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Biologia

, Volume 69, Issue 6, pp 713–721 | Cite as

Recent insight in α-glucan metabolism in probiotic bacteria

  • Marie S. Møller
  • Yong Jun Goh
  • Alexander H. Viborg
  • Joakim M. Andersen
  • Todd R. Klaenhammer
  • Birte Svensson
  • Maher Abou Hachem
Review Section Cellular and Molecular Biology
  • 198 Downloads

Abstract

α-Glucans from bacterial exo-polysaccharides or diet, e.g., resistant starch, legumes and honey are abundant in the human gut and fermentation of resistant fractions of these α-glucans by probiotic lactobacilli and bifidobacteria impacts human health positively. The ability to degrade polymeric α-glucans is confined to few strains encoding extracellular amylolytic activities of glycoside hydrolase (GH) family 13. Debranching pullulanases of the subfamily GH13_14 are the most common extracellular GH13 enzymes in lactobacilli, whereas corresponding enzymes are mainly α-amylases and amylopullulanases in bifidobacteria. Extracellular GH13 enzymes from both genera are frequently modular and possess starch binding domains, which are important for efficient catalysis and possibly to mediate attachment of cells to starch granules. α-1,6-Linked glucans, e.g., isomalto-oligosaccharides are potential prebiotics. The enzymes targeting these glucans are the most abundant intracellular GHs in bifidobacteria and lactobacilli. A phosphoenolpyruvate-dependent phosphotransferase system and a GH4 phospho-α-glucosidase are likely involved in metabolism of isomaltose and isomaltulose in probiotic lactobacilli based on transcriptional analysis. This specificity within GH4 is unique for lactobacilli, whereas canonical GH13 31 α-1,6-glucosidases active on longer α-1,6-gluco-oligosaccharides are ubiquitous in bifidobacteria and lactobacilli. Malto-oligosaccharide utilization operons encode more complex, diverse, and less biochemically understood activities in bifidobacteria compared to lactobacilli, where important members have been recently described at the molecular level. This review presents some aspects of α-glucan metabolism in probiotic bacteria and highlights vague issues that merit experimental effort, especially oligosaccharide uptake and the functionally unassigned enzymes, featuring in this important facet of glycan turnover by members of the gut microbiota.

Key words

prebiotic oligosaccharide uptake ATP-binding cassette transport system phosphotransferase system transcriptional analysis isomalto-oligosaccharide malto-oligosaccharide 

Abbreviations

ABC

ATP-binding cassette

CAZy

Carbohydrate-Active enZymes database

CBM

carbohydrate binding module

GH

glycoside hydrolase

GI

gastrointestinal tract

GT

glycosyl transferase family

IMO

isomaltooligosaccharide

PTS

phosphotransferase transport system

RS

resistant starch

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2014

Authors and Affiliations

  • Marie S. Møller
    • 1
  • Yong Jun Goh
    • 2
  • Alexander H. Viborg
    • 1
  • Joakim M. Andersen
    • 1
  • Todd R. Klaenhammer
    • 2
  • Birte Svensson
    • 1
  • Maher Abou Hachem
    • 1
  1. 1.Enzyme and Protein Chemistry, Department of Systems BiologyTechnical University of DenmarkKgs. LyngbyDenmark
  2. 2.Department of Food, Bioprocessing and Nutrition SciencesNorth Carolina State UniversityRaleighUSA

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