological past. J. Linn. Soc. Bot. 145: 331–336.

    Article  Google Scholar 

  • Xiong X., Ling Y.R. & Jiang L. 1995. Studies on the chromosome numbers and karyotypes of six species of Artemisia (Compositae). J. Trop. Subtrop. Bot. 3: 23–29.

    Google Scholar 

  • Zheng H., Wu Y, Ding J., Binion D., Fu W. & Reardon R. 2006. Artemisia vulgaris: Mugwort, pp. 21–27. In: Ford-Werntz D., Strazanac J. & Baudoin A. (eds), Invasive plants of Asian origin established in the United States and their natural enemies, vol.1. Forest Health Technology Enterprise Team, Morgantown, WV, USA.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, Punjabi Univerity, Patiala, India

    Raghbir Chand Gupta, Henna Goyal & Vijay Singh

Authors
  1. Raghbir Chand Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Henna Goyal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vijay Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vijay Singh.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gupta, R.C., Goyal, H. & Singh, V. Cytology of the genus Artemisia (Anthemidae, Asteraceae) in the Western Himalayas. Biologia 69, 1134–1141 (2014). https://doi.org/10.2478/s11756-014-0413-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11756-014-0413-5

Key words

Search

Navigation