Advertisement

Biologia

, Volume 68, Issue 6, pp 1022–1027 | Cite as

Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp.

  • Leya Thomas
  • Raveendran Sindhu
  • Ashok Pandey
Section Cellular and Molecular Biology
  • 160 Downloads

Abstract

Xylanases constitute an important industrial enzyme, which hydrolyzes the polysaccharide xylan. In this work, a novel Streptomyces strain producing cellulase-free xylanase was isolated from the soil samples collected from the mangrove forest of Kadalundi, Kerala, India. The strain produced unique enzyme, which exhibited optimal activity at pH 9.0 and tolerance up to pH 12.0. Media engineering was carried out to improve the enzyme production, which showed best enzyme production at 30°C, medium pH 9.0 and incubation time of 48 h. Enzyme was highly thermo-tolerant up to 70°C and alkaline tolerant. Partial gene amplification as well as partial purification of enzyme was carried out to characterize the enzyme. The unique features of the enzyme make it an ideal candidate for industrial application for paper and pulp industry.

Key words

xylanase Streptomyces sp. alkaline tolerance thermo-tolerance paper and pulp industry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdelwahed N.A.M., El-Naggar N.E. & Saber W.I.A. 2011. Factors and correlations controlling cellulase-free xylanase production by Streptomyces halstedii NRRL B-1238 in submerged culture. Aust. J. Basic Appl. Sci. 5: 45–53.Google Scholar
  2. Altschul S.F., Gish W., Miller W., Myers E.W. & Lipman D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.PubMedGoogle Scholar
  3. Bailey M.J., Beily P.& Poutanen K. 1992. Interlaboratory testing and methods for assay of xylanase activity. J. Biotechnol. 23: 257–270.CrossRefGoogle Scholar
  4. Beg Q.K., Bharat B., Mukesh K. & Hoondal G.S. 2000. Enhanced production of a thermostable xylanase from Streptomyces sp. QG-11-3 and its application in biobleaching of eucalyptus kraft pulp. Enzyme Microb. Technol. 27: 459–466.PubMedCrossRefGoogle Scholar
  5. Beg Q.K., Kapoor M., Mahajan L. & Hoondal G.S. 2001. Microbial xylanases and their industrial applications: a review. Appl. Microbiol. Biotechnol. 56: 326–338.PubMedCrossRefGoogle Scholar
  6. Benson D.A., Cavanaugh M., Clark K., Karsch-Mizrachi I., Lipman D.J., Ostell J. & Sayers E.W. 2013. GenBank. Nucleic Acids Res. 41(Database issue): D36–D42.PubMedCrossRefGoogle Scholar
  7. Collins T., Gerday C. & Feller G. 2005. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol. Rev. 29: 3–23.PubMedCrossRefGoogle Scholar
  8. Corpet F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16: 10881–10890.PubMedCrossRefGoogle Scholar
  9. Dhanasekaran D., Sivamani P., Arunagrinathan N., Paneerselvum A. & Thajuddin N. 2005. Screening and identification of antibiotic producing strains of marine Streptomyces. J. Microbial World 7: 62–66.Google Scholar
  10. Dhiman S.S., Sharma J. & Battan B. 2008. Industrial applications and future prospects of microbial xylanases: a review. BioResources 3: 1377–1402.Google Scholar
  11. Ghose T.K. 1987. Measurement of cellulase activities. Pure Appl. Chem. 59: 257–268.CrossRefGoogle Scholar
  12. Gupta B.N., Mishra S. & Basak U.C. 2007. Occurrence of Streptomyces aurantiacus in mangroves of Bhitarkanika. Malaysian J. Microbiol. 3: 7–14.Google Scholar
  13. Johnson L.I. & Curl E.A. 1972. Methods for Research on Ecologyof Soil Born Pathogens. Burgess Pub. Co., Minneapolis, 247 pp.Google Scholar
  14. Kumar A., Gupta P., Shrivastava B., Khosa Y.P. & Kuhad R.C. 2012. Xylanase production from an alkalophilic actinomycete isolate Streptomyces sp. RCK 2010, its characterization and application in saccharification of second generation biomass. J. Mol. Catal. B Enzymatic 74: 170–177.CrossRefGoogle Scholar
  15. Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.PubMedCrossRefGoogle Scholar
  16. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., Lopez R., Thompson J.D., Gibson T.J. & Higgins D.G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948.PubMedCrossRefGoogle Scholar
  17. Lescic I., Zehl M., Muller R., Vukelic B., Abramic M., Pigac J., Allmaier G. & Kojicprodic B. 2004. Structural characterization of extracellular lipase from Streptomyces rimosus: assignment of disulphide bridge pattern by mass spectrometry. Biol. Chem. 385: 1147–1156.PubMedCrossRefGoogle Scholar
  18. Maheswari M.U. & Chandra T.S. 2000. Production and potential applications of a xylanase from a new strain of Streptomyces cuspidosporus. World J. Microbiol. Biotechnol. 16: 257–263.CrossRefGoogle Scholar
  19. Miller G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428.CrossRefGoogle Scholar
  20. Ninawe S., Lal R. & Kuhad R.C. 2006. Isolation of three xylanase producing strains of actinomycetes and their identification using molecular methods. Curr. Microbiol. 53: 178–182.PubMedCrossRefGoogle Scholar
  21. Peixoto-Nogueira S., Michelin M., Betini J.H.A., Jorge J.A., Terenzi H.F. & Polizeli M.L.T.M. 2009. Production of xylanase by Aspergilli using alternative carbon sources: application of the crude extract on cellulose pulp biobleaching. J. Ind. Microbiol. Biotechnol. 36: 149–155.CrossRefGoogle Scholar
  22. Ratanakhanokchai K., Kyu K.L. & Tanticharoen M. 1999. Purification and properties of a xylan-binding endoxylanase from alkalophilic Bacillus sp. strain K-1. Appl. Environ. Microbiol. 65: 694–697.PubMedGoogle Scholar
  23. Saratale G.D., Sartale R.G. & Koh S.E. 2012. Production and characterization of multiple cellulolytic enzymes by isolated Streptomyces sp. MDS. Biomass Bioenergy 47: 302–315.CrossRefGoogle Scholar
  24. Sharma P. & Bajaj B.K. 2006. Production and partial characterization of alkali tolerant xylanases from an alkalophilic Streptomyces sp. CD3. J. Sci. Ind. Res. 64: 688–697.Google Scholar
  25. Singh R., Kapoor V. & Kumar V. 2012. Utilization of agroindustrial waste for the simultaneous production of amylase and xylanases by thermophilic actinomycetes. Braz. J. Microbiol. 43: 1545–1552.PubMedCrossRefGoogle Scholar
  26. Thomas L. Arumugam M. & Pandey A. 2013. Production, purification, characterization and over-expression of xylanases from actinomycetes. Ind. J. Expt. Biol.Google Scholar
  27. Uyar F. & Baysal Z. 2004. Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation. Process Biochem. 39: 1893–1898.CrossRefGoogle Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2013

Authors and Affiliations

  1. 1.Biotechnology DivisionNational Institute for Interdisciplinary Science and Technology, CSIRTrivandrumIndia

Personalised recommendations